Sectioning and Microtomy Flashcards

1
Q

Excess wax is cut off from the block to expose the tissue surface in preparation for actual section cutting.

A

Trimming

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2
Q

A process by which tissue is cut into uniformly thin slices or sections on a precision instrument called a “microtome” using extremely fine steel blades.

A

Sectioning

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3
Q

cutting engine of the histopath lab

A

microtome

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4
Q

Means by which tissues can be sectioned and attached to the surface so that examination by microscopy can take place.

A

Microtomy

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5
Q

What microtome knife is designed for ultrathin section microtome examined under an electron microscope?

A

Diamond edged knife

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6
Q

What microtome knife is designed for rotary microtomes?

A

Biconcave knife

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7
Q

What is the most commonly used microtome?

A

Rotary microtome

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8
Q

What microtome knife is used for rotary and rocking, celloidin embedded microtome and one side is flat the other side is concave?

A

Plane-concave knife

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9
Q
  • removal of gross nicks and blemishes, and grinding the cutting edge of the knife to stone
  • lubrication: xylene, mineral oil, clove oil, paraffin, and soapy water
  • “Edge first” heel to toe, 20-30 times, abrasives (powdered aluminum oxide).
A

Honing

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10
Q
  • removal of the burr
  • “Edge last” Toe to heel, 40-120 double strokes
  • Horse leather (use castor oil to prevent rusting)
A

Stropping

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11
Q

Identify the material for honing.

  • Best manual sharpening
A

Belgium Yellow

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12
Q

Identify the material for honing.

  • more polishing effect than Belgium Yellow
A

Arkansas

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13
Q

Identify the material for honing.

  • much coarser for badly nicked knives
A

Fine carborundum

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14
Q

Identify the material for honing.

  • use fiamanthine for final polishing
A

Plate glass hone

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15
Q

Identify the material for honing.

  • glass disc or wheel driven by electric motor
A

Machine hone

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16
Q

What is the angle for the bevel of the knife?

A

27-32 degrees

17
Q

What is the clearance angle when using a knife?

A

5-15 degrees

18
Q

Identify the type of microtome.

  • for cutting serial sections of large blocks of paraffin-embedded tissues
  • cuts section in a slightly curved plane
  • simplest and oldest
  • vibrates while cutting
  • 10-12microns
  • Caldwell &Trefall 1881
A

Rocking microtome

19
Q

Identify the type of microtome.

  • for cutting paraffin-embedded tissues in a flat plane
  • no vibrations
  • 4 to 6 µm
  • Minot
A

Rotary microtome

20
Q

Identify the type of microtome.

  • for cutting celloidin-embedded tissues
  • 7-9 µm
A

Sliding microtome

21
Q

Identify the type of microtome.

  • used for electron microscopy
  • 0.5 microns
A

Ultra-thin microtome