Storage and Retrieval of Genetic Information Flashcards
What are the differences between RNA and DNA
DNA = H on C2 = stable RNA = OH on C2 = Can attach to phosphate causing denaturation of 2 RNA molecules. Not good long term store of information
What is upstream and downstream
3 prime = downstream
5 prime = upstream
What are genes
Structural information coding for a protein
What are Gene regulatory elements
Promotor = close to gene
Enhancer = further way from gene
Bind to specific transcriptional activators
What are non-coding DNA
Exon = coding
Intron = non-coding
Introns are removed from primary DNA in RNA
What are the building blocks for DNA synthesis
Deoxynucleoside trisphosphate
What direction does DNA replication occur in
5 prime to 3 prime
What do primers have that allow DNA replication
Free 3 primes to receive dNTPs
Where does the energy come from
Hydrolysis of trisphosphate to 1 when dNTP 5 prime added to 3 prime
What is the difference between leading and lagging strand
Leading strand = continuous synthesis
Lagging strand = discontinuous synthesis. Okazaki fragments made then back-stitched together
What does DNA polymerase do
Proof reads code
What is the building block for transcription
Nucleoside trisphosphate
What is the splicesome
Enzymes responsible for splicing on RNA polymerase then jump on RNA transcript to remove introns
How many reading frames are there for the genetic code
3
