STREPTOCOCCUS | IDENTIFICATION OF STAPHYLOCOCCUS & STREPTOCOCCUS Flashcards

1
Q

susceptible to bacitracin

A

S. pyogenes

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2
Q

[bacitracin susceptibility test (taxo “A” disk)]

identify the bacteria based on the result:

result:
- bacitracin susceptibility test POSITIVE

A

Streptococcus pyogenes

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3
Q

[determine whether the result is SUSCEPTIBLE or RESISTANT to novobiocin test]

result:
<17 mm zone of inhibition

A

resistant

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4
Q

[bacitracin susceptibility test (taxo “A” disk)]

determine whether the result is SUSCEPTIBLE or RESISTANT:

  • uniform growth right up to the rim of the disk
A

resistant

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5
Q

[determine the bacteria]

catalase-negative, gram-positive cocci&raquo_space; bacitracin (resistant)&raquo_space; CAMP (positive) = ???

A

streptococcus agalactiae

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6
Q

no hemolysis or α-hemolysis (greening) on sheep blood agar and smell like butterscotch, especially on chocolate agar

A

Viridans streptococci

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7
Q

group B streptococci

A

Streptococcus agalactiae

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8
Q

catalase negative

A

streptococcus

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9
Q

β-hemolytic and produce zones of hemolysis that are only slightly larger than the colonies (1-2 mm in diameter)

A

Streptococcus agalactiae

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10
Q

a wider zone of β hemolysis

A

viridans group

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11
Q

part of the normal vaginal flora in 5-25% of women

A

Streptococcus agalactiae

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12
Q

[determine the test]

procedure:
1. To 0.5 mL of undiluted rabbit plasma, add one loopful of growth from18-to 24-hour agar culture, 0.1 mL of broth culture, or a single colony from a blood agar plate

  1. Incubate in a water bath at 35 C and examine for clotting at 30-minute intervals for 4 hours
  2. If no clot is observed at the end of 4 hours, let stand at room temperature for 18 to 24 hours. Observe for clotting
A

tube coagulase test

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13
Q

[determine the test]

detects the presence of free coagulase enzyme produced by S. aureus

A

tube coagulase test

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14
Q
  • hydrolyze sodium hippurate and give a positive response in the so-called CAMP test
  • bacitracin resistant

(Christie, Atkins, Munch-Peterson/CAMP)

A

Streptococcus agalactiae

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15
Q

The Streptococcaceae consist of a large family of medically important species, including ______________ and ________________

A

Streptococcus spp., Enterococcus spp.

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16
Q

[determine the test]

Positive hydrolysis: purple color

A

rapid hippurate hydrolysis test

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17
Q

Many selective media, such as phenylethyl alcohol agar (PEA), Mannitol Salt agar (MSA), and colistin-nalidixic acid agar (CNA), are used for the isolation of ______________ from mixed cultures

A

staphylococci

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18
Q

clinically isolated streptococci is separated into ______________ and __________________

A

β-hemolytic streptococci / pyogenic (pus-forming) streptococci,

non-β-hemolytic (nonpyogenic)

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19
Q

S. pneumoniae is a member of the ___________

A

S. mitis group

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20
Q

[determine the test]

procedure:
1. Inoculate one to two colonies on a bile esculin agar plate, slant, or broth

  1. Incubate at 35 C for 18 to 24 hours
A

bile esculin test

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21
Q

[CAMP test]

a positive test is recognized by the appearance of an ___________ shape at the juncture of the ___________ and _________ streaks

A

arrowhead, S. agalactiae and S. aureus

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22
Q

[Enterococcus spp.]

_________ and _________ are the pathogenic species most commonly encountered

A

E. faecalis, E. faecium

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23
Q

[detection of GBS in pregnant women]

the most useful tests are _____________ and __________

A

positive hippurate hydrolysis, CAMP tests

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24
Q

[choose the correct answer]

CAMP test positive

a. S. pyogenes
b. S. agalactiae

A

b. S. agalactiae

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25
Q

[infections | viridans streptococci]

has a high correlation with gastrointestinal carcinoma

A

S. gallolyticus subsp. gallolyticus

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26
Q

[infections | viridans streptococci]

  • pharyngitis
  • normally found in the oral cavity, gastrointestinal tract, and female genital tract
  • transient normal microbiota of the skin
  • most common isolates associated with bacterial endocarditis in native valves and, less frequently in prosthetic valve infections
A

S. constellatus subsp. pharyngis

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27
Q

[determine the test]

procedure:
1. Emulsify a well-isolated colony in a drop of water on a glass slide to produce a dense, uniform suspension.

