Non-fermenting GNR Flashcards
Non-fermenters are non reactive on ___ and ___ slants
TSI and KIA
______ use CHO for energy in aerobic and anaerobic conditions
Fermenters
_____ cannot break down CHOs in anaerobic conditions
Non-fermentors
_____ metabolize CHOs in air
Oxidizers
_____ cannot use CHOs as an energy source at all
Non-oxidizer
Most non-fermenters are oxidase ___ and lactose ___
Pos, neg
Oxidative-Fermentative (OF) test determines
if MO is a fermenter, oxidizer, or non oxidizer
Asacchrolytic
unable to break down CHOs for energy
Cause of most GNNF infections
Pseudomonas aeruginosa
Pseudomonas motility
motile with polar flagella
Pseudomonas oxidase and catalase
positive
Pseudomonas fermentation/oxidation
non-fermenter (TSI k/k)
oxidizer
Pseudomonas has a ___ ______ capsule
Mucoid exopolysaccharide
Textbook P. aeruginosa description
pitted
fringy edge
beaten copper
grape-like odor
pyocyanin color
blue green
pyorubin color
red
pyomelanin color
brown black
pyoverdin color
yellow, fluoresces
Virulence factors of P. aeruginosa lipopolysaccharide
endotoxin
pyrogen
Virulence factors of P. aeruginosa capsule
anti phagocytic
inhibits complement
Virulence factors of P. aeruginosa proteases
damage host proteins
Virulence factors of P. aeruginosa exotoxin A
blocks protein synthesis
Virulence factors of P. aeruginosa pyocyanin
impairs ciliary activity
Virulence factors of P. aeruginosa fimbrae
adherence factor
P. aeruginosa is able to grow at temps of ___
42C
P. aeruginosa is nitrate ___
pos
Acinetobacter catalase
pos
Acinetobacter oxidase
pos
Acinetobacter motility
non-motile
Acinetobacter is able to grow at temps of ___
20-30C
Acinetobacter nitrate
neg
A. baumannii OF test
Saccharolytic, oxidizer
A. lowffii OF test
asaccharolytic, cannot use CHO
Stenotrophomonas maltophilia habitat
aquatic environments
Stenotrophomonas maltophilia habitat
aquatic environments
Stenotrophomonas maltophilia oxidase
neg
Stenotrophomonas maltophilia usually susceptible to
trimethoprim-sulfamethoxazole (SXT)
Stenotrophomonas maltophilia LDC
pos
Stenotrophomonas maltophilia DNAse
pos
Stenotrophomonas maltophilia ONPG
pos
Burkholderia cepacia oxidase
pos
cepacia syndrome
severe respiratory disease in CF patients
Burkholderia cepacia selective agars
BCSA
OFPBL
yellow on both
Burkholderia pseudomallei causes the disease
mellioidosis
bioterrorist agent
Burkholderia pseudomallei