steri and disin Flashcards
It is a complete destruction or elimination of all forms of microbial life including bacteria’s viruses fungi any spores
Sterilization
Reduction of the number of pathogenic microorganisms on sir face or in animate object to a level considered safe or public health
Disinfection
Six physical methods to and he beat microbial growth
Heat pressure desiccation radiation sonic disruption filtration
It is most efficient practical and inexpensive method for in animate object and materials that can with stand high temperature
Heat
It kills by coagulating proteins
Heat
Factors in heat
Heat and time
The higher the temperature the shorter the time required to kill the organisms
True
It is the lowest temperature that will kill all organisms in a standardize pure culture within a specified period.
Thermal death point
The link of the time necessary to sterilize if your culture at a specified temperature
Thermal death time
Kills by oxidation
Dry heat
coagulation accompanied by hydrolysis
Moist heat
Example of dry heat
Hot air oven and incineration
temp in Hot air oven within for 2 hours
160°C to 165°C
Temperature in Hot air all then for one hour
170° to 180°C
It is a type of dry heat for metals glassware some powders audience and waxes
Hot air Ovan
Is it a type of dry heat for contaminated disposable materials
Incineration
Four wire loops, wire needles, four steps, mouth of tube media and dream of petri dishes
Flaming
Example of moist heat
Autoclave boiling fractional sterilization inspissation pasteurization
Celsius and minutes in boiling
100°C for 15 to 30 minutes
Type of moist heat that kills vegetative forms but not spores and viruses
Boiling
It uses steam under pressure
Auto clave
Principle of autoclave
121°C at 15 psi for 15 to 30 minutes
It steams for 30 minutes for three consecutive days
Fractional sterilization
inspissation princple
75 to 80°C for two hours for three successive days
It does not kill microorganisms but it slows down their metabolism
Cold
It is slows down the growth
Refrigeration
It is not used because ice crystals are formed
Snow freezing
It uses nitrogen and for preservation
Rapid freezing
It places bacteria under suspended animation
Rapid freezing
Foods may be preserved by drying
Desiccation
Example of desiccation
n gonorrhea and MTB
It is for prevention of food spoilage, sterilization of heat sensitive equipment and preparation of vaccine
radiation
Four types of radiation
Sunlight, UVR, x-rays, gamma rays
It is a type of registration that kills only those exposed to direct sunlight
Sunlight
It is a type of registration which uses UV lamp or germicidal lamp
UVR
It may be placed in the rooms, cabinets containing instruments, close equipment, liquid and other in animate object
You VR
It is a type of registration which reduces number of microorganisms in the air
You VR
Disadvantages of UVR
DNA replication is inhibited, form thymine dimers, can damage the cornea and skin and do not penetrate load blast and metals