cul and cul med Flashcards by Glory Resusta

Material containing the necessary nutritional and environmental requirements for bacterial growth

Culture medium

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It is a liquid, semi solid or solid preparation utilized to observe growth pattern of microorganisms as well as for transport and the storage

Culture medium

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Three types of culture

Pure culture, mix culture, stock culture

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If the culture is for academic and industrial purposes

Stock culture

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If the culture is more than 2 genus and species

Mix culture

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If the culture is one genus

Pure culture

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agar percent in semi solid

0.5% to 1%

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Agar percent in solid

2 to 3%

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It allows growth of a robes, and a robes and facultative and a robes

Liquid

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What physical state if the auger is clot like consistency

Semi solid

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Medium of neisseria

Thayer Martin medium
Martin lewis medium
new york city medium

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medium of staphylococcus

MANNITOL SALT AGAR
PHENYL ETHYL ALCOHOL AGAR
VOGEL AND JOHNSON AGAR
CHAPMANS STONE AGAR

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group A strepto medium

CRYSTAL VIOLET BLOOD AGAR
POLYMYXIN B, NEOMYCIN FUSIDIC ACID MEDIUM

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group b strepto

LIM, CARROT BROTH
TODD AND HEWITT BROTH W ANTIBIOTICS
GRANADA AGAR

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vibrio

THIOSULFATE CITRATE BILE SALT SUCROSE
MONSUR TELLURITE

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brucella

CASTANEDA MEDIUM
WISCONSIN MEDIUM

BORDETELLA

BORDET GENGOU
JONES KENDRICK CHARCOAL
REGAN LOWE
CAMPHYLOBACTER SKIRROWS

yersinia medium

scheimanns selective medium

clostridium medium

COOKED MEAT MEDIUM
THIOGLYCOLATE BROTH
SCHAEDLERS MEDIUM

corynebacterium medium

CYSTINE TELLURITE
TINSDALE MEDIUM
LOEFFLERS MEDIUM

It is a type of medium to isolate bacteria with specific growth requirement

Special media

Example of culture medium

Middlebrook 7H 10, 7H 11- mycobacterium and wilkin chalgren agar for anaerobic bacteria

Type of medium that distinguishes group of organisms based on cultural characteristics

Differential culture medium

Example of differential culture medium

HEKTOEN ENTERIC AGAR
MACCONKEY
BAP

It allows visualization of metabolic differences between group of species of bacteria, this is a type of medium

Differential culture medium

It is a type of differential contour medium that contains bio salts and dies to selectively slow the growth of most non-pathogenic gram-negative bacilli found in the gastrointestinal tract

HEA

It is a type of differential culture medium that has lactose Permenter’s and unlock those fermenters

macconkey

Color of lactose fermenters in macconkey

pink

Color of non-lactose fermenters

colorless

Preferred blood for the preparation of BAP

SHEEP HORSE RABBIT

Two types of enriched culture medium

Blood agar and chocolate agar

enriched medium for most fastidious bacteria

Blood agar

enriched culture medium for haemophilus and neisseria

Chocolate agar

Human blood is not preferred because it contains non-specific inhibitors like

Citrate and dextrose

Culture media according to composition

Synthetic or defined medium, nonsynthetic or complex medium, tissue culture medium

Very useful for the isolation of bacteria

Nonsynthetic or complex medium

To isolate obligate intracellular bacteria

Tissue culture medium

It is used for research purposes

Synthetic or defined medium

It’s supplies 10 and five factors

Chocolate agar