Stains Flashcards
Haematoxylin & Eosin
A) Nuclei,
B) Cytoplasm & collagen
C) Membranes, muscle, keratin
D) Erythrocytes
Blue
Pale Pink
Bright pink
Orange red
What does Masson Trichrome stain
Connective tissue esp. collagen
Masson Trichrome staining
A) Nuclei
B) Muscle, epithelia
C) collagen
D) red blood cells
Blue (Hx)
Pink (Ponceau red)
Green (light green)
Scarlet (Acid Fuchsin)
What does Verhoeffs Van Gieson stain?
Elastin fibers
E.g in arteries, lungs, skin
Verhoeffs Van Gieson
A) Elastin fibres, nuclei
B) Cytoplasm, muscle
C) Collagan
Black
Yellow
Red
What does Gordan & Sweets Silver stain?
Reticular fibres (Collagen type III) - in liver, spleen, lymph nodes.
Many in basement membrane.
Gordan & Sweets Silver stain
A) Reticulin
B) Background
Black
Green.
Is G&S Stain argyrophillic or argentaffin stain?
Argyrophillic
What does Perls Prussian Blue stain?
Iron deposits (hemosiderin) - commonly used in liver, bone marrow and spleen samples.
Perls Prussian Blue
A) Iron deposits (hemosiderin)
B) Background
Blue
Pink/Red
What does Alcian Blue/Periodic Acid Schiff stain?
Glycogen, neutral and acid mucins.
Alcian Blue/Periodic Acid Schiff
A) Acidic mucins (glycoaminoglycans)
B) Neutral mucins/glycogen/basement membrane/fungi
C) Nuclei
Sky Blue
Magenta (PAS)
Deep Blue
What does Congo Red stain?
Amyloid deposits
Congo red
A) Amyloid deposits
B) Nuclei
Pink-red
Blue
What does Grocott’s Methenamine Silver stain?
Fungi - Pneumocystis, Candida
Grocott’s Methenamine Silver
A) Fungal cell walls
B) Background
Black
Green
where are acid mucins found
Goblet cells.
Villi are lined with enterocytes (columnar epithelial cells) and interspersed are goblet cells.
Loads of goblet cells in crypts of Lieberkuhn
Where are neutral mucins found
Brush border and basement membrane of intestines.
Where is iron deposited in liver?
Hepatocytes - granular
Kuppfer cells
Located near portal triads
Where does amyloid accumulate in kidneys?
In renal amyloidosis -
1. Glomeruli (causes nephrotic syndrome due to severe proteinuria, hypoalbuminemia, edema)
2. Blood vessel walls (arteries + arterioles) can contribute to hypertension + chronic kidney disease
3. tubular basement membrane - PCT + DCTs - leads to tubular atrophy.
