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MTAP 2 - HISTOPATH - FINALS EXAM - ADDTL READINGS > Methylene Blue > Flashcards

Methylene Blue Flashcards

1
Q

common basic nuclear stain

A

methylene blue

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2
Q

methylene blue usually contains some

A

azure
methyl violet

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3
Q

can be substituted for

A

Janus Green B stain
Carmine Stain

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4
Q

involves the oxidation of methylene blue, resulting in loss of methyl groups and leaving lower homologues of the dye (azures) and deaminized oxidation products (thiazoles)

A

polychroming

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5
Q

resulting mixture of methylene blue, azures and thiazoles

A

polychrome methylene blue

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6
Q

methylene blue stains nuclei

A

blue

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7
Q

methylene blue stains cartilage matrix, mucin, mast cell granules and connective tissues

A

reddish-violet color

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8
Q

valuable stain for plasma cells & may also be employed in cytological examinations of fresh sputum for malignant cells, as a bacterial stain for evaluation and differentiation of bacterial organisms, for diagnosis of diphtheria, and for
vital staining of the nervous tissue

A

polychrome methylene blue:

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9
Q

originally known as Eosin- Methylene Blue (EMB) method

A

Mallory’s Phloxine Methylene Blue Stain

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10
Q

metachromatic dye formed whenever methylene blue is heated in fixed alkali or alkali carbonate

A

methylene violet

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11
Q

a lipophilic stain; it will accumulate in
lipid globules inside cells, staining them red.

A

Nile Red

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12
Q

a dye that is more soluble in fat than in water
or alcohols -> stain for neutral lipids

A

Oil Red O

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13
Q

an excellent stain for elastic fibers, and is especially recommended in dermatological studies

A

Orcein

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14
Q

a selective stain for unsaturated lipids and for
lipoproteins such as myelin

A

Osmium Tetroxide

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15
Q

Osmium tetroxide stains

A

black

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16
Q

an all-around useful stain for many things

A

Periodic Acid Schiff

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17
Q

The aldehydes are then detected by the Schiff
reagent, which stains them

A

reddish purple

18
Q

a common negative stain for viruses, nerves,
polysaccharides, and other biological tissue
materials

A

phosphotungstic acid

19
Q

phosphotungstic acid is an ideal stain for the demonstration of striated muscle fibers and mitochondria, which stain

A

blue

20
Q

employed as a contrast stain to acid fuchsin, for the demonstration of connective tissue (Van Gieson’s stain)

A

picric acid

21
Q

picric acid is a cytoplasmic stain in contrast to

A

basic dyes

22
Q

picric acid is a as a counterstain to

A

crystal violet

23
Q

T/F. picric acid is a tissue fixative, and as a decalcifying agent

A

true

24
Q

an insoluble colored salt of ferric ferrocyanide (an iron cyanide compound) normally utilized for the manufacture of paints

A

prussian blue

25
Q

Prussian blue is used for microanatomical color contrast of specimens and for demonstration of the _____ and _____ by injection (intravital staining).

A

blood
lymph vessels

26
Q

used with osmic acid to fix and stain blood and
glandular tissue

A

Rhodamine B

27
Q

a nuclear stain
produces red nuclei, and is used primarily as
a counterstain

A

Safranin

28
Q

Safranin gives a _____ color to collagen

A

yellow

29
Q

used in 10% aqueous solution to prepare various dilutions

A

Silver Nitrate

30
Q

Silver nitrate is used in identifying

A

spirochetes
reticulum
other fiber stains

31
Q

a nuclear stain for fixed tissues

A

toluidine blue

32
Q

toluidine blue is used as a substitute for _____ in fresh frozen tissue sections

A

thionine

33
Q

toluidine blue is recommended for staining of

A

Nissl granules
chromophilic bodies

34
Q

Van Gieson binds to collagen in the extracellular matrix, staining it

A

pink

35
Q

Van Gieson is often combined with a stain for elastic fibers (elastic van Gieson) which stain _____

A

black

36
Q

used for demonstration of neuroglia in frozen
sections

A

victoria blue

37
Q

a silver reduction method that demonstrates
phosphates and carbonates, but these are
usually present along with calcium

A

Von Kossa stain

38
Q

causes blood cells to exhibit four major staining properties that allow the cell types to be distinguished

A

wright stain

39
Q

affinity for methylene blue
color product:

A

basophilia
very dark bluish purple

40
Q

affinity for the oxidation products of methylene blue called azures, which are reddish purple

A

azurophilia

41
Q

affinity for eosin
color product:

A

acidophilia
red to red-orange

42
Q

affinity for a complex of dyes in the mixture, which are reddish brown to pale lilac

A

neutrophilia

MTAP 2 - HISTOPATH - FINALS EXAM - ADDTL READINGS (18 decks)

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