  1. Add one drop of plasma to the suspension and mix; then rotate for 5 seconds
A

slide coagulase test

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28
Q

Beta-Hemolytic streptococci:

A
  • S. pyogenes
  • S. agalactiae
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29
Q

group C streptococci

A

rhamnose-N-acetylgalactosamine

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30
Q

[virulence factors]

S. pyogenes can cause red red-spreading rash called

A

scarlet fever

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31
Q

they are rarely found in human disease states but produce normal coagulation (“souring”) of milk

A

group N streptococci

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32
Q

group F streptococci

A

glucopyranosyl-N-acetylgalactosamine

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33
Q

[choose the correct answer]

PYR positive

a. S. pyogenes
b. S. agalactiae

A

a. S. pyogenes

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34
Q

[determine the test]

procedure:
1. Inoculate one to two colonies into 2 mL of 6.5% NaCl broth

  1. Incubate tube at 35 C for 18 to 24 hours
A

salt tolerance test

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35
Q

it is recommended that all pregnant women be screened for GBS at ________________ gestation

A

35 to 37 weeks

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36
Q

[virulence factors]

capable of producing an erythematous sandpaper-like rash

A
  • streptolysin O
  • streptolysin S
  • deoxyribonuclease (DNase)
  • streptokinase
  • hyaluronidase
  • erythrogenic exotoxins
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37
Q

[virulence factors]

adhesion molecules that mediate attachment to host epithelial cells

A

lipoteichoic acid & protein F

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38
Q

[determine the bacteria]

no hemolysis on blood agar&raquo_space; bile esculin&raquo_space; 6.5% NaCl (positive) = ???

A

Enterococcus spp.

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39
Q

[virulence factors]

responsible for hemolysis on SBA plates incubated anaerobically

A

Streptolysin O

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40
Q

[determine the bacteria]

α-hemolysis on sheep blood agar&raquo_space; optochin disk&raquo_space; resistant&raquo_space; bile esculin (negative) = ???

A

Viridans streptococci

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41
Q

[determine the bacteria]

α-hemolysis on sheep blood agar&raquo_space; optochin disk&raquo_space; susceptible = ???

A

S. pneumoniae

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42
Q

[virulence factors]

most common DNase

A

B

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43
Q

clinical manifestations include:
- UTI
- bacteremia
- endocarditis
- intraabdominal, pelvic, wound, and soft tissue infections

A

Enterococcus spp.

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44
Q

_____________ contains the C polysaccharide unrelated to the Lancefield grouping

A

S. pneumoniae

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45
Q

[virulence factors]

_______ is acid-labile and heat-labile

A

T substance

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46
Q

Pyogenic streptococci isolated frequently from humans

A

S. pyogenes
S. agalactiae
S. dysgalactiae subsp. equisimilis
S. anginosus group

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47
Q

[virulence factors]

four (4) enzymes

A
  • streptokinase
  • streptodornase
  • hyaluronidase
  • diphosphopyridine nucleotidase
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48
Q

is immunogenic, capable of lysing the same cells and cultured cells, broken down by oxygen, and will produce hemolysis only in the absence of room air

A

Streptolysin O

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49
Q

[determine the test]

  • reacts with hydrogen peroxide to liberate oxygen
  • when a colony is emulsified in 3% hydrogen peroxide, the resultant bubbles on the slide may be visualized, which constitutes a positive test result
A

catalase test

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50
Q

[CAMP tests]

The _______________ intensifies the lysis of sheep red blood cells when placed in the appropriate inoculation points with S. aureus

A

CAMP factor

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51
Q

[determine the test]

Positive: Indicator changes from green to yellow

A

pyruvate broth

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52
Q

“flesh-eating bacteria”

A

S. pyogenes

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53
Q

the PYR and hippurate or CAMP tests can be used to identify __________ and ____________, respectively

A

groups A and B streptococci

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54
Q

[choose the correct answer]

CAMP test negative

a. S. pyogenes
b. S. agalactiae

A

a. S. pyogenes

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55
Q

normal microbiota: skin, nasopharynx, gastrointestinal tract, genital tract

A

group C, F, and G beta-hemolytic streptococci

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56
Q

[hemolysis]

sometimes called gamma hemolysis

A

non-hemolytic

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57
Q

S. saprophyticus is resistant to __________

A

novobiocin disk

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58
Q

S, agalactiae is able to hydrolyze _________ and is positive in the ___________

A

hippurate, CAMP test

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59
Q

[determine the test]

based on the differential susceptibility to ethyl hydroxycupreine hydrochloride

A

optochin susceptibility test (taxo “P” disk)

