Other Stains Flashcards
mixture of picric acid and acid fuchsin for demonstration of connective tissues.
Van Gieson’s Stain
used to stain collagen, smooth muscle, or
mitochondria
Masson Stain
Masson Stain is used as the nuclear and cytoplasmic stain in
Mallory’s trichrome method
In Van Gieson’s picro-fuchsin, acid fuchsin imparts _____ color to collagen fibers
red
1% Acid fuchsin 13 ml Saturated aqueous picric acid 87 ml
Picro-fuchsin solution
a basic acridine fluorochrome which permits
discrimination between dead and living cells
acridine orange
acridine orange imparts _____ fluorescence in DNA
green
acridine orange imparts _____ fluorescence in RNA
red
demonstrate deposits of calcium salts and possible sites of phosphatase activities
Acridine Red 3B
a complex, water-soluble phthalocyanin dye, similar to chlorophyll
Alcian Blue
Alcian blue is stains
acid mucopolysaccharides
connective tissue
epithelial mucin
Alcian blue is often combine wtih
PAS
Alcian blue + PAS stains acidic mucins
blue
Alcian blue + PAS stains neutral mucins
red
forms an orange-red lake with calcium at a pH of 4.2
Alizarin Red S
Alizarin Red S works best with small amounts of calcium, such as in
Michaelis-Gutman bodies
also used on the Dupont ACA analyzer to measure serum calcium photometrically
Alizarin method
cytoplasmic stain used for counterstaining of
epithelial sections
Aniline blue
Nuclei are deep red; cytoplasm is a pale red.
Azocarmine
used for staining hemoglobin
benzidine
used as a contrast stain for Gram’s technique, in acid fast and Papanicolau method, and for staining diphtheria organisms
Bismarck Brown
chromatin stain for fresh materials in smear preparations
Carmine
The most important component of carmine is _________
carminic acid
A _____ is often prepared in the form of carmine aluminum calcium lake (“carmine alum lake”)
dry powder
a mordanted dye acting as a basic dye and staining acidic substances
Carmalum (Mayer’s Solution)
