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BOC Book > Specimen Processing (Microbiology) > Flashcards

Specimen Processing (Microbiology) Flashcards

1
Q

A bronchoscopy sample with the request for the culture of Legionella is sent to the laboratory. The correct plating protocol is:

A) culture on thiosulfate citrate bile salt media
B) incubate the culture media anaerobically
C) reject the specimen and request a sputum sample
D) culture on buffered charcoal yeast extract agar with antibiotics

A

D) culture on buffered charcoal yeast extract agar with antibiotics

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2
Q

A community hospital microbiology laboratory is processing significant numbers of stool cultures because of an outbreak of diarrhea following heavy rains and flooding in the county. A media that should be incorporated in the plating protocol is:

A) colistin nalidixic acid for Listeria
B) MacConkey agar with sorbitol for Campylobacter
C) mannitol salt agar for Enterococcus species
D) thiosulfate citrate bile salts sucrose for Vibrio species

A

D) thiosulfate citrate bile salts sucrose for Vibrio species

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3
Q

A male urethral discharge specimen submitted for culture should be inoculated to:

A) sheep blood and phenylethyl alcohol agars
B) eosin-methylene blue and sheep blood agars
C) thioglycollate broth and chocolate agar
D) chocolate and modified Thayer-Martin agars

A

D) chocolate and modified Thayer-Martin agars

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4
Q

The steam autoclave method of sterilization requires:

A) 15 lb of pressure for 15 mins
B) dry heat for 20 mins
C) a maximum temperature of 100 degrees C
D) a source of ethylene oxide

A

A) 15 lb of pressure for 15 mins

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5
Q

Which selective medium is used for the isolation of gram-positive microorganisms?

A) Columbia CNA with 5% sheep blood
B) trypticase soy agar with 5% sheep blood
C) eosin methylene blue
D) modified Thayer-Martin

A

A) Columbia CNA with 5% sheep blood

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6
Q

Campylobacter jejuni isolation requires the fecal specimen to be:

A) inoculated onto selective plating media and incubated in reduced oxygen with added CO2 at 42 degrees C
B) stored in tryptic soy broth before plating to ensure growth of the organism
C) inoculated onto selective plating media and incubated at both 35 degrees C and at room temp
D) incubated at 35 degrees C for 2 hrs in Cary-Blair media before inoculating onto selective plating media

A

A) inoculated onto selective plating media and incubated in reduced oxygen with added CO2 at 42 degrees C

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7
Q

Which of the following specimen requests is acceptable?

A) feces submitted for anaerobic culture
B) Foley catheter tip submitted for aerobic culture
C) rectal swab submitted for direct smear for gonococci
D) urine for culture of acid-fast bacilli

A

D) urine for culture of acid-fast bacilli

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8
Q

A cerebrospinal fluid specimen containing only 2 drops of CSF was collected by a lumbar puncture from a febrile 25 year old male and was submitted for a stat gram stain and culture. The direct specimen Gram stain was reported as many neutrophils and no microorganisms seen. The remaining drop of CSF should be inoculated to:

A) blood agar
B) CNA agar
C) chocolate agar
D) Thayer-Martin agar

A

C) chocolate agar

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9
Q

A diabetic foot swab from an 82-year-old woman with recurrent infections is submitted for culture. The Gram stain reveals

many neutrophils, no squamous epithelial cells
many gram neg bacilli
many gram pos cocci in chains

The physician requests that all pathogens be worked up. In addition to the sheep blood, chocolate and MacConky agar plates routinely used for wound cultures, the technologist might also process a(n):

A) anaerobic blood agar plate
B) BCYE agar plate
C) CNA agar plate
D) XLD agar plate

A

C) CNA agar plate

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10
Q

Which of the following is the most appropriate specimen source and primary media selection?

A) CSF Clumbia CNA, MacConkey
B) endocervial chocolate, Martin Lewis
C) sputm sheep blood, Thayer-Martin, KV-laked blood
D) urine sheep blood, chocolate, Columbia CNA

A

B) endocervial chocolate, Martin Lewis

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11
Q

Which of the following is the most appropriate organism and media combination?

A) Vibrio species - Skirrow
B) Enterohemorrhagic E. coli - phenylethyl alcohol (PEA)
C) Campylobacter species - charcoal yeast extract
D) Yersina enterocolitica - cefsulodin-irgasan-novoiocin (CIN)

A

D) Yersina enterocolitica - cefsulodin-irgasan-novoiocin (CIN)

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12
Q

A Gram stain from a swab of a hand wound reveals:

moderate neutrophils
no squamous epithelial cells
moderate gram-positive cocci in clusters
moderate large gram-negative bacilli

Select the appropriate media that will selectively isolate each organism:

A) KV-laked agar, Thayer-Martin
B) sheep blood, MacConkey
C) Columbia CNA, Chocolate
D) Colubmia CNA, MacConkey

A

D) Colubmia CNA, MacConkey

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13
Q

An organism that must be incubated in a microaerophilic environment for optimal recovery is:

A) campylobacter jejuni
B) Escherichia coli
C) Pseudomonas aeruginosa
D) Proteus mirabillis

