Special Stain Flashcards
The simplest method of differential staining of collagen
Van Gleson’s stain
The collagen color in van Gleson’s stain
Pink or deep red
Excellent and colorful method of demonstrating connective tissue fiber
Mallory’s aniline blue stain
Enumerate 4 special stain use in collagen
Van Gleson’s
Mason trichrome stain
Mallory aniline blue stain
Azocarmine stain
Enumerate the 4 elastic fiber stain
Weigert’s Elastic Tissue stain
Taenzer - Unna Orcein method
Krajian technique
Verhoeff stain
Elastic fiber appear dark blue or blue black
Weigert’s Elastic Tissue stain
Excellent stain for elastic fibers especially in dermatology
Taenzer- Unna Orcein Method
Rapid method of elasting staining
Krajian technique
Enumerate the 5 Reticulum connective tissue Fiber stain
Silver impregnation technique PAS Bielschowsky's method PALFT Gold method
Best stain for reticulum
Silver impregnation technique
In reticulum connective tissue fiber stains PAS stains reticulum in what color?
Purplish red
Give the special stain use in carbohydrates and connective tissue ground substance
Carbohydrate Glycogen Acid mucopolysaccharide Amyloid Basement membrain stain
Is a histochemical stain that will demonstrate carbohydrates with the use of periodic acid . Give also the positive color of the substance
PAS . Red or magenta red
Are the most common PAS positive substance
Mucoproteins
Oldest stain and obsolete
Langhann ‘s iodine
Give the 3 glycogen stains
PAS
Best carmine method
Langhann’s iodine
Selective and highly specific for glycogen
Best carmine method
Widely used for demonstration of glycogen
PAS
Give the 3 Acid mucopolysaccharide stain
Metachromic stain
Gomorri’s Aldehyde Fuchsin stain
Fluorescent Acridine Orange
Specific for acid mucopolysaccharide
Gomorri’s Aldehyde Fuchsin stain
Give a selective brilliant orange fluorescence
Fluorescent Acridine Orange Technique
Give the 5 amyloid stains
Gram Iodine Krajan Amyloid Congo Red method Metachromatic staining Induce fluorescent staining with Thioflavine - T
Turn amyloid deep blue
Gram’s Iodine stain
A test for brilliant staining of amyloid. Give the positive color
Krajan’s amyloid stain .Red
Highly selective stain for amyloid giving deep pink or red color
Congo red method
Stain use in amyloid and mast cell . Give the positive color
Induces Fluorescent Staining Thioflavine- T . yellow fluorescence
Most common method for the demonstration of basement membrane
PAS
Give the 5 muscle and bone stains
H & E stain Masson trichrome stain Van Gleson's stain Mallory's Phospotungstic Acid Hematoxylin ( PTAH ) Lissamine fast red
Routine method for bone staining
H & E stain
Stain muscle yellow
Van Gleson’s stain
Show striation in skeletal and cardiac muscle fibers , Collagen . Give the positive color for Collagen
Mallory Phospotungstic Acid Hematoxylin stain
Collagen is yellow, muscle striation in skeletal and cardiac muscle fibers , Collagen is reddish
Lissamine Fast red
It is the routine staining method of choice for bone histology
Careful differentiation with Heidenhain’s iron hematoxylin and more easily with eosin as a counter stain
4 special stains use in the Nervous system
Nissl Bodies
Nerve cell bodies/Neurons
Neuroglis stain
Myelin sheath
Enumerate the 4 Nssl Bodies stain
H&E
Thionine
Toluidine Blue
Methylene Blue
Nssl bodies is also known as
Tigroid aubstance
Nssl bodies stain that the color of the positive is Violet
Thionine
Enumerate the 6 nerve cell bodies
H&E hematoxylin and Van Gleson's and neurofibromas Mallory PTAH overnight staining in dilute giemsa Bielschowsy Technique Cajal gold sublimate method
Excellent stains for astrocytes
Mallory’s PTAH
Stains astrocytes black and nerve cell red
Cajal Gold Sublimate method
The only stain use in Neuroglia stain
Silver impregnation technique
Enumerate 3 myelin sheath stain
Osmic Acid Technique
Weigert’s Pal tech
Sudan black method
Stains myelin sheath black
Osmic acid tech
Myelin sheath blue black stain
Weigert’s Pal technique
Rapid staining of myelin
Sudan black method
Most sensitive lipid stain
Sudan black b
The most commonly used stain producing a rapid permanent colorization of lipid
Sudan IV
Other name for Sudan IV
Scharlach R
Stains neutral fats and lipifuschin
Oil Red O
Stains fats black
OAS
Special stain for fats only
Nile blue sulfate
Excessive quantity and abnormal location of normal body pigments
Endogenous pigmentations
6 kinds of Endogenous pigments
Hemosiderine Hematoidin Hematin Hemozoin Hemofuscin Melanin
Iron containing pigments of hemoglobin
Hemosiderine
Hemosiderine can be stained with
Prussians blue
Turn Bulls blue
The iron free pigment of hemoglobin
Hematoidin
Hematoidin can be stained with
Gmelins test
Stein iodine test
Hemoglobin minus the globin molecule
Hematin
Black granules formed by malarial parasites
Hemozoin
Iron free brownish yellow pigment which occurs with Hemosiderin on hemochromatosis
Hemofuscin
Brown or black pigments normally found in the skin and eyes
Melanin
Technique use for melanin pigments
Masson Fontana technique
Give examples of exogenous pigments
Tattoo pigments Asbestos Carbon Silica Iron Silver
Enumerate the 3 artifacts pigments
Formaldehyde deposit
Mercuric chloride deposit
Osmium tetroxide deposits
Is particularly recommended for staining skeletal system in embryos and fetuses
Calcium dye lake reaction
2 stain used for bacteria
Acid fast stain
Gram stain
2stain use in spirochete
Rapid giemsa
Levaditi method
Fungi stain
Hematoxylin Gram staining Giemsa stain Ziehl Neelsen's Method Metachromasia of capsule
4 Special stains use for microorganism
Hitchkiss - McManus PAS technique
Bauer feulgen technique
Alicante blue method
Gridley stain for fungi
Give the 4 special stain use in Connective tissue
Collagen
Elastic fibers
Reticulum connective tissue fiber stains
