Stains: Procedure, Principle, & Interpretation (microbiology) Flashcards
An expectorated sputum is sent to the laboratory for culture from a patient with respiratory distress. The direct specimen Gram stain shows many squamous epithelial cells and rare neutrophils. The microscopic appearance of the organisms present includes:
moderate gram-positive cocci in chains and diplocoocci
moderate gram-negative diploococci
moderate palisading gram-positive bacilli all in moderate amounts
This gram stain is most indicative of:
A) a pneumococcal pneumonia
B) an anaerobic infection
C) a Haemophilus pneumonia
D) oropharyngeal flora
D) oropharyngeal flora
Upon review of a sputum Gram stain, the technician notes that all the neutrophil nuclei in the smear stained dark blue. The best explanation for this finding is the following:
A) iodine was omitted from the staining procedure
B) slide was inadequately decolorized with acetone/alcohol
C) sputum smear was prepared too thin
D) cellular components have stained as expected
B) slide was inadequately decolorized with acetone/alcohol
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The image depicts a gram stain of a knee fluid from a patient who has recently undergone knee replacement surgery:
The best interpretation of the Gram stain is:
A) gram-positive cocci suggestive of Staphylococcus
B) gram-positive bacilli suggestive of Carynebacterium
C) gram-positive bacilli suggestive of Listeria
D) gram-positive cocci suggestive of Streptococcus
D) gram-positive cocci suggestive of Streptococcus
The principle difference between the Ziehl-Neelsen acid-fast stain technique and the Kinyoun acid-fast stain technique is the:
A) types of dyes used
B) type of microscope used to interpret stained smears
C) strength of acid decolorizer
D) use of heat to allow the dye to penetrate the organism
D) use of heat to allow the dye to penetrate the organism
Relapsing fever in humans is caused by:
A) Borrelia recurrentis
B) Brucella abortus
C) Leptospira interrogans
D) Spirillum minus
A) Borrelia recurrentis
