Sterilization Flashcards
Sterilization
Killing or removing all forms of microbial life in a material or an object
What is the most common way of sterilization ?
Heat
Spore structure
Hibernation , thick outer polypeptide that is hardly resistant
Bacteria that has spores
Bacillus anthrax,
Physical methods of sterilization
Freezing,
Ionizing
particulate electron beam
Filtration
Small pore(0.2 um)
HEPA filter
High efficiency particulate, used electromagnetic methods.
Dry heat
Incineration, flaming, desiccation, hot air sterilization
Moist heat
Coagulates protein and break hydrogen bonds
Example of moist heat
Boiling and stream
What is tyndallisation?
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Autoclaving
Steaming under pressure forces heat into cells.
What is the temp of autoclaving?
121
How an autoclave works ?
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Thermization
The application to milk of a heat treatment of a lower intensity than pasteurization that aims at reducing the number of microorgansations
Commercial sterilization
The application of heat at high temperatures for a time sufficient to render milk or other products commercially stable.
Pasteuration
The application of heat to milk and liquid milk products aimed at reducing the number of any pathogenic microorganisms to a level at which they do not contribute
UHF pasteurization
Ihdiha
Chemical halogen and halogen contains compounds.
Chlorine, iodine, iodophores , bromine, fluorine
Miscallenous
Gaseous, heavy metals, dyes, acids and alkalies.
Chemical used in sterilization
Phenols, alcohols , peroxide, aldehydes
Combination of alcohol and iodine
Good killing effect
Chlorhexidine glauconite
Does not kill tb or spores
Osmotic pressures.
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