Steps of Glycolysis Flashcards

1
Q

—– transports glucose into cell
1) —– to —– via —–, requiring —–
2) —– to —– via —–
3) —– to —– via —–, requiring —–
4) —– to —– and —– via —–
5) —– to —– via —–
6) —– to —– via —–, yielding —–
7) —– to —– via —–, yielding —–
8) —– to —– via —–
9) —– to —– via —–
10) —– to —– via —–, yielding —–

A

GLUT1 transports glucose into cell
1) glucose to G6P via hexokinase, requiring 1 ATP
2) G6P to F6P via phosphoglucoisomerase
3) F6P to F16P via phosphofructokinase, requiring 1 ATP
4) F16P to G3P and DHAP via aldolase
5) DHAP to G3P via triose phosphate isomerase
6) G3P to 1,3BG via glyceraldehyde-3-phosphate dehydrogenase, yielding 2 NADH total
7) 1,3BG to 3PG via phosphoglycerate kinase, yielding 2 ATP total
8) 3PG to 2PG via phosphoglycerate mutase
9) 2PG to PEP via enolase
10) PEP to pyruvate via pyruvate kinase, yielding 2 ATP total

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2
Q

rxn 1 type

A

phosphorylation

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3
Q

rxn 2 type

A

isomerization

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4
Q

rxn 3 type

A

phosphorylation

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5
Q

rxn 4 type

A

cleavage
(reverse aldol)

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6
Q

rxn 5 type

A

isomerization

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7
Q

rxn 6 type

A

oxidation via phosphorylation

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8
Q

rxn 7 type

A

oxidation via dephosphorylation

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9
Q

rxn 8 type

A

isomerization

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10
Q

rxn 9 type

A

dehydration

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11
Q

rxn 10 type

A

dephosphorylation

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12
Q

NADH abbreviation

A

nicotinamide adenine dinucleotide

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13
Q
A

NAD+

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14
Q

FADH2 abbreviation

A

flavin adenine dinucleotide

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15
Q
A

FADH2

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16
Q

glycolysis net gain

A

2 ATP
2 NADH

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17
Q

glycolysis net gain

A

2 ATP
2 NADH

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18
Q

investment phase

A

rxn 1-5

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19
Q

payout phase

A

rxn 6-10

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20
Q

maintains gradient for GLUT1

A

negative charge on G6P - cannot leave cell

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21
Q

why F16P?

A

it is unstable and almost symmetrical

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22
Q

commitment step

A

rxn 3
F16P must proceed

23
Q

aldolase Km

A

52
high affinity

24
Q

rxn that doubles the investment

A

rxn 4

25
Q

as G3P moves forward into rxn 6…

A

equilibrium shifts toward G3P, away from DHAP, until there is no more DHAP

26
Q

captured by NAD+

A

hydride

27
Q

first substrate-level phosphorylation

A

rxn 7

28
Q

unstable and highly reactive due to enol group

A

PEP

29
Q

second substrate level phosphorylation

A

rxn 10

30
Q
A
31
Q
A
32
Q
A
33
Q
A

rxn 1

34
Q
A

rxn 2

35
Q
A

rxn 3

36
Q
A

rxn 4

37
Q
A

rxn 5

38
Q
A

rxn 6

39
Q
A

rxn 7

40
Q
A

rxn 8

41
Q
A

rxn 9

42
Q
A

rxn 10

43
Q
A

glucose

44
Q
A

G6P

45
Q
A

F6P

46
Q
A

F16P

47
Q
A

F16P

48
Q
A

G3P

49
Q
A

DHAP

50
Q
A

1,3BG

51
Q
A

3PG

52
Q
A

2PG

53
Q
A

PEP

54
Q
A

pyruvate