Studying Cells And Tissues W2

Question 1

Under normal EM conditions what do you expect to find in the nucleus (6)

Answer 1

Nuclear membrane 
Nuclear pores 
Nucleolus
Heterochromatin 
Euchromatin 
Chromosomes

Question 2

Under EM what colour is euchromatin

Answer 2

Light grey

Question 3

Under EM what colour is heterochromatin

Answer 3

Dark grey

Question 4

Why do we have nuclear pores

Answer 4

To allow mRNA to leave the nucleus

Question 5

What is the space inside the endoplasmic reticulum called

Answer 5

Cistern

Question 6

Where do metabolic processes occur with reference to the endoplasmic reticulum

Answer 6

On the surface and within the cistern

Question 7

Give examples of metabolic processes that occur within/on surface of endoplasmic reticulum (4)

Answer 7

Protein synthesis and modification

Lipid and steroid synthesis

Detoxification

Manufacture of cellular membranes

Question 8

Where are ribosomes manufactures

Answer 8

In the nucleolus

Question 9

Do ribosomes have to be attached to the ER

Answer 9

No

Question 10

What are ribosomes (and therefore rER) composed of

Answer 10

Ribonucleic acid

Question 11

If ribosomes (and therefore rER) composed of ribonucleic acid what stain do they attract

Answer 11

Basic stain - dark purple

Question 12

What colour does protein stain under a LM

Answer 12

Pink

Question 13

What do cells that produce protein for export into a duct look like (2regions)

Answer 13

Have a basophilic region where most of the rER is and then acidophilus regions on the inside where the secretory vesicles with the protein are

Question 14

Ribosomes attached to the endoplasmic reticulum are involved in ……… ……..

Answer 14

Protein synthesis

Question 15

How is the ER involved in protein synthesis ?

Answer 15

At the rER proteins are packaged I nto vesicles for export

Question 16

Where can protein synthesis occur

Answer 16

rER

Cytosol

Question 17

What can the rER do to accomodate for accumulating proteins

Answer 17

It expands

Question 18

Smooth ER is primarily found in cells involved in….(3)…production

Answer 18

Steroid

Cholesterol

Triglyceride

Question 19

Smooth ER is also found in cells that function to …….. ……. …….eg (2)

Answer 19

Detoxify toxic material

Eg alcohol and barbiturates

Question 20

Examples of cells that would have smooth ER present (1) that are present within (3) organs

Answer 20

Hepatocytes

Present in
Testis
Ovaries
Adrenal gland

Question 21

The Golgi complex is is an area of (2)

Of substances manufactured by the (2)

Answer 21

Packaging and modification

Smooth ER
Rough ER

Question 22

Golgi body has two faces what are the names

Answer 22

Cis and trams face

Question 23

Name of Golgi complex face, facing ER

Answer 23

Cis

Question 24

Name of Golgi complex face, facing away from ER

Answer 24

Trans face

Question 25

Which face of Golgi complex receives becomes from ER

Answer 25

Cis

Question 26

Which face of Golgi complex releases vesicles

Answer 26

Trans

Question 27

What happens to vesicles that come out of trans face of Golgi complex

Answer 27

Either go to membrane Or out of the cell

Question 28

Anything manufactured on the rER goes where

Answer 28

Outside of the cell or to cell membrane Things that stay in the cell are made by free ribosomes floating around in the cell

Question 29

Are lysosomes acidic or basic

Answer 29

Acidic

Question 30

What enzymes do lysosomes contain

Answer 30

Hydrolytic

Question 31

What are perixisomes

Answer 31

Self replacing organelles that contain oxidative enzymes

Question 32

What do peroxisomes do

Answer 32

Involved in catabolism of long fatty acid chains

Question 33

Average lifespan of mitochondria

Answer 33

10 Days

Question 34

What are cristae

Answer 34

I foldings of inner mitochondrial membrane

Question 35

whats the name of the dots seen inside mitochondria

Answer 35

matrix granules

Question 36

2 types of cristae (ie their look)

Answer 36

shelf like cristae tubular cristae (under em look like small circles)

Question 37

mitochondria with tubular cristae are most often found in mitochondria in cells that produce......

Answer 37

steroids

Question 38

mitochondria with shelf like cristae are found in mitochondria in cells that produce .....

Answer 38

protein

Question 39

Example of organs which contain cells that may have mitochondria with tubular cristae (3)

Answer 39

testes ovaries and adrenal glands

Question 40

can the cytoskeleton be seen using a LM

Answer 40

not unless special staining is used

Question 41

much of the general eosenophilia of the cytoplasm of a cell may be due to the .....

Answer 41

cytoskelton

Question 42

what does the cytoskeleton look like under an EM

Answer 42

cant really see it, but the grainy nature of cytosol is probably elements of the sytoskeleton