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BACTE > BACTERIA CULTURE > Flashcards

BACTERIA CULTURE Flashcards

1
Q

nutritional needs classification:

A
  • fastidious
  • nonfastidious
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2
Q

Identification schemes can be classified into one of two categories:

those based on ___________ characteristics of bacteria and those based on __________ characteristics.

A

genotypic, phenotypic

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3
Q

all bacterial cells within a single colony are the same genus and species, having identical genetic and phenotypic characteristics

A

pure colony

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4
Q

[types of culture media]

supports the growth of most nonfastidious microbes

A

nonselective media

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5
Q

[types of culture media]

___________ is selective for gram-positive organisms

A

Columbia agar with colistin nalidixic acid (CNA)

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6
Q

three (3) common backup broths

A
  • thioglycollate broth (thio)
  • brain-heart infusion broth (BHIB)
  • tryptic soy broth (TSB)
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7
Q

[solid medium]

melting at high temperatures ______ but resolidifying after the temperature fall below ______

A

95 degrees C, 50 degrees C

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8
Q

[types of culture media]

the standard nonselective medium

A

sheep blood agar

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9
Q

grow throughout the medium, because they are unaffected by the variation in oxygen content

A

facultative anaerobes & aerotolerant organisms

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10
Q

solidifying agent added to the nutrients and water

A

solid medium

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11
Q

[types of culture media]

  • contains the dye crystal violet, which inhibits gram-positive organisms
A

MacConkey agar

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12
Q

[types of culture media]

used as a supplement to agar plates to detect small numbers of most aerobes, anaerobes, and microaerophiles

A

broth media / supplemental broth

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13
Q

[types of culture media]

support the growth of one group of organisms but not another by adding antimicrobials, dyes, or alcohol to a particular medium

A

selective media

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14
Q

refers to the percentage of patients without disease that will test negative for the presence of the organism

A

specificity

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15
Q

[types of culture media]

differentiates between lactose-fermenting and nonfermenting gram-negative rods by the color of the colonial growth

A

MacConkey agar

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16
Q

[types of culture media]

_________ is a liquid medium designed to encourage the growth of small numbers of a particular organism while suppressing other flora present

A

enrichment broth

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17
Q

________ broth contains a small amount of agar, making it a semisolid medium

A

thioglycollate

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18
Q

grow slightly below the surface where oxygen concentrations are lower than atmospheric concentrations

A

microaerophilic organisms

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19
Q

some broths may contain a ________, such as _________ that may change the color in the presence of bacterial metabolites

A

pH indicator, phenol red

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20
Q

Certain schemes rely on both _________ and _________ characteristics

A

genotypic, phenotypic

21
Q

[types of culture media]

_____________ can also be differential media if, in addition to their inhibitory activity, they differentiate between groups of organisms.

A

selective media

22
Q

[types of culture media]

  • considered both a nutritive and differential medium
  • it differentiates organisms based on whether they are alpha (a)-, beta (b)-, or gamma (g) hemolytic
A

blood agar

23
Q

[types of culture media]

____________ is selective for enteric gram-negative bacilli

A

MacConkey agar

24
Q

[types of culture media]

Nutritive media can also be ___________, in that microorganisms can be distinguished on the basis of certain growth characteristics evident on the medium

A

differential

25
Q

[types of culture media]

support the growth of a wide range of microorganisms and are considered nonselective because, theoretically, the growth of most organisms is supported

A

nutritive media

26
Q

two (2) types of selective media

A
  • MacConkey agar
  • Columbia agar w/ colistin and nalidixic acid (CNA)
27
Q

nutrients are dissolved in water

A

broth media

28
Q

indicates the percentage of patients in whom the organism is present who actually test positive

A

sensitivity

29
Q

example of nutritive media

A

blood agar or chocolate agar

30
Q

stable solid gel referred to as ______

A

agar

31
Q

[types of culture media]

support the growth of one type / group of microbes but not another

A

selective media

32
Q

medium that contain both liquid and solid phase

A

biphasic medium

33
Q

an example of a supplemental broth

A

thioglycollate broth

34
Q

a characteristic odor of an organism having a fruity or grapelike smell

A

Pseudomonas aeruginosa

35
Q

the presence of a specific gene or a particular nucleic acid sequence unique to the organism is interpreted as a ______________________

A

definitive identification of the organism

36
Q

an example of an enriched medium

A

chocolate agar

37
Q

[types of culture media]

medium for gram-positive organisms, because the antimicrobial CNA inhibits gram-negative organisms

A

Columbia agar w/ colistin and nalidixic acid

38
Q

[types of culture media]

incubated for a certain period and then must be subcultured to isolate the particular organism.

A

enrichment broth

39
Q

[types of culture media]

inoculated, along with primary solid (agar) media, so small numbers of organisms present may be detected this allows detection of anaerobes in aerobic cultures and organisms that may be damaged by either previous or concurrent antimicrobial therapy

A

backup broth

40
Q

most common solidifying agent

A

agarose

41
Q

[types of culture media]

_________ may contain inhibitory substances such as antimicrobials, dyes, or alcohol

A

selective media

42
Q

[types of culture media]

contain growth enhancers that are added to nonselective agar to allow fastidious organisms to flourish

A

enriched media

43
Q

two (2) phases of growth media

A
  • broth (liquid)
  • agar (solid)
44
Q

____________ will grow at the bottom of the broth tube, whereas _________ will grow near the surface

A

strict anaerobes, aerobes

45
Q

[types of culture media]

___________ allow grouping of microbes based on different characteristics demonstrated on the medium

A

differential media

46
Q

process of growing microorganisms in culture by taking bacteria from the infection site by some means of specimen collection and growing them in the artificial environment of the laboratory

A

cultivation

47
Q

[types of culture media]

used to enhance the growth of group B streptococci

A

Lim broth (Todd Hewitt with CNA)

48
Q

_______________ involve characterization of some portion of a bacterium’s genome using molecular methods for DNA or RNA analysis

A

genotypic identification methods

49
Q

[types of culture media]

_________ also called as supplemental or enrichment broth

A

backup broth

BACTE (6 decks)

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