A

A) campylobacter jejuni

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14
Q

Vibrio parahaemolyticus is best isolated from feces on:

A) eosin methylene blue (EMB) agar
B) Hektoen enteric (HE) agar
C) Salmonella Shigella (SS) agar
D) thiosulfate citrate bile salts (TCBS) agar

A

D) thiosulfate citrate bile salts (TCBS) agar

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15
Q

Media used to support growth of Legionella pneumophila should contain the additives:

A) X and V factors
B) hemin and vitamin k
C) charcoal and yeast extracts
D) dextrose and laked blood

A

C) charcoal and yeast extracts

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16
Q

The best medium for culture of Bordetella pertussis is:

A) phenylethyl alcohol agar
B) potassium telluride blood agar
C) Regan-Lowe agar
D) Tinsdale agar

A

C) Regan-Lowe agar

17
Q

A medium that can be used to recover francisella tularensis is:

A) Bordet-Gengou agar
B) Buffered charcoal yeast extract agar
C) Loeffler agar
D) MacConkey agar

A

B) Buffered charcoal yeast extract agar

18
Q

A 21 year old patient presents with pharyngitis. A throat swab is collected and submitted for anaerobic culture. This specimen should be:

A) set up immediately
B) rejected as unacceptable
C) inoculated into thioglycollate broth
D) sent to a reference laboratory

A

B) rejected as unacceptable

19
Q

An antibiotic used to suppress or kill contaminating fungi in media is:

A) amphotericin B penicillin
B) chloramphenicol
C) cycloheximide
D) streptomycin

A

C) cycloheximide

20
Q

27

A sputum specimen is recieved for culture and Gram stain. The Gram-stained smear from this specimen is seen in the image
The technologist’s best course of action would be to:

A) inoculate appropriate media and incubate anaerobically
B) inoculate appropriate media and incubate aerobically
C) call the physician and notify his of this “life-threatening” situation
D) call the patient care area and request a new specimen

A

D) call the patient care area and request a new specimen

21
Q

A vaginal/rectal swab is collected from a pregnant patient to screen for group B Streptococcus colonization. The best medium to inoculate the specimen to is:

A) CNA agar
B) LIM broth
C) sheep blood agar
D) thioglycollate broth

A

B) LIM broth

22
Q

When processing a patient specimen for Gram stain and culture, the proper use of a biological safety cabinet includes:

A) bringing into the cabinet all required media and equipment just prior to setting up each individual specimen
B) keeping the ultraviolet light on for the first 30 mins of working in the cabinet
C) not using any heat-generating equipment such as open flames or micro burner/incinerators
D) not disrupting the air curtain barrier by keeping airflow and exhaust grills unobstructed

A

D) not disrupting the air curtain barrier by keeping airflow and exhaust grills unobstructed

23
Q

A wound specimen grew 2 colony types on sheep blood agar and a single, clear colony type on MacConkey agar. Sheep blood agar growth was documented as:

colony type #1: swarming over entire plate, Gram stain: gram-negative bacilli
colony type #2: white colony, Gram-stain: gram-positive cocci in clusters

The best way to isolate colony type #2 from colony type #1 is to subculture:

A) colony #1 to sheep blood and chocolate agars
B) colony #1 to sheep blood and/or MacConkey agar
C) colony #2 to sheep blood and chocolate agars
D) colony #2 to CNA and/or PEA agar

A

D) colony #2 to CNA and/or PEA agar

24
Q

A differential medium that can be used as a primary isolation agar producing predictable colored colonies that can be distinguished from other organism colony types describes:

A) buffered charcoal yeast extract agar
B) blood phenylethyl alcohol agar
C) camylobacter blood agar
D) chromagar

A

D) chromagar

25
Q

Anticoagulants acceptable for use with blood, bone marrow and synovial fluid specimens that are to be cultured include:

A) EDTA and sodium citrate
B) heparin and sodium citrate
C) sodium polyanethol sulfonate (SPS) and heparin
D) sodium polyanethol sulfonate (SPS) and EDTA

A

C) sodium polyanethol sulfonate (SPS) and heparin

26
Q

Appropriate culture requirements for a specimen from a patient suspected of having tularemia include:

A) a media with cysteine such as buffered charcoal yeast extract agar
B) colistin nalidixic acid agar
C) Mueller-Hinton agar with 5% sheep blood agar
D) Regan-Lowe media

A

A) a media with cysteine such as buffered charcoal yeast extract agar

27
Q

The primary isolation of Neisseria gonorrhoeae requires:

A) anaerobic conditions
B) starch media
C) carbon dioxide
D) blood agar

A

C) carbon dioxide

28
Q

Acceptable specimen sources for culture of anaerobic bacteria includes:

A) abscess
B) stool
C) clean catch urine
D) vaginal

A

A) abscess

29
Q

In general, anaerobic infections differ from aerobic infections in which one of the following?

A) they usually respond favorably to aminoglycodise therapy
B) they usually arise from exogenous sources
C) they are usually polymicrobic
D) Gram stains of specimens are less helpful in diagnosis

A

C) they are usually polymicrobic

BOC Book (18 decks)

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