STREPTOCOCCUS | IDENTIFICATION OF STAPHYLOCOCCUS & STREPTOCOCCUS Flashcards
susceptible to bacitracin
S. pyogenes
[bacitracin susceptibility test (taxo “A” disk)]
identify the bacteria based on the result:
result:
- bacitracin susceptibility test POSITIVE
Streptococcus pyogenes
[determine whether the result is SUSCEPTIBLE or RESISTANT to novobiocin test]
result:
<17 mm zone of inhibition
resistant
[bacitracin susceptibility test (taxo “A” disk)]
determine whether the result is SUSCEPTIBLE or RESISTANT:
- uniform growth right up to the rim of the disk
resistant
[determine the bacteria]
catalase-negative, gram-positive cocci»_space; bacitracin (resistant)»_space; CAMP (positive) = ???
streptococcus agalactiae
no hemolysis or α-hemolysis (greening) on sheep blood agar and smell like butterscotch, especially on chocolate agar
Viridans streptococci
group B streptococci
Streptococcus agalactiae
catalase negative
streptococcus
β-hemolytic and produce zones of hemolysis that are only slightly larger than the colonies (1-2 mm in diameter)
Streptococcus agalactiae
a wider zone of β hemolysis
viridans group
part of the normal vaginal flora in 5-25% of women
Streptococcus agalactiae
[determine the test]
procedure:
1. To 0.5 mL of undiluted rabbit plasma, add one loopful of growth from18-to 24-hour agar culture, 0.1 mL of broth culture, or a single colony from a blood agar plate
- Incubate in a water bath at 35 C and examine for clotting at 30-minute intervals for 4 hours
- If no clot is observed at the end of 4 hours, let stand at room temperature for 18 to 24 hours. Observe for clotting
tube coagulase test
[determine the test]
detects the presence of free coagulase enzyme produced by S. aureus
tube coagulase test
- hydrolyze sodium hippurate and give a positive response in the so-called CAMP test
- bacitracin resistant
(Christie, Atkins, Munch-Peterson/CAMP)
Streptococcus agalactiae
The Streptococcaceae consist of a large family of medically important species, including ______________ and ________________
Streptococcus spp., Enterococcus spp.
[determine the test]
Positive hydrolysis: purple color
rapid hippurate hydrolysis test
Many selective media, such as phenylethyl alcohol agar (PEA), Mannitol Salt agar (MSA), and colistin-nalidixic acid agar (CNA), are used for the isolation of ______________ from mixed cultures
staphylococci
clinically isolated streptococci is separated into ______________ and __________________
β-hemolytic streptococci / pyogenic (pus-forming) streptococci,
non-β-hemolytic (nonpyogenic)
S. pneumoniae is a member of the ___________
S. mitis group
[determine the test]
procedure:
1. Inoculate one to two colonies on a bile esculin agar plate, slant, or broth
- Incubate at 35 C for 18 to 24 hours
bile esculin test
[CAMP test]
a positive test is recognized by the appearance of an ___________ shape at the juncture of the ___________ and _________ streaks
arrowhead, S. agalactiae and S. aureus
[Enterococcus spp.]
_________ and _________ are the pathogenic species most commonly encountered
E. faecalis, E. faecium
[detection of GBS in pregnant women]
the most useful tests are _____________ and __________
positive hippurate hydrolysis, CAMP tests
[choose the correct answer]
CAMP test positive
a. S. pyogenes
b. S. agalactiae
b. S. agalactiae
[infections | viridans streptococci]
has a high correlation with gastrointestinal carcinoma
S. gallolyticus subsp. gallolyticus
[infections | viridans streptococci]
- pharyngitis
- normally found in the oral cavity, gastrointestinal tract, and female genital tract
- transient normal microbiota of the skin
- most common isolates associated with bacterial endocarditis in native valves and, less frequently in prosthetic valve infections
S. constellatus subsp. pharyngis
[determine the test]
procedure:
