Past Exam Questions Flashcards
endomembrane system
- golgi
- smooth ER
- rough ER
- nuclear envelope
protein folding inside…
rough ER
role of Golgi
PTMs, packing and delivering
true of endocytosis?
utilizes receptor proteins
gel runs –’ to –’
3’ to 5’
3 components of nucleotides
sugar
base
phosphage
4 factors affecting 3D structure of DNA
- base pairing with H bonds
- base stacking with pi-pi interactions
- syn/anti conformers
- ribose conformers
5 steps for DNA –> protein sequence
- 3’ to 5’ complementary DNA strand
- 3’ to 5’ complementary mRNA strand (Ts to Us)
- 5’ to 3’ complementary mRNA strand (just flip)
- codons to amino acids
- draw amino acids
B-sheet stabilized by…
H-bonds between 2 parts of polypeptide so that those segments are held together side by side
NOT characteristic of globular protein
highly repetitive in 1° structure
Peptide sequence LEAST likely to be found in interior core of globular protein
DKYPGE
PTM example
phosphotyrosine
stabilizes alpha helices
H bonds between peptide groups four positions along the polypeptide
heteropolysaccharides
peptidoglycan
hyaluronic acid
inhibition
parallel lines on LB plot
uncompetitive
Vmax decreases
Km decreases
inhibition
flattened curve, same Vmax
competitive
Km increases
inhibition
same X int, different slopes
LB plot
noncompetitive
Vmax decreases
Km not changed
hexokinase inhibited by high…
G6P
substrate for heme in TCA
succinyl CoA
No FeS clusters
complex IV
NOT involved in rxns for FA synthesis
oxidation
NOT involved in rxns for FA breakdown
phosphorylation
rounds of B-oxidation needed for 18-C chain FA
8
(18/2)-1
moves FAs into mitochondria for B-oxidation
acyl-CoA shuttle
