Sterilization And Disinfection Flashcards
Used to decontaminate certain biological waste and sterilized media instruments and lab ware
Autoclave
What are the different types of autoclave
- Pressure cooker type
- Horizontal autoclave
- Vertical autoclave
- Common laboratory autoclave
- Large automatic hospital autoclave
The first autoclave like pressurized vessels similar to what is known as pressure cooker
Denis papin
When did denis papin was built
1679
Work with louis pasteur to create first autoclave for medical and scientific purposes
Charles chamberland
When did pasteur and chamberland build autoclave for Medical and scientific purposes
1879
Refer to as a steam sterilizer
Sterilization
What are the four aspect vital to effective sterilization
Time
Temperature
Pressure
Steam
What is the standard time for autoclave
15 minutes
What is the standard autoclave temperature
121°C (250°F)
Does the boiling point of water kill endospores
No
Is used as an indicator to confirm that successful sterilization cycle was completed
Geobacillus stearothermophilus
What is the minimum pressure needed in autoclave
15 psi
It provides insulation to pathogens from the sterilizing environment and are removed during pressure ramping so that pathogens do not escape proper sterilization
Air pockets
Transfer of heat is mostly efficiently done using
Steam
Refers to a liquid culture medium
Broth
It indicates bacterial growth in a liquid state
Turbidity
Broth are usually kept in a ____ and _____
Test tube and flask
It is known as dehydrated culture media that used to grow and isolate microorganism
TSB (Tryptic Soy Broth)
TSB served as the source of _____ and _____for the microorganism
Nitrogen and amino acid
Refers to a solid culture medium used to grow microbes on a plate
Agar
Agar is a ____ like substance derived from _____ that is used in food, medicine, and microbiology
Jelly, red algae
What are the various types of agar
Nutrient agar
Blood agar
MacConkey agar
EMB agar
XLD agar
A common medium for cultivating an isolating a wide range of microorganisms, it also supports the growth of most non fastidious bacteria
Nutrient agar
Technique used to culture microorganisms on agar plate
Plating
Technique used to estimate the number of microorganism in a sample with an unknown concentration
Serial dilution
It involves diluting a sample multiple times to reduce the concentration to a countable level
Serial dilution
What are the common plating techniques
Streak plate method
Spread plate method
Pour plate method
A loop full of specimen is straight across an agar plate in an zinzag pattern
Streak plate method
A liquid or suspension sample is spread across an agar plate
Spread plate method
A liquid sample is mixed with molten agar and poured into a petri dish
Pour plate method
A laboratory technique used to isolate pure cultures of bacteria from mixed population
Streak plate method
Widely used culture techniques in microbiology laboratories due to its ease and simplicity
Spread plate method
Sample of spread plate method must be in _____ or _____
Liquid or suspension
A tool used to smoothly spread cells and bacteria on a culture plate
Plate spreader or cell spreader
Suitable for facultative microerophilic and anaerobic microorganism
Pour plate method
A laboratory instrument used to measure and transfer very small amounts of liquid
Micropipette
A small disposable attachment used with a micropipette to accurately measure and transfer very small volume of liquid
Micropipette tips
