Gram Positive Bacilli Flashcards

1
Q

Bacillus Anthracis Virulence Factors

A

Edema factor
protective antigen
lethal factor
spores

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Bacillus anthracis biochemistry
heme
motility
catalase
gram morphology

A

gamma hem
nonmotile
catalase POS
GPC string of pearls

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Bacillus anthracis infection is acquired from

A

livestock by contamination, ingestion or inhalation

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Bacillus cereus infections are

A

Food poisoning, eye infections

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Bacillus subtiles is a common ____________ in the lab

A

Common contaminant

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Cutaneous anthrax

A

“maliguant pustule”, eschar blackening

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Pulmonary anthrax

A

“Woolsorters disease”, inhalation of spores

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Gastro anthrax

A

Ingestion of contaminated raw meat

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Anthrax treatment/prevention

A

Penicillin, ciprofloxacin, vaccine not very effective

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Anthrax BAP morphology

A

Medusa head
ground glass
tenacious
gamma

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Bacillus cereus infection

A

food poisoning
line contamination
eye infections

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Emetic form

A

Heat stable
fried rice
1-5hr incubation

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Diarrheal form

A

Heat labile
meat and veg
8-16hr incubation

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

B cereus biochems:
heme
motility
gelatin hydrolysis
penicillin

A

Beta
mobile
gelatin hydrolysis pos
penicillin resistant

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Corneybacterium sp. biochem
Heme
motility
catalase
gram stain

A

gamma
non-motile
catalase POS
palisading “club” w/ metachromatic granules

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Corynebacterium diphtheria toxin

A

Lysis during phagocytosis causes activation

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

Toxin A fragment

A

Inhibits protein synthesis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

Toxin B fragment

A

Mediates entry of fragment A

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

Corynebacterium diphtheria pathogenesis

A

Thick pseudomembane, bull neck

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

C. diphtheriae identification:
agar type
heme
catalase
esculin hydrolysis
elek test

A

Tellurite agar
alpha
catalase POS
esculin hydrolysis neg
elek POS

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

Diphtheria treatment

A

Antitoxin from horses, penicillin, erythromycin

22
Q

Listeria monocytogenes biochems:
hemolysis
catalase
motility
bile esculin
hipp hydrolysis
CAMP

A

Beta
Catalase POS
motile @ 25C
bile esculin pos
hippurate hydrolysis POS
CAMP block hem.

23
Q

Listeria monocytogenes treatment

A

Penicillin, ampicillin, gentamicin

24
Q

Erysipelothrix rhusopathiae infections

A

Erysipeloid
Endocarditis w/ valve replacements
Acquired from livestock

25
Q

Erysipelothrix rhusiopathiae biochem
heme
growth req
catalase
motility
H2S

A

alpha
CO2 required
Catalase neg
non motile
produces H2S on TSI

26
Q

Lactobacillus species biochem
heme
catalase
gram stain

A

gamma/alpha
Catalase neg
gram POS bacilli

27
Q

Arcanobacterium haemolyticum infection

A

Pharyngitis in adults 10-20 years

28
Q

Arcanobacterium haemolyticum biochem:
heme
catalase
CAMP
Gram morphology

A

beta
Catalase neg
reverse CAMP POS
gram POS pleomorphic

29
Q

Gamma gram POS bacilli (4)

A

Bacillus anthracis
Corynebacterium
Lactobacillus
Erysipelothrix

30
Q

Beta gram POS bacilli

A

Arcanobacterium haemolyticum
Bacillus (not anthracis)
Listeria

31
Q

Alpha gram POS rods

A

Lactobacillus, erysipelothrix

32
Q

If catalase POS, then:

A

Motility testing

33
Q

If catalase POS, motility POS, then:

A

BEA

34
Q

If catalase POS, motility POS, BEA pos, then:

A

Listeria monocytogenes

35
Q

If catalase POS, motility POS, BEA neg, then:

A

Bacillus species

36
Q

If catalase POS, motility neg, then:

A

Spores testing

37
Q

If catalase POS, motility neg, spore POS, then:

A

Rule out Bacillus anthracis

38
Q

If catalase POS, motility neg, spore neg, then:

A

Corynebacterium species

39
Q

If catalase neg, then:

A

H2S production

40
Q

If catalase neg, H2S POS, then:

A

Erysipelothrix rhusiopathiae

41
Q

If catalase neg, H2S neg, then:

A

Hemolysis

42
Q

If catalase neg, H2S neg, beta, then:

A

Arcanobacterium haemolyticum

43
Q

If catalase neg, H2S neg, gamma, then:

A

Lactobacillus species

44
Q

Actinomycetes nocardia species morphology

A

Aerobe, gram POS/variable, weakly acid fast, resemble fungi, branching

45
Q

Nocardia infections

A

Nocardiosis, mycetomas, pulmonary infections

46
Q

Modified acid-fast stain

A

Red/purple=acid fast, blue = negative

47
Q

Aerobic Actinomycetes culture reqs

A

Inhibited by chloramphenicol, 4 weeks @ 30C, wide variety of media

48
Q

Nocardia morphology

A

Chalky, matte, dry, may be pigmented, wrinkled

49
Q

Nocardia biochem:
catalase
motility
lysozyme

A

Catalase POS
motility =
lysozyme resistant

50
Q

Nocardia brasiliensis decomposition/hydrolysis tests (4)

A

Starch, casein, tyrosine POS
xanthine neg