Methylene Blue

Question 1

common basic nuclear stain

Answer 1

methylene blue

Question 2

methylene blue usually contains some

Answer 2

azure
methyl violet

Question 3

can be substituted for

Answer 3

Janus Green B stain
Carmine Stain

Question 4

involves the oxidation of methylene blue, resulting in loss of methyl groups and leaving lower homologues of the dye (azures) and deaminized oxidation products (thiazoles)

Answer 4

polychroming

Question 5

resulting mixture of methylene blue, azures and thiazoles

Answer 5

polychrome methylene blue

Question 6

methylene blue stains nuclei

Answer 6

blue

Question 7

methylene blue stains cartilage matrix, mucin, mast cell granules and connective tissues

Answer 7

reddish-violet color

Question 8

valuable stain for plasma cells & may also be employed in cytological examinations of fresh sputum for malignant cells, as a bacterial stain for evaluation and differentiation of bacterial organisms, for diagnosis of diphtheria, and for
vital staining of the nervous tissue

Answer 8

polychrome methylene blue:

Question 9

originally known as Eosin- Methylene Blue (EMB) method

Answer 9

Mallory’s Phloxine Methylene Blue Stain

Question 10

metachromatic dye formed whenever methylene blue is heated in fixed alkali or alkali carbonate

Answer 10

methylene violet

Question 11

a lipophilic stain; it will accumulate in
lipid globules inside cells, staining them red.

Answer 11

Nile Red

Question 12

a dye that is more soluble in fat than in water
or alcohols -> stain for neutral lipids

Answer 12

Oil Red O

Question 13

an excellent stain for elastic fibers, and is especially recommended in dermatological studies

Answer 13

Orcein

Question 14

a selective stain for unsaturated lipids and for
lipoproteins such as myelin

Answer 14

Osmium Tetroxide

Question 15

Osmium tetroxide stains

Answer 15

black

Question 16

an all-around useful stain for many things

Answer 16

Periodic Acid Schiff

Question 17

The aldehydes are then detected by the Schiff
reagent, which stains them

Answer 17

reddish purple

Question 18

a common negative stain for viruses, nerves,
polysaccharides, and other biological tissue
materials

Answer 18

phosphotungstic acid

Question 19

phosphotungstic acid is an ideal stain for the demonstration of striated muscle fibers and mitochondria, which stain

Answer 19

blue

Question 20

employed as a contrast stain to acid fuchsin, for the demonstration of connective tissue (Van Gieson’s stain)

Answer 20

picric acid

Question 21

picric acid is a cytoplasmic stain in contrast to

Answer 21

basic dyes

Question 22

picric acid is a as a counterstain to

Answer 22

crystal violet

Question 23

T/F. picric acid is a tissue fixative, and as a decalcifying agent

Answer 23

true

Question 24

an insoluble colored salt of ferric ferrocyanide (an iron cyanide compound) normally utilized for the manufacture of paints

Answer 24

prussian blue

Question 25

Prussian blue is used for microanatomical color contrast of specimens and for demonstration of the _____ and _____ by injection (intravital staining).

Answer 25

blood lymph vessels

Question 26

used with osmic acid to fix and stain blood and glandular tissue

Answer 26

Rhodamine B

Question 27

a nuclear stain produces red nuclei, and is used primarily as a counterstain

Answer 27

Safranin

Question 28

Safranin gives a _____ color to collagen

Answer 28

yellow

Question 29

used in 10% aqueous solution to prepare various dilutions

Answer 29

Silver Nitrate

Question 30

Silver nitrate is used in identifying

Answer 30

spirochetes reticulum other fiber stains

Question 31

a nuclear stain for fixed tissues

Answer 31

toluidine blue

Question 32

toluidine blue is used as a substitute for _____ in fresh frozen tissue sections

Answer 32

thionine

Question 33

toluidine blue is recommended for staining of

Answer 33

Nissl granules chromophilic bodies

Question 34

Van Gieson binds to collagen in the extracellular matrix, staining it

Answer 34

pink

Question 35

Van Gieson is often combined with a stain for elastic fibers (elastic van Gieson) which stain _____

Answer 35

black

Question 36

used for demonstration of neuroglia in frozen sections

Answer 36

victoria blue

Question 37

a silver reduction method that demonstrates phosphates and carbonates, but these are usually present along with calcium

Answer 37

Von Kossa stain

Question 38

causes blood cells to exhibit four major staining properties that allow the cell types to be distinguished

Answer 38

wright stain

Question 39

affinity for methylene blue color product:

Answer 39

basophilia very dark bluish purple

Question 40

affinity for the oxidation products of methylene blue called azures, which are reddish purple

Answer 40

azurophilia

Question 41

affinity for eosin color product:

Answer 41

acidophilia red to red-orange

Question 42

affinity for a complex of dyes in the mixture, which are reddish brown to pale lilac

Answer 42

neutrophilia