stains

Question 1

oldest stain

stains amyloid

Answer 1

iodine

Question 2

most valuable stain

used as a counterstain

Answer 2

eosin

Question 3

common basic nuclear stain

Answer 3

methylene blue

Question 4

metachromatic; stains leukocytes

Answer 4

methylene violet

Question 5

simplest method of differential staining of collagen

demonstrate connective tissue

Answer 5

Van Gieson

Question 6

stains blood + differentiate leukocytes

Answer 6

Giemsa

Question 7

both a decolorizer and counterstain

stains Ascaris eggs, RBCs, bacterial spore

Answer 7

Malachite green

Question 8

most reliable and specific for DNA

Answer 8

Feulgen’s stain

Question 9

stains calcium (black)

Answer 9

Von Kossa Silver Nitrate

Question 10

fixative and stain

demonstrate connective tissue

Answer 10

picric acid

Question 11

chromatin stain for fresh materials in smear preparations

Answer 11

carmine

Question 12

best vital stain

stains cell granules and vacuole phagocytic cells

Answer 12

neutral red

Question 13

demonstrate mitochondria in frozen sections

Answer 13

Janus Green B

Question 14

demonstrate neuroglia in frozen sections

Answer 14

victoria blue

Question 15

most sensitive of all fat dyes

phospholipids

Answer 15

sudan black

Question 16

stains triglycerides and neutral lipids (deep red)

fat dye

Answer 16

sudan black IV

scharlach R

Question 17

stains fat orange

Answer 17

Sudan III

Question 18

fixative and stain for fats

Answer 18

osmic acid/osmium tetroxide stain

Question 19

stains melanin (black)

metallic impregnation stain

Answer 19

masson fontana stain

Question 20

stains hemosiderin

Answer 20

perl’s prussian blue

Question 22

fluorescence stain

Answer 22

auramine rhodamine

Question 23

progressive stain for RES

Answer 23

tryphan blue

Question 24

serves a link/bridge between the tissue and the dye to make staining reaction possible

Answer 24

mordant

Question 25

# staining method: stain is intensified by a mordant and accentuator

Answer 25

indirect stainins

Question 26

# staining method: uses simple solutions or aqeous solutions or alcoholic dye solutions

Answer 26

direct staining

Question 27

# staining method: tissue elements are **stained in a definite sequence**, and the staining solution is applied for specific periods of time or until the desired intensity of coloring of the different tissue elements is attained

Answer 27

progressive

Question 28

# staining method: tissue is first **overstained** to obliterate the cellular details, and the excess stain is removed or **decolorized** from unwanted parts of the tissue, until the desired intensity of color is obtained

Answer 28

regressive staining

Question 29

# staining method: use of specific dyes which **differentiate** particular substance by staining them with a color that is different from that of the stain itself. (*Orthochromatic* – color is similar to the dye)

Answer 29

metachromic staining

Question 30

# staining method: application of a different color or stain to **provide contrast and background** to the staining of the structural components to be demonstrated

Answer 30

counterstain

Question 31

# staining method tissue elements are demonstrated by **COLORLESS solutions of metallic salts** which are thereby reduced by the tissue | result: opaque, black deposits

Answer 31

metallic impregnation

Question 32

# staining method: **selective** staining of LIVING cell constituents, demonstrating cytoplasmic structures by phagocytosis of the dye particle | best: **neutral red** ## Footnote not demonstrated: nucleus (resistant living cell)

Answer 32

vital staining

Question 33

# staining method: **injecting** dye into any part of the animal body (intravenous, intraperitoneal or subcutaneous) | india ink

Answer 33

intravital stain

Question 34

# staining method: staining of living cells immediately after removal from the living body

Answer 34

supravital staining | neutral red