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60
Q

___________ infections usually are associated with neonates and are acquired before or during the birthing process

A

Group B S. agalactiae (GBS)

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61
Q

for isolating group A streptococci from throat swabs, the most common medium is ____________ supplemented with trimethoprim-sulfamethoxazole

A

5% sheep blood agar

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62
Q

_________ resistance is seen more frequently with E. faecium with E. faecalis

A

vancomycin

63
Q

[Hemolysis]

complete disruption of erythrocytes with clearing of the blood around the bacterial growth

A

β hemolysis

64
Q

[choose the correct answer]

bacitracin sensitive

a. S. pyogenes
b. S. agalactiae

A

a. S. pyogenes

65
Q

growth of Streptococcus is enriched on _________________

A

media containing blood or serum

66
Q

[determine the test]

positive result: causes clumping when mixed with plasma

A

slide coagulase test

67
Q

[determine the test]

Positive test result—blackening of the agar or broth

A

bile esculin test

68
Q

[optochin susceptibility test (taxo “P” disk)]

identify the bacteria based on expected results:

  • Zone diameter of inhibition >15 mm
A

S. pneumoniae

69
Q

[determine the test]

This test is used to determine the ability of an organism to utilize pyruvate. This aids in the differentiation

A

pyruvate broth

70
Q

other name for pneumoniae

A

pneumococcus

71
Q

colonies: dome-shaped appearance

A

S. pneumoniae

72
Q

normal microbiota: oral cavity

A

Abiotrophia spp.

73
Q

[virulence factors]

scarlet fever is caused by

A

streptococcal pyrogenic exotoxins

74
Q

[determine the test]

  • releases free β-naphthylamide, which is detected and shown by the color change after adding the color reagent
  • uses filter paper disk
A

PYR test

75
Q

[determine the test]

A modified broth containing 6.5% NaCl is used

A

salt tolerance test

76
Q

[hemolysis]

incomplete lysis of erythrocytes with reduction of hemoglobin and the formation of green pigment

A

α hemolysis

77
Q

group B streptococci

A

rhamnose-glucosamine polysaccharide

78
Q

[determine the test]

uses 10% sodium desoxycholate e to the side of a young (13- to 24-hour), well-isolated colony growing on 5% sheep blood agar

A

bile solubility test

79
Q

the cell wall of S. pneumoniae contains an antigen, referred to as _____________

A

C substance

80
Q

[Lancefield classification]

group A streptococci

A

rhamnose-N-acetylglucosamine

81
Q

[Lancefield classification]

this carbohydrate is contained in the cell wall of many streptococci and forms the basis of serologic grouping into Lancefield groups ______ and ______

A

A-H, K-U

82
Q

[StrepB Carrot Broth (SCB)]

vaginal or rectal swabs are placed into SCB and incubated ____ to ____ hours at ______

A

18 to 24, 35°C

83
Q

[infections | viridans streptococci]

  • normal oral and gastrointestinal microbiota
  • associated with abscess formation in the oropharynx, brain, and peritoneal cavity
A

S. anginosus group

84
Q

[virulence factors]

infections:

“strep throat”

A

pharyngitis

85
Q

the CAMP test detects production of a diffusible, extracellular protein that __________ the hemolysis of sheep erythrocytes by ______________

A

enhances, Staphylococcus aureus

86
Q

[virulence factors]

obtained from streptococci by proteolytic digestion, which rapidly destroys M proteins

A

T substance

87
Q

[Lancefield classification]

the serologic specificity of the group-specific carbohydrate is determined by ___________

A

amino sugar

88
Q

most laboratories identify Enterococcus spp. presumptively by demonstrating that the isolate is ______ and ______ positive and that it grows at ______ and in _______

A

PYR and LAP, 45°C, 6.5% NaCl

89
Q

two (2) important tests for the laboratory diagnosis of S. pneumoniae

A
  • optochin susceptibility test
  • bile solubility test
90
Q

[StrepB Carrot Broth (SCB)]

β-hemolytic GBS produce an ________ or ____ pigment in SCB after incubation for _____ hours

A

orange or red, six (6)

91
Q

PYR-positive

A

S. pyogenes

92
Q

[dtermine the test]

produces an extracellular enzyme, “CAMP” factor

A

CAMP test (group B streptococcus)

