Iron Hematoxylin Solutions

Question 1

used only for

Answer 1

differential staining
regressive staining

Question 2

using _______ as a differentiating agent

Answer 2

acid-alcohol

Question 3

Two main iron hematoxylin solutions for routine work in the laboratory:

Answer 3

Weigert’s
Heidenhain’s

Question 4

ferric ammonium chloride

Answer 4

Weigert’s

Question 5

ferric ammonium sulfate (iron alum)

Answer 5

Heidenhain’s

Question 6

Dye lake when ferric salts used as mordants

Answer 6

intense blue-black

Question 7

ripen hematoxylin rapidly & are active oxidizing agents -> do not keep well as a prepared mixture

Answer 7

Ferric salts

Question 8

insoluble lake gradually precipitates out, so that premixed stains are not very stable

Answer 8

hematoxylin and ferric salts

Question 9

Type of Iron Hematoxylin stain used for Mitochondria

Answer 9

Regaud’s hematoxylin

Question 10

Regaud’s hematoxylin is used for tissues fixed in

Answer 10

Potassium dichromate
Formalin

Question 11

Disadvantage of Regaud’s hematoxylin

Answer 11

Result are not uniform (over stained and under stained)

Question 12

standard iron hematoxylin stain used in the laboratory

Answer 12

Weigert’s hematoxylin

Question 13

Weigert’s hematoxylin demonstrates

Answer 13

muscle fibers
connective tissues

Question 14

a popular cytological stain, especially for the study of mitosis

Answer 14

Heidenhain’s hematoxylin

Question 15

Heidenhain’s hematoxylin chromatin material

Answer 15

blue black

Question 16

T/F. Heidenhain’s cytological stain recommended for regressive staining of thin sections

Answer 16

true