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Histopathology > Special Stain > Flashcards

Special Stain Flashcards

1
Q

The simplest method of differential staining of collagen

A

Van Gleson’s stain

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2
Q

The collagen color in van Gleson’s stain

A

Pink or deep red

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3
Q

Excellent and colorful method of demonstrating connective tissue fiber

A

Mallory’s aniline blue stain

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4
Q

Enumerate 4 special stain use in collagen

A

Van Gleson’s
Mason trichrome stain
Mallory aniline blue stain
Azocarmine stain

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5
Q

Enumerate the 4 elastic fiber stain

A

Weigert’s Elastic Tissue stain
Taenzer - Unna Orcein method
Krajian technique
Verhoeff stain

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6
Q

Elastic fiber appear dark blue or blue black

A

Weigert’s Elastic Tissue stain

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7
Q

Excellent stain for elastic fibers especially in dermatology

A

Taenzer- Unna Orcein Method

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8
Q

Rapid method of elasting staining

A

Krajian technique

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9
Q

Enumerate the 5 Reticulum connective tissue Fiber stain

A 
Silver impregnation technique
PAS 
Bielschowsky's method
PALFT
Gold method
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10
Q

Best stain for reticulum

A

Silver impregnation technique

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11
Q

In reticulum connective tissue fiber stains PAS stains reticulum in what color?

A

Purplish red

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12
Q

Give the special stain use in carbohydrates and connective tissue ground substance

A 
Carbohydrate
Glycogen
Acid mucopolysaccharide 
Amyloid
Basement membrain stain
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13
Q

Is a histochemical stain that will demonstrate carbohydrates with the use of periodic acid . Give also the positive color of the substance

A

PAS . Red or magenta red

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14
Q

Are the most common PAS positive substance

A

Mucoproteins

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15
Q

Oldest stain and obsolete

A

Langhann ‘s iodine

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16
Q

Give the 3 glycogen stains

A

PAS
Best carmine method
Langhann’s iodine

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17
Q

Selective and highly specific for glycogen

A

Best carmine method

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18
Q

Widely used for demonstration of glycogen

A

PAS

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19
Q

Give the 3 Acid mucopolysaccharide stain

A

Metachromic stain
Gomorri’s Aldehyde Fuchsin stain
Fluorescent Acridine Orange

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20
Q

Specific for acid mucopolysaccharide

A

Gomorri’s Aldehyde Fuchsin stain

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21
Q

Give a selective brilliant orange fluorescence

A

Fluorescent Acridine Orange Technique

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22
Q

Give the 5 amyloid stains

A 
Gram Iodine
Krajan Amyloid
Congo Red method
Metachromatic staining
Induce fluorescent staining with Thioflavine - T
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23
Q

Turn amyloid deep blue

A

Gram’s Iodine stain

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24
Q

A test for brilliant staining of amyloid. Give the positive color

A

Krajan’s amyloid stain .Red

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25
Q

Highly selective stain for amyloid giving deep pink or red color

A

Congo red method

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26
Q

Stain use in amyloid and mast cell . Give the positive color

A

Induces Fluorescent Staining Thioflavine- T . yellow fluorescence

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27
Q

Most common method for the demonstration of basement membrane

A

PAS

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28
Q

Give the 5 muscle and bone stains

A 
H & E stain
Masson trichrome stain
Van Gleson's stain
Mallory's Phospotungstic Acid Hematoxylin ( PTAH )
Lissamine fast red
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29
Q

Routine method for bone staining

A

H & E stain

30
Q

Stain muscle yellow

A

Van Gleson’s stain

31
Q

Show striation in skeletal and cardiac muscle fibers , Collagen . Give the positive color for Collagen

A

Mallory Phospotungstic Acid Hematoxylin stain

32
Q

Collagen is yellow, muscle striation in skeletal and cardiac muscle fibers , Collagen is reddish

A

Lissamine Fast red

33
Q

It is the routine staining method of choice for bone histology

A

Careful differentiation with Heidenhain’s iron hematoxylin and more easily with eosin as a counter stain

34
Q

4 special stains use in the Nervous system

A

Nissl Bodies
Nerve cell bodies/Neurons
Neuroglis stain
Myelin sheath

35
Q

Enumerate the 4 Nssl Bodies stain

A

H&E
Thionine
Toluidine Blue
Methylene Blue

36
Q

Nssl bodies is also known as

A

Tigroid aubstance

37
Q

Nssl bodies stain that the color of the positive is Violet

A

Thionine

38
Q

Enumerate the 6 nerve cell bodies

A 
H&E
hematoxylin and Van Gleson's and neurofibromas
Mallory PTAH
overnight staining in dilute giemsa
Bielschowsy Technique
Cajal gold sublimate method
39
Q

Excellent stains for astrocytes

A

Mallory’s PTAH

40
Q

Stains astrocytes black and nerve cell red

A

Cajal Gold Sublimate method

41
Q

The only stain use in Neuroglia stain

A

Silver impregnation technique

42
Q

Enumerate 3 myelin sheath stain

A

Osmic Acid Technique
Weigert’s Pal tech
Sudan black method

43
Q

Stains myelin sheath black

A

Osmic acid tech

44
Q

Myelin sheath blue black stain

A

Weigert’s Pal technique

45
Q

Rapid staining of myelin

A

Sudan black method

46
Q

Most sensitive lipid stain

A

Sudan black b

47
Q

The most commonly used stain producing a rapid permanent colorization of lipid

A

Sudan IV

48
Q

Other name for Sudan IV

A

Scharlach R

49
Q

Stains neutral fats and lipifuschin

A

Oil Red O

50
Q

Stains fats black

A

OAS

51
Q

Special stain for fats only

A

Nile blue sulfate

52
Q

Excessive quantity and abnormal location of normal body pigments

A

Endogenous pigmentations

53
Q

6 kinds of Endogenous pigments

A 
Hemosiderine
Hematoidin
Hematin
Hemozoin
Hemofuscin
Melanin
54
Q

Iron containing pigments of hemoglobin

A

Hemosiderine

55
Q

Hemosiderine can be stained with

A

Prussians blue

Turn Bulls blue

56
Q

The iron free pigment of hemoglobin

A

Hematoidin

57
Q

Hematoidin can be stained with

A

Gmelins test

Stein iodine test

58
Q

Hemoglobin minus the globin molecule

A

Hematin

59
Q

Black granules formed by malarial parasites

A

Hemozoin

60
Q

Iron free brownish yellow pigment which occurs with Hemosiderin on hemochromatosis

A

Hemofuscin

61
Q

Brown or black pigments normally found in the skin and eyes

A

Melanin

62
Q

Technique use for melanin pigments

A

Masