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Gram Positive Bacilli Flashcards

1
Q

Bacillus Anthracis Virulence Factors

A

Edema factor
protective antigen
lethal factor
spores

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2
Q

Bacillus anthracis biochemistry
heme
motility
catalase
gram morphology

A

gamma hem
nonmotile
catalase POS
GPC string of pearls

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3
Q

Bacillus anthracis infection is acquired from

A

livestock by contamination, ingestion or inhalation

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4
Q

Bacillus cereus infections are

A

Food poisoning, eye infections

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5
Q

Bacillus subtiles is a common ____________ in the lab

A

Common contaminant

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6
Q

Cutaneous anthrax

A

“maliguant pustule”, eschar blackening

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7
Q

Pulmonary anthrax

A

“Woolsorters disease”, inhalation of spores

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8
Q

Gastro anthrax

A

Ingestion of contaminated raw meat

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9
Q

Anthrax treatment/prevention

A

Penicillin, ciprofloxacin, vaccine not very effective

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10
Q

Anthrax BAP morphology

A

Medusa head
ground glass
tenacious
gamma

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11
Q

Bacillus cereus infection

A

food poisoning
line contamination
eye infections

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12
Q

Emetic form

A

Heat stable
fried rice
1-5hr incubation

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13
Q

Diarrheal form

A

Heat labile
meat and veg
8-16hr incubation

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14
Q

B cereus biochems:
heme
motility
gelatin hydrolysis
penicillin

A

Beta
mobile
gelatin hydrolysis pos
penicillin resistant

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15
Q

Corneybacterium sp. biochem
Heme
motility
catalase
gram stain

A

gamma
non-motile
catalase POS
palisading “club” w/ metachromatic granules

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16
Q

Corynebacterium diphtheria toxin

A

Lysis during phagocytosis causes activation

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17
Q

Toxin A fragment

A

Inhibits protein synthesis

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18
Q

Toxin B fragment

A

Mediates entry of fragment A

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19
Q

Corynebacterium diphtheria pathogenesis

A

Thick pseudomembane, bull neck

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20
Q

C. diphtheriae identification:
agar type
heme
catalase
esculin hydrolysis
elek test

A

Tellurite agar
alpha
catalase POS
esculin hydrolysis neg
elek POS

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21
Q

Diphtheria treatment

A

Antitoxin from horses, penicillin, erythromycin

22
Q

Listeria monocytogenes biochems:
hemolysis
catalase
motility
bile esculin
hipp hydrolysis
CAMP

A

Beta
Catalase POS
motile @ 25C
bile esculin pos
hippurate hydrolysis POS
CAMP block hem.

23
Q

Listeria monocytogenes treatment

A

Penicillin, ampicillin, gentamicin

24
Q

Erysipelothrix rhusopathiae infections

A

Erysipeloid
Endocarditis w/ valve replacements
Acquired from livestock

25
Erysipelothrix rhusiopathiae biochem heme growth req catalase motility H2S
alpha CO2 required Catalase neg non motile produces H2S on TSI
26
Lactobacillus species biochem heme catalase gram stain
gamma/alpha Catalase neg gram POS bacilli
27
Arcanobacterium haemolyticum infection
Pharyngitis in adults 10-20 years
28
Arcanobacterium haemolyticum biochem: heme catalase CAMP Gram morphology
beta Catalase neg reverse CAMP POS gram POS pleomorphic
29
Gamma gram POS bacilli (4)
Bacillus anthracis Corynebacterium Lactobacillus Erysipelothrix
30
Beta gram POS bacilli
Arcanobacterium haemolyticum Bacillus (not anthracis) Listeria
31
Alpha gram POS rods
Lactobacillus, erysipelothrix
32
If catalase POS, then:
Motility testing
33
If catalase POS, motility POS, then:
BEA
34
If catalase POS, motility POS, BEA pos, then:
Listeria monocytogenes
35
If catalase POS, motility POS, BEA neg, then:
Bacillus species
36
If catalase POS, motility neg, then:
Spores testing
37
If catalase POS, motility neg, spore POS, then:
Rule out Bacillus anthracis
38
If catalase POS, motility neg, spore neg, then:
Corynebacterium species
39
If catalase neg, then:
H2S production
40
If catalase neg, H2S POS, then:
Erysipelothrix rhusiopathiae
41
If catalase neg, H2S neg, then:
Hemolysis
42
If catalase neg, H2S neg, beta, then:
Arcanobacterium haemolyticum
43
If catalase neg, H2S neg, gamma, then:
Lactobacillus species
44
Actinomycetes nocardia species morphology
Aerobe, gram POS/variable, weakly acid fast, resemble fungi, branching
45
Nocardia infections
Nocardiosis, mycetomas, pulmonary infections
46
Modified acid-fast stain
Red/purple=acid fast, blue = negative
47
Aerobic Actinomycetes culture reqs
Inhibited by chloramphenicol, 4 weeks @ 30C, wide variety of media
48
Nocardia morphology
Chalky, matte, dry, may be pigmented, wrinkled
49
Nocardia biochem: catalase motility lysozyme
Catalase POS motility = lysozyme resistant
50
Nocardia brasiliensis decomposition/hydrolysis tests (4)
Starch, casein, tyrosine POS xanthine neg

Micro (11 decks)

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