Methylene Blue Flashcards
common basic nuclear stain
methylene blue
methylene blue usually contains some
azure
methyl violet
can be substituted for
Janus Green B stain
Carmine Stain
involves the oxidation of methylene blue, resulting in loss of methyl groups and leaving lower homologues of the dye (azures) and deaminized oxidation products (thiazoles)
polychroming
resulting mixture of methylene blue, azures and thiazoles
polychrome methylene blue
methylene blue stains nuclei
blue
methylene blue stains cartilage matrix, mucin, mast cell granules and connective tissues
reddish-violet color
valuable stain for plasma cells & may also be employed in cytological examinations of fresh sputum for malignant cells, as a bacterial stain for evaluation and differentiation of bacterial organisms, for diagnosis of diphtheria, and for
vital staining of the nervous tissue
polychrome methylene blue:
originally known as Eosin- Methylene Blue (EMB) method
Mallory’s Phloxine Methylene Blue Stain
metachromatic dye formed whenever methylene blue is heated in fixed alkali or alkali carbonate
methylene violet
a lipophilic stain; it will accumulate in
lipid globules inside cells, staining them red.
Nile Red
a dye that is more soluble in fat than in water
or alcohols -> stain for neutral lipids
Oil Red O
an excellent stain for elastic fibers, and is especially recommended in dermatological studies
Orcein
a selective stain for unsaturated lipids and for
lipoproteins such as myelin
Osmium Tetroxide
Osmium tetroxide stains
black
an all-around useful stain for many things
Periodic Acid Schiff
The aldehydes are then detected by the Schiff
reagent, which stains them
reddish purple
a common negative stain for viruses, nerves,
polysaccharides, and other biological tissue
materials
phosphotungstic acid
phosphotungstic acid is an ideal stain for the demonstration of striated muscle fibers and mitochondria, which stain
blue
employed as a contrast stain to acid fuchsin, for the demonstration of connective tissue (Van Gieson’s stain)
picric acid
picric acid is a cytoplasmic stain in contrast to
basic dyes
picric acid is a as a counterstain to
crystal violet
T/F. picric acid is a tissue fixative, and as a decalcifying agent
true
an insoluble colored salt of ferric ferrocyanide (an iron cyanide compound) normally utilized for the manufacture of paints
prussian blue
Prussian blue is used for microanatomical color contrast of specimens and for demonstration of the _____ and _____ by injection (intravital staining).
blood
lymph vessels
used with osmic acid to fix and stain blood and
glandular tissue
Rhodamine B
a nuclear stain
produces red nuclei, and is used primarily as
a counterstain
Safranin
Safranin gives a _____ color to collagen
yellow
used in 10% aqueous solution to prepare various dilutions
Silver Nitrate
Silver nitrate is used in identifying
spirochetes
reticulum
other fiber stains
a nuclear stain for fixed tissues
toluidine blue
toluidine blue is used as a substitute for _____ in fresh frozen tissue sections
thionine
toluidine blue is recommended for staining of
Nissl granules
chromophilic bodies
Van Gieson binds to collagen in the extracellular matrix, staining it
pink
Van Gieson is often combined with a stain for elastic fibers (elastic van Gieson) which stain _____
black
used for demonstration of neuroglia in frozen
sections
victoria blue
a silver reduction method that demonstrates
phosphates and carbonates, but these are
usually present along with calcium
Von Kossa stain
causes blood cells to exhibit four major staining properties that allow the cell types to be distinguished
wright stain
affinity for methylene blue
color product:
basophilia
very dark bluish purple
affinity for the oxidation products of methylene blue called azures, which are reddish purple
azurophilia
affinity for eosin
color product:
acidophilia
red to red-orange
affinity for a complex of dyes in the mixture, which are reddish brown to pale lilac
neutrophilia
