Verhoeffs van Gieson stain Flashcards
What is VVG?
A two-part combination stain that enables differentiation of some connective tissue components in a tissue which are not easily distinguished by H&E staining.
What does the Verhoeff stain component specifically target?
Elastic fibers.
What type of bonds does the Verhoeff stain form with elastin?
Hydrogen bonds and ionic interactions.
Elastin fibres stain black.
Which tissues primarily contain elastin?
- Lung
- Arteries
- Skin
What is the purpose of the van Gieson stain component?
It is a counterstain.
What are the two acid dyes that make up Van Gieson’s stain?
- Picric acid
- Acid fuchsin
What type of proteins do Van Gieson’s stain bind to?
Basic proteins in tissues.
acid fuchsin binds to Strongly acidic collagen -> red.
Picric acid binds to less acidic muscle -> yellow.
Which dye in Van Gieson’s stain is a larger molecule?
Acid fuchsin.
Which components does picric acid stain?
- Red blood cells
- Muscle
- Cytoplasm
dyes Yellow
Fill in the blank: VVG is used to differentiate connective tissue components that are not easily distinguished by _______.
H&E staining.
VVG staining
Nuclei - black
Elastin- black
Muscle, RBCs, Cytoplasm - yellow
Collagen - red
What does acid fuchsin stain?
Collagen
What is the purpose of ferric chloride in Verhoeffs haematoxylin?
Mordant + oxidizer - helps binds haematoxylin to elastic fibres.
What is the function of iodine in verhoeffs haematoxylin?
Oxidant - converts haematoxylin to haematin.
Mordant = enhances staining by facilitating binding of dye to tissue.
Dye trapping agent - prevents dye loss from selected components during the subsequent differentiation process.
What is Verhoeffs haematoxylin complexed with?
Iodine and ferric chloride mordants.
Both are also oxidizers.
Iodine is a dye-trapping agent.
Ferric chloride used as differentiating agent.
WHat is used for differentiation?
Ferric chloride.
