Sterilization Flashcards
Sterilization
Killing or removing all forms of microbial life in a material or an object
What is the most common way of sterilization ?
Heat
Spore structure
Hibernation , thick outer polypeptide that is hardly resistant
Bacteria that has spores
Bacillus anthrax,
Physical methods of sterilization
Freezing,
Ionizing
particulate electron beam
Filtration
Small pore(0.2 um)
HEPA filter
High efficiency particulate, used electromagnetic methods.
Dry heat
Incineration, flaming, desiccation, hot air sterilization
Moist heat
Coagulates protein and break hydrogen bonds
Example of moist heat
Boiling and stream
What is tyndallisation?
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Autoclaving
Steaming under pressure forces heat into cells.
What is the temp of autoclaving?
121
How an autoclave works ?
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