Staphlococci!! MICROM 442 Deck 11

Question 1

staphlococcus

Answer 1

purple grapes baby

Question 2

gram positive cocci arranged as

Answer 2

singles, pairs, tetrads, clusters and divide in multiple perpendicular planes

Question 3

what type of anaerobe?

Answer 3

facultative

Question 4

all staphlococci are positive for??

Answer 4

catalase

Question 5

non-motile and also

Answer 5

non-spore forming

Question 6

species differentation

Answer 6

1. Phenotype on blood agar
2. Coagulase: bacterial protein that causes clotting of blood plasma
3. mannitol fermentation
4. rapid diagnostic methods

Question 7

phenotype on blood agar

Answer 7

• pigment production: S. aureus (gold) and S. epidermidis (white)
• hemolysis: S. aureus (β-hemolytic) and S. epidermidis (γ-hemolytic)

Question 8

coagulase

Answer 8

• surface bound: cleaves fibrinogen → fibrin (precipitates)
• extracellular: binds prothrombin, cleaves fibrinogen → fibrin

Question 9

which one ferments mannitol?

Answer 9

s. aureus

Question 10

majority of CoNS can NOT ferment

Answer 10

mannitol

Question 11

what media is used to test for mannitol fermentation

Answer 11

mannitol salt agar which is both differential and selective

Question 12

what color does MSA turn of it can ferment mannitol?

Answer 12

yellow

Question 13

examples of rapid diagnostic methods?

Answer 13

-PCR
_MALDI-TOF-MS

Question 14

S. aureus characteristics

Answer 14

-gold color -> membrane bound cartenoid called staphlyoxanthin which is a virulence factor
-coagulase+
-ferments mannitol
-beta-hemolytic

Question 15

Human Colonization by S. aureus

Answer 15

Colonizes the skin and mucosa of asymptomatic individuals
- common sites: anterior nares (most), hand, perineum, and pharynx
* 3 nasal carriage patterns in healthy individuals
- non-carriage, intermittent, and persistent

Question 16

S.A. MSCRAMM

Answer 16

Protein A -> von Willebrand factor (vWF) - platelets

Question 17

S.A. SERAM

Answer 17

Extracellular Adherence Protein (Eap) -> fibrinogen and fibronectin

Question 18

S.A Invasins: extracellular proteins lyse cells and promote spread -> pore-forming

Answer 18

-Hemolysins -> lyse erythrocytes and other mammalian cells
-Panton-Valentine Leukocidin -> lyse neutrophils and macrophages