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Histology > Intro To Histology W1 > Flashcards

Intro To Histology W1 Flashcards

1
Q

What does euchromatin look like under EM

A

Light grey

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2
Q

What does heterochromatin look like under EM

A

Dark grey

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3
Q

Diameter of RBC

A

7.5 um

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4
Q

How many different types of cells does a human have

A

200

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5
Q

What is autolysis

A

Cell self destruction

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6
Q

How does autolysis occur

A

Intracellular enzymes break Down proteins and leads to cell liquefaction

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7
Q

What slows autolysis

A

Lower temperatures

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8
Q

What temperature is autolysis sped up

A

30

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9
Q

At what temperature is autolysis inhibited

A

50

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10
Q

Objective of fixation

A

To preserve cells and tissues as close to natural state as possible

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11
Q

Three types of fixation (last one has two methods)

A

Chemical fixation Microwave fixation Temperature fixation -frozen sections -Cyro-microscopy

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12
Q

GENERAL Procedure steps for preparing cells and tissues for BOTH light/EM (6)

A

Fixation Processing Embedding Sectioning Staining Viewing

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13
Q

LM procedure : FIXATION

A

Primary fixation Forma aldehyde

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14
Q

LM procedure : PROCESSING

A

Dehydration and clearing

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15
Q

LM procedure: EMBEDDING

A

Paraffin wax

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16
Q

LM procedure: SECTIONING

A

5-7 um

17
Q

LM procedure: STAINING

A

H & E

18
Q

LM procedure: VIEWING

A

Varied but generally directly

19
Q

EM procedure: FIXATION

A

Primary fixation then secondary fixation with OSO4

20
Q

EM procedure: PROCESSING

A

Dehydration and clearing

21
Q

EM procedure: EMBEDDING

A

Plastic or acrylic resin

22
Q

EM procedure: SECTIONING

A

70-90 nm

23
Q

EM procedure: staining

A

Heavy metals

24
Q

EM procedure: VIEWING

A

Varied but never directly

25
Q

4 types of tissue

A

Epithelial Muscle Connective Nervous

26
Q

What is stroma

A

Structural/ non functional part of the tissue

27
Q

Parenchyma

A

Functional part of tissue

28
Q
A

lobule

Histology (11 decks)

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