93
Q

_____________ determines the lysis of S. pneumoniae in the presence of bile salts

A

bile solubility test

94
Q

[virulence factors]

four (4) DNases

A

A, B, C, D

95
Q

normal microbiota: oral cavity, gastrointestinal tract, female genital tract

A

Viridans streptococci

96
Q

[bacitracin susceptibility test (taxo “A” disk)]

identify the bacteria based on the result:

result:
- bacitracin susceptibility test NEGATIVE

A

Streptococcus agalactiae

97
Q

[virulence factors]

infections:

  • multisystem involvement that includes renal and respiratory failure, rash, and diarrhea
  • another disease mediated by production of potent streptococcal pyrogenic exotoxins (SpeA)
A

streptococcal toxic shock syndrome

98
Q

[determine the test]

Subsequent addition of triketohydrindene hydrate (ninhydrin) results in the oxidative deamination of the αamino group in glycine

A

rapid hippurate hydrolysis test

99
Q
  • normal microbiota: humans, animals, and birds
  • colonizers
A

Enterococcus spp.

100
Q

[bile esculin test]

____________, which is the byproduct of esculin hydrolysis, combined with ______________ in the medium to give a black complex

A

esculetin, ferric citrate

101
Q

[dtermine the test]

positive result: forms a clot when incubated with plasma

A

tube coagulase test

102
Q

a brown halo around colonies on bile esculin agar and a positive PYR reaction is indicative of _____________

A

Enterococcus spp.

103
Q

[virulence factors]

enzymes:

  • related to the organism’s ability to kill leukocytes
A

diphosphopyridine nucleotidase

104
Q

[virulence factors]

infections:

  • occurs in association with streptococcal pharyngitis
  • manifested by a rash of the face and upper trunk
A

scarlet fever

105
Q

[infections | viridans streptococci]

  • bacteremia
  • septicemia
  • endocarditis
A

S. bovis group

106
Q

streptococcus is arranged in _______ or ________

A

pairs, chains

107
Q

facultative anaerobes

A

Streptococcus

108
Q

[virulence factors]

enzymes:

  • depolymerizes DNA
  • decrease viscosity of DNA solutions
  • liquefy exudates and facilitate removal of pus and necrotic tissue
A

streptodornase

109
Q

[detection of GBS in pregnant women]

___________, which can be substituted for Lim or TransVag broth

A

StrepB Carrot Broth (SCB)

110
Q

________ and ________ are normal biota of the human gastrointestinal tract and female genitourinary tract

A

E. faecalis, E. faecium

111
Q

[determine whether the result is SUSCEPTIBLE or RESISTANT to novobiocin test]

result:
>17 mm zone of inhibition

A

susceptible

112
Q

[determine the test]

Positive: Colony disintegrates; an imprint of the lysed colony may remain within the zone

A

bile solubility test

113
Q

S. pneumoniae is susceptible to ___________

A

optochin

114
Q

group D streptococci

A

glycerol teichoic acid containing D-alanine and glucose

115
Q

[capsular polysaccharide]

the capsule is antigenic and can be identified with appropriate antisera in the ____________

A

Neufield test

116
Q

[virulence factors]

________ to determine whether an individual has had a recent infection with S. pyogenes

A

antistreptolysin (ASO)

117
Q

these streptococci occur primarily in animals

A

group E, F, G, H, & K-U streptococci

118
Q

[determine the test]

produces an “arrow-head” hemolysis

A

CAMP test (group B streptococcus)

119
Q

Other CoNS that are resistant to 5-μg novobiocin are ________, __________, and _________

A

Staphylococcus xylosus, Staphylococcus cohnii, Staphylococcus sciuri

120
Q

[determine the test]

Positive result: dark pink to red color

A

PYR test

121
Q

an oxygen-stable, nonimmunogenic hemolysin capable of lysing erythrocytes, leukocytes, and platelets in the presence of room air

A

Streptolysin S

122
Q
  • normal microbiota: female genital tract and lower gastrointestinal tract
  • occasional colonizer of the upper respiratory tract
A

Streptococcus agalactiae (group B)

123
Q
  • not highly invasive
  • they enter tissue during dental or surgical procedures, which could lead to tooth abscesses, abdominal infections, bacteremia, or valve endocarditis and late-onset prosthetic valve endocarditis
A

Viridans streptococci

124
Q

any negative slide coagulase test result must be confirmed with the _______________

A

tube coagulase test

125
Q

[virulence factors]

is weakly immunogenic

A

hyaluronic acid capsule

126
Q

[virulence factors]

infections:

