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Histopathology > Staining > Flashcards

Staining Flashcards

1
Q

Factor that influence the staining process that certain part of cells and tissues that are acidic in character have greater affinity for the basic dyes ,while the basic take more of the acid stains

A

Chemical

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2
Q

2 factors that influence the staining process

A

Physical phenomenon of capillary osmosis

Chemical

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3
Q

Is the process of applying dyed on the sections to see and study the architectural patter of the tissue and physical characteristics of the cell

A

Staining

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4
Q

Process by which sections are stained with simple aqueous soln or alcoholic soln.

A

Direct staining

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5
Q

Process of staining the tissue sections which requires the use of mordant

A

Indirect staining

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6
Q

Used in staining to enhance the O2 binding capacity to the tissue by the process of oxidation

A

Oxidizing agent

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7
Q

Rgt. Used in staining to diminish the O2 binding capacity to the tissue by the process of reduction

A

Reduction rgt.

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8
Q

Example of Reducing Agent

A

Pyrogallic acid , hydroquinone

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9
Q

Total removal of color previously imparted o tissues

give example

A

Bleaching , oxalic acid

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10
Q

Chemical agent which heighten the color intensity crispness and selectivity of the stain.

A

Accentuator

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11
Q

There is a continuous staining of the tissue sections until the desired intensity color is obtained

A

Progressive staining

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12
Q

The tissue is first overstrained and then the excess stain is later removed by another rgt. Thus differentiating staining of tissue details

A

Regressive staining

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13
Q

Process of applying less or exposing tissue to dyes for shorter period of time

A

Understaining

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14
Q

Process of applying dye in excess or of exposing the tissue to dye in longer period of time

A

Overstaining

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15
Q

The application of a diff color stain to provide contrast and back ground to the stain of the structural components to be demonstrated

A

Counter staining

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16
Q

The ability of the dye to produce one color only

A

Monochromasia

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17
Q

The ability of the dye to produce different color to the tissue

A

Polychromasia

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18
Q

Process in which a dye forms other dyes spontaneously by virtue of chemical rxn. Which take place such as in the oxidation of methylene blue in soln upon standing resulting of 2 dyes homologous Azure A and B

A

Polychroming

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19
Q

The ability of the dye to produce different colors of different tinge or hues to the elements of the tissue

A

Metachroming

20
Q

Used to demo star the general relationship of tissue and cells with general differentiation of nucleus and cytoplasm w/o necessarily emphasizing the inclusion bodies

A

Micro anatomical staining

21
Q

Process of staining the living body

A

Vital staining

22
Q

Demonstrate minute specific structure in cytoplasm and nucleus without necessarily differentiating tissue in general

A

Cytoplasmic staining

23
Q

Used to demonstrate bacterial morphology . The substance and the organism are mixed on the slide and examined microscopically,the unstained organism a black background

A

Negative staining

24
Q

Process of coating slides with dilute celloiden soln. For greater adherence of sections to slide so they will not detached off during stain

A

Collodioniation

25
Q

Process of injecting the dye or stain to the living body

A

Intravital staining

26
Q

Process of staining the tissue sections with a little differentiation except between the nucleus and cytoplasm

A

Routine staining

27
Q

Group of benzene ring which can impart net change to the molecule by conferring the properly of electrolyte dissociation

A

Auxochrome

28
Q

Substance that passes chromophores grp of benzene ring which confers color and alter light resonance properties

A

Chromagen

29
Q

Demonstrate minute specific structure found in the cytoplasm and nucleus without necessarily differentiate tissue structural in general

A

Substantive

30
Q

Physical phenomenon of capillary osmosis

A

S.A.A

31
Q

Dyes are divided in 3 Categories

A

S.I.N ( Natural Dyes , InOrganic Dye , Synthethic Dye)

32
Q

4 types of Natural Dyes

A

Hematoxylin , Orcein , Carmine , Saffron

33
Q

Purpose of Staining

A

To enable to study and see the physical characteristic of tissue as well as their cell constituent.
To identify the different tissue components through there color reaction
To increase the optical density of tissue for better microscopic study

34
Q

3 types of Iron Hematoxylin

A

Weigert , Haidenhain Hematoxylin , Phospotungstic acid Hematoxylin

35
Q

Type of Iron hematoxylin use for demonstrating muscle fiber and connective tissue

A

Weigert’s

36
Q

Type of Iron hematoxylin recommended for regressive staining

A

Haidenhain hematoxylin

37
Q

Type of Iron hematoxylin usually use in progressive

A

Phosphotungstic Acid Hematoxylin

38
Q

4 types of Hematoxylin

A

Mayers Hematoxylin , Ehrlich Hematoxylin , Coles Hematoxylin , Harris Hematoxylin

39
Q

Type of Hematoxylin generally used for regressive staining differentiated with 1 % HCL in 70 % acid alcohol until nucleus is selectively stained

A

Ehrlich

40
Q

Type of Hematoxylin chemically ripened with sodium ioadate . It can be used as regressive stain but it is also useful as a progressive stain

A

Mayer

41
Q

Type of Hematoxylin recommended for routine purporse , especially used in sequence with Celestine Blue

A

Coles’s

42
Q

Type of Hematoxylin that is a good regressive stain that may either be used immediately or stored for future use

A

Harris

43
Q

Are substance with definite atomic groupings and are capable of producing visible colors

A

Chromophore

44
Q

An auxiliary radical or substance which imparts to the cpd the property of electrolytic dissociation , thereby altering the shade of the dye , enabling it to form salts with another cpd and ultimately retaining its color

A

Auxochrome

45
Q

Hematoxylin is a natural dye that comes from the extract ether of what tree

A

H. Campechainum

46
Q

Cochinial name of the Bug

A

Coccus cacti

47
Q

Saffron name is natural pigment extracted by the stomata of

A

Croccussativous

Histopathology (8 decks)

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