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Strep and Enterococcus Flashcards

1
Q

strep and entero gram stain and biochems:
aerotolerance
catalase
oxidase

A

gp pairs/chains
aerotolerant
cat neg
oxidase neg

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2
Q

strep routine media

A

BAP, CHOC, CNA, PEA

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3
Q

strep/entero habitat

A

respiratory tract, gi tract

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4
Q

alpha hemo strep sp (2)

A

pneumoniae, viridans

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5
Q

gamma hemo entero

A

faecalis, faecium

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6
Q

strep pneumo hemolysis and biochems:
optochin
bile solubility

A

alpha hemo
optochin sensitive
bile soluble

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7
Q

strep agalactiae group and biochems:
Bacitracin
optochin
hippurate hydrolysis
CAMP

A

group b
bacitracin R
optochin R
hippurate hydrolysis pos
CAMP pos

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8
Q

GAS diseases

A

impetigo
rheumatic fever
scarlet fever
necrotizing fasciitis
glomerulonephritis

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9
Q

M protein

A

adherence, antiphagocytic, coag activation

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10
Q

Streptolysin S

A

GAS hemolysis aero/anaero

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11
Q

streptolysin O

A

GAS hemolysis anaerobic

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12
Q

GAS toxins (5)

A

SpeA/B/C super ag
streptokinase
DNAse
hyaluronidase
plasminogen-plasmin binding

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13
Q

Streptokinase

A

lyse fibrin clots by acting on plasminogen

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14
Q

plaminogen-plasmin binding proteins

A

convert plasminogen to plasmin and break up clots

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15
Q

post strep sequelae

A

glomerulonephritis, rheumatic fever

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16
Q

glomerulonephritis

A

organism-Ab complex deposit in glomerulus binds Cā€™ causing inflammatory response

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17
Q

rheumatic fever

A

Abs x-react w/ heart tissue, autoimmune

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18
Q

Strep pyogenes group and biochems:
hemolysis
PYR
bacitracin
SXT
CAMP
hippurate hydrolysis

A

GAS
beta hemo
PYR pos
A disk sus
SXT R
CAMP/hipp neg

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19
Q

PYR

A

L-pyrrolidonyl beta napthylamide hydrolysis

20
Q

strep pneumo biochems:
heme
catalase
optochin
virulence

A

alpha hemo
catalase neg
optochin sus
capsule

21
Q

Enterococcus biochems:
heme
catalase
bile esculin
PYR
NaCl

A

alpha hemo
catalase neg
bile esculin pos
PYR pos
6.5 NaCl pos

22
Q

Group D strep biochems
Heme
catalase
bile esculin
PYR
NaCl

A

alpha/gamma hemo
catalase neg
bile esculin pos
PYR/6.5 NaCl neg

23
Q

Bacitracin/A disk

A

susceptible is presumptive pyogenes/group A

24
Q

Strep ID flow Beta hemo

A
  1. PYR or bacitracin
  2. CAMP or hippurate hydrolysis
  3. bile esculin
  4. PYR or 6.5% NaCl
25
Q

Strep ID flow alpha hemo

A
  1. Optochin P disk
  2. bile esculin
  3. PYR bacitracin 6.5% NaCl
26
Q

Strep ID flow gamma hemo

A
  1. CAMP or hippurate
  2. bile esculin
  3. PYR or bacitracin or 6.5% NaCl
27
Q

SXT trimethoprim-sulfmethoxazole disk resistance

A

GAS/GBS resistant

28
Q

PYR test

A

detects pyrrolidonyl arylamidase

29
Q

PYR reagent

A

N,N dimethylaminocinnamaldehyde

30
Q

GAS pyogenes treatment

A

penicillin or erythromycin

30
Q

GAS pyogenes treatment

A

penicillin or erythromycin

31
Q

GBS media

A

Todd-Hewitt, Trans-Vag broth, LIM, carrot

32
Q

GBS media

A

Todd-Hewitt, Trans-Vag broth, LIM, carrot

33
Q

CAMP test

A

streak perpendicular to S. aureus, beta lysin + CAMP factor = arrow head hemo, presumptive agalactiae

34
Q

Hippurate hydrolysis test

A

GBS, Sodium benzoate precipitate with ferric Cl, glycine purple with ninhydrin

35
Q

GBS agalactiae treatment

A

penicillin G, clindamycin, vancomycin

36
Q

Group C, F G strep ID

A

Beta hemo, use serogrouping

37
Q

pneumo morphology

A

diplococci, alpha hemo, autolyse

38
Q

pneumo virulence factors (3)

A

capsule
IgA protease
pneumolysin

39
Q

pneumolysin

A

similar to streptolysin O

40
Q

bile solubility test

A

Na deoxycholate accelerates autolysis, clear tube = pos

41
Q

enterococcus morphology

A

small, smooth, grey-blue colonies, alpha or gamma hemo

42
Q

bile esculin test

A

ability to grow in 40% bile and hydrolyze esculin, black precipitate pos

43
Q

6.5% salt tolerance test

A

pH indicator yellow pos enterococcus, negative purple/clear

44
Q

Viridans strep ID

A

catalase neg, optochin resistant, bile esculin neg, not bile soluble, no capsule

45
Q

satelliting strep abiotrophia and granulicatella are ___ deficient

A

B6 deficient, can grow with S. aureus

Micro (11 decks)

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