1. Emulsify a well-isolated colony in a drop of water on a glass slide to produce a dense, uniform suspension.
- Add one drop of plasma to the suspension and mix; then rotate for 5 seconds
slide coagulase test
Beta-Hemolytic streptococci:
- S. pyogenes
- S. agalactiae
group C streptococci
rhamnose-N-acetylgalactosamine
[virulence factors]
S. pyogenes can cause red red-spreading rash called
scarlet fever
they are rarely found in human disease states but produce normal coagulation (“souring”) of milk
group N streptococci
group F streptococci
glucopyranosyl-N-acetylgalactosamine
[choose the correct answer]
PYR positive
a. S. pyogenes
b. S. agalactiae
a. S. pyogenes
[determine the test]
procedure:
1. Inoculate one to two colonies into 2 mL of 6.5% NaCl broth
- Incubate tube at 35 C for 18 to 24 hours
salt tolerance test
it is recommended that all pregnant women be screened for GBS at ________________ gestation
35 to 37 weeks
[virulence factors]
capable of producing an erythematous sandpaper-like rash
- streptolysin O
- streptolysin S
- deoxyribonuclease (DNase)
- streptokinase
- hyaluronidase
- erythrogenic exotoxins
[virulence factors]
adhesion molecules that mediate attachment to host epithelial cells
lipoteichoic acid & protein F
[determine the bacteria]
no hemolysis on blood agar»_space; bile esculin»_space; 6.5% NaCl (positive) = ???
Enterococcus spp.
[virulence factors]
responsible for hemolysis on SBA plates incubated anaerobically
Streptolysin O
[determine the bacteria]
α-hemolysis on sheep blood agar»_space; optochin disk»_space; resistant»_space; bile esculin (negative) = ???
Viridans streptococci
[determine the bacteria]
α-hemolysis on sheep blood agar»_space; optochin disk»_space; susceptible = ???
S. pneumoniae
[virulence factors]
most common DNase
B
clinical manifestations include:
- UTI
- bacteremia
- endocarditis
- intraabdominal, pelvic, wound, and soft tissue infections
Enterococcus spp.
_____________ contains the C polysaccharide unrelated to the Lancefield grouping
S. pneumoniae
[virulence factors]
_______ is acid-labile and heat-labile
T substance
Pyogenic streptococci isolated frequently from humans
S. pyogenes
S. agalactiae
S. dysgalactiae subsp. equisimilis
S. anginosus group
[virulence factors]
four (4) enzymes
- streptokinase
- streptodornase
- hyaluronidase
- diphosphopyridine nucleotidase
is immunogenic, capable of lysing the same cells and cultured cells, broken down by oxygen, and will produce hemolysis only in the absence of room air
Streptolysin O
[determine the test]
- reacts with hydrogen peroxide to liberate oxygen
- when a colony is emulsified in 3% hydrogen peroxide, the resultant bubbles on the slide may be visualized, which constitutes a positive test result
catalase test
[CAMP tests]
The _______________ intensifies the lysis of sheep red blood cells when placed in the appropriate inoculation points with S. aureus
CAMP factor
[determine the test]
Positive: Indicator changes from green to yellow
pyruvate broth
“flesh-eating bacteria”
S. pyogenes
the PYR and hippurate or CAMP tests can be used to identify __________ and ____________, respectively
groups A and B streptococci
[choose the correct answer]
CAMP test negative
a. S. pyogenes
b. S. agalactiae
a. S. pyogenes
normal microbiota: skin, nasopharynx, gastrointestinal tract, genital tract
group C, F, and G beta-hemolytic streptococci
[hemolysis]
sometimes called gamma hemolysis
non-hemolytic
S. saprophyticus is resistant to __________
novobiocin disk
S, agalactiae is able to hydrolyze _________ and is positive in the ___________
hippurate, CAMP test
[determine the test]
based on the differential susceptibility to ethyl hydroxycupreine hydrochloride
optochin susceptibility test (taxo “P” disk)
___________ infections usually are associated with neonates and are acquired before or during the birthing process
Group B S. agalactiae (GBS)
for isolating group A streptococci from throat swabs, the most common medium is ____________ supplemented with trimethoprim-sulfamethoxazole
5% sheep blood agar