Stain components

Question 1

Haematoxylin & Eosin

Answer 1

1. Haematoxylin with alum
2. 1% Acid alcohol (1% HCl in spirit)
3. Tapwater - to blue
4. Eosin

Question 2

Masson trichrome

Answer 2

1. Celestine blue + Mayers Htx
2. Tap water to blue
3. Ponceau red/ Acid Fuchsin solution
4. 1% Phosphomolybdic acid
5. 0.1% Light green

Question 3

Verhoeffs van Gieson components

Answer 3

1. Verhoeffs dye
   
   • Haematoxylin
   • Ferric chloride
   • Verhoeff’s Iodine
2. Ferric chloride to differentiate
3. Water to differentiate,
4. Van gieson stain (picric acid + acid fuchsin)

Question 4

Gordan and Sweets stain components`

Answer 4

1. Potassium permanganate
2. Oxalic acid
3. Iron alum
4. Silver stain
5. 10% Formalin
6. Gold chloride
7. Sodium thioshulphate
8. Light green

Question 5

Perls Prussian Blue stain components

Answer 5

1. Perls solution:
   
   • HCl
   • Potassium ferrocyanide
2. 1% Eosin

Question 6

Alcian Blue/Periodic Acid Schiff stain components

Answer 6

1. Alcian blue - pH2.5
2. 1% Periodic acid
3. Schiff reagent (basic fuchsin)
4. Mayers Haematoxylin
5. Tap water to blue/

Question 7

Congo Red

Answer 7

1. Congo red in 50% alcohol
2. Potassium hydroxide in 80% alcohol
3. Mayers haematoxylin
4. Tap water to blue.

Question 8

Grocotts Methenamine Silver stain

Answer 8

1. 5% Chromic acid
2. Silver stain
3. Gold chloride
4. Sodium thiosulphate
5. Light green