  • “flesh-eating bacteria”
  • infection tends to disseminate into deeper tissues
  • categorized as type 1, 2, or 3
A

necrotizing fasciitis

127
Q

[bacitracin susceptibility test (taxo “A” disk)]

determine whether the result is SUSCEPTIBLE or RESISTANT:

  • any zone of inhibition around the bacitracin disk
A

susceptible

128
Q

causes septicemia, pneumonia, and meningitis in newborns (first 5 days of life)

A

Group B S. agalactiae (GBS)

129
Q

[optochin susceptibility test (taxo “P” disk)]

identify the bacteria based on expected results:

  • Zone diameter of inhibition <15 mm
A

viridans streptococci group

130
Q

[choose the correct answer]

PYR negative

a. S. pyogenes
b. S. agalactiae

A

b. S. agalactiae

131
Q

[virulence factors]

infections:

three (3) pyodermal infections

A
  • impetigo
  • erysipelas
  • cellulitis
132
Q

[virulence factors]

enzymes:

  • splits hyaluronic acid
  • aids in spreading infecting microorganisms
  • spreading factor
A

hyaluronidase

133
Q

_________ is the only species of beta-hemolytic streptococci that will give a positive PYR reaction

A

S. pyogenes

134
Q

___________ plays a key role in the development of dental caries

A

S. mutans

135
Q

[virulence factors]

infections:

  • post-streptococcal sequelae
  • edema, hypertension, hematuria, and proteinuria, can follow respiratory tract or cutaneous infections and is mediated by antigen-antibody complexes that deposit in glomeruli
A

acute glomerulonephritis

136
Q

primary cause of bacterial pneumonia, meningitis, and otitis media

A

Streptococcus pneumoniae

137
Q

[identify the bacteria spp.]

The colonies are round; are at least 1 to 2 mm in diameter; are convex, opaque, and glistening with a complete edge; and are soft or butter-like in consistency.

Some varieties are surrounded by zones of beta-hemolysis.

The color of the colony varies from an off-white to cream color to golden yellow

A

Staphylococcus spp.

138
Q

[virulence factors]

resist phagocytosis and adheres bacterial cell to mucosal cells

A

M protein

139
Q

_________ can cause skin infections of dogs but uncommonly infects humans

A

S. canis

140
Q

[virulence factors]

permits differentiation of certain types of streptococci by agglutination with specific antisera

A

T substance

141
Q

[StrepB Carrot Broth (SCB)]

broths that turn orange or red are reported as ________ for GBS

A

positive

142
Q

[hemolysis]

colonies are surrounded by a very small zone of no hemolysis

A

Alpha-prime (α’) or wide zone hemolysis

143
Q

some species of Streptococcus are ___________

A

capnophilic

144
Q

[virulence factors]

infections:

  • post-streptococcal sequelae
  • manifested by fever, carditis, subcutaneous nodules, and polyarthritis, usually follow respiratory tract infections
  • after strep throat
A

rheumatic fever

145
Q

[infections | viridans streptococci]

  • the most commonly isolated among the viridans streptococci
  • primary contributor to dental caries
  • bacteremia
A

S. mutans

146
Q

[determine the bacteria]

α-hemolysis on sheep blood agar&raquo_space; optochin disk&raquo_space; resistant&raquo_space; bile esculin&raquo_space; 6.5% NaCl (PYR) positive = ???

A

Enterococcus spp.

147
Q

colonizer of nasopharynx

A

Streptococcus pneumoniae

148
Q

[virulence factors]

attachment of streptococci to the oral mucosal cells

A

fibronectin binding

149
Q
  • not considered normal microbiota
  • inhabits skin and upper respiratory tract of humans
  • carried on nasal, pharyngeal, and sometimes anal mucosa
A

Streptococcus pyogenes (group A)

150
Q

[determine the test]

Positive test result: visible turbidity in broth

A

salt tolerance test

151
Q

[virulence factors]

enzymes:

  • transforms the plasminogen of human plasma into plasmin
  • treatment of pulmonary emboli, coronary artery, and venous thromboses
  • used to lyse clots
A

streptokinase

152
Q
  • commonly colonize the gastrointestinal tract
  • they can be isolated from the oropharynx, female genital tract, and skin
  • causes UTI
A

Enterococcus spp.

153
Q

[choose the correct answer]

bacitracin resistant

a. S. pyogenes
b. S. agalactiae

A

b. S. agalactiae

154
Q

[determine the bacteria]

catalase-negative, gram-positive cocci&raquo_space; bacitracin (susceptible) = ???

A

streptococcus pyogenes