Staph Cont.

Question 1

CoNS

_______: predominantly hospital acquired

1. predisposing factors: instrumentation procedures (3)
2. common cause of _____ & _____
3. treated w/ _____ & _____
4. normal flora of skin & mucus membrane but can cause hospital acquired due to production of _____ or ______
   - which contains ______

Answer 1

Staphylococcus epidermidis

1. catheterization, medical implantation & immunosuppressive therapy
2. healthcare-acquired UTIs & heart valve endocarditis
3. vancomycin & rifampin
4. biofilm or slime
   - poly-gamma-DL-glutamic acid

Question 2

CoNS

_______: predominantly hospital acquired

1. associated with _____ amount young women

______: causes both community-associated & hospital acquired infections
1. more aggressive than other CoNS since this contains ____
2. slide method = _____; tube method = _____
3. disease caused (4)

Answer 2

Staphylococcus saprophyticus
1. UTIs

Staphylococcus lugdunensis
1. mecA gene
2. positive ; negative
3. TSS, Osteomyelitis, Septic arthritis, Endocarditis

Question 3

Tests S.epidermidis vs S.saprophyticus

1. colony
2. catalase
3. Coagulase
4. mannitol
5. hemolysis
6. gelatinase
7. DNAse test
8. novobiocin
   - S.epidermidis
   - S.saprophyticus
9. phosphatase
   - S.epidermidis
   - S.saprophyticus
10. virulence factor
    - S.epidermidis
    - S.saprophyticus

Answer 3

1. white colonies
2. positive
3. negative
4. negative
5. gamma
6. positive
7. negative
8. novobiocin
   - susceptible
   - resistant
9. phosphatase
   - positive
   - negative
10. virulence factor
    - slime/poly-gamma-DL-glutamic acid
    - uncertain

Question 4

Other Staphylococci spp.

1. dog infection
   - slide coagulase
   - PYR
   - VP
2. nosocomial infection and vancomycin resistant
   - coagulase
   - hemolytic

Answer 4

1. Staphylococcus intermedius
   - POSITIVE
   - POSITIVE
   - NEGATIVE
2. Staphylococcus haemolyticus
   - NEGATIVE
   - BETA HEMOLYTC

Question 5

Staph vs Micrococci

1. modified oxidase (presumptive test)
   - Staph
   - Micrococci
2. aerobic acid production from ____
   - Staph
   - Micrococci
3. anaerobic acid production from _____ (presence of erythromycin)
   - Staph
   - Micrococci

Answer 5

1.
- NEGATIVE
- POSITIVE
2. glucose
- POSITIVE
- NEGATIVE
3. glycerol
- POSITIVE
- NEGATIVE

Question 6

Staph vs Micrococci

1. furoxone
   - Staph
   - Micrococci
2. bacitracin: (units = _____)
   - Staph
   - Micrococci
3. lysosome: (mg disk = _____)
   - Staph
   - Micrococci
4. lysostaphin enzyme will break the cell wall
   - Staph
   - Micrococci

Answer 6

1. furoxone
   - NEG
   - POS
2. 0.04 units
   - RESISTANT
   - SUSCEPTIBLE
3. 50mg disk
   - RESISTANT
   - SUSCEPTIBLE
4. LYSOSTAPHIN TEST
   - SUSCEPTIBLE
   - RESISTANT

Question 7

Micrococci vs Staph

1. oxygen
   - strictly aerobic
   - aerobic/ facultative anaerobe
2. sugar
   - sugar oxidizer
   - sugar fermenter
3. modified oxidase reagent
   - positive result

Answer 7

1. oxygen
   - Micrococci
   - Staphylococci
2. sugar
   - Micrococci
   - Staphylococci
3. tetramethyl-paraphenylenediamine
   dihydrochloride in dimethyl sulfoxide
   - blue coloration

Question 8

SUMMARY TESTS

1. COAGULASE
   - S.aureus
   - S.saprophyticus
   - S.epidermidis
2. DNAse
   - S.aureus
   - S.saprophyticus
   - S.epidermidis
3. MANNITOL
   - S.aureus
   - S.saprophyticus
   - S.epidermidis
4. SUCROSE
   - S.aureus
   - S.saprophyticus
   - S.epidermidis
5. NOVOBIOCIN
   - S.aureus
   - S.saprophyticus
   - S.epidermidis

Answer 8

1. COAGULASE
   - POS
   - NEG
   - NEG
2. DNAse
   - POS
   - NEG
   - POS
3. MANNITOL
   - POS
   - NEG
   - NEG
4. SUCROSE
   - POS
   - POS
   - POS
5. NOVOBIOCIN
   - SUSCEPTIBLE
   - RESISTANT
   - SUSCEPTIBLE