Other Stains

Question 1

mixture of picric acid and acid fuchsin for demonstration of connective tissues.

Answer 1

Van Gieson’s Stain

Question 2

used to stain collagen, smooth muscle, or
mitochondria

Answer 2

Masson Stain

Question 3

Masson Stain is used as the nuclear and cytoplasmic stain in

Answer 3

Mallory’s trichrome method

Question 4

In Van Gieson’s picro-fuchsin, acid fuchsin imparts _____ color to collagen fibers

Answer 4

red

Question 5

1% Acid fuchsin 13 ml Saturated aqueous picric acid 87 ml

Answer 5

Picro-fuchsin solution

Question 6

a basic acridine fluorochrome which permits
discrimination between dead and living cells

Answer 6

acridine orange

Question 7

acridine orange imparts _____ fluorescence in DNA

Answer 7

green

Question 8

acridine orange imparts _____ fluorescence in RNA

Answer 8

red

Question 9

demonstrate deposits of calcium salts and possible sites of phosphatase activities

Answer 9

Acridine Red 3B

Question 10

a complex, water-soluble phthalocyanin dye, similar to chlorophyll

Answer 10

Alcian Blue

Question 11

Alcian blue is stains

Answer 11

acid mucopolysaccharides
connective tissue
epithelial mucin

Question 12

Alcian blue is often combine wtih

Answer 12

PAS

Question 13

Alcian blue + PAS stains acidic mucins

Answer 13

blue

Question 14

Alcian blue + PAS stains neutral mucins

Answer 14

red

Question 15

forms an orange-red lake with calcium at a pH of 4.2

Answer 15

Alizarin Red S

Question 16

Alizarin Red S works best with small amounts of calcium, such as in

Answer 16

Michaelis-Gutman bodies

Question 17

also used on the Dupont ACA analyzer to measure serum calcium photometrically

Answer 17

Alizarin method

Question 18

cytoplasmic stain used for counterstaining of
epithelial sections

Answer 18

Aniline blue

Question 19

Nuclei are deep red; cytoplasm is a pale red.

Answer 19

Azocarmine

Question 20

used for staining hemoglobin

Answer 20

benzidine

Question 21

used as a contrast stain for Gram’s technique, in acid fast and Papanicolau method, and for staining diphtheria organisms

Answer 21

Bismarck Brown

Question 22

chromatin stain for fresh materials in smear preparations

Answer 22

Carmine

Question 23

The most important component of carmine is _________

Answer 23

carminic acid

Question 24

A _____ is often prepared in the form of carmine aluminum calcium lake (“carmine alum lake”)

Answer 24

dry powder

Question 25

a mordanted dye acting as a basic dye and staining acidic substances

Answer 25

Carmalum (Mayer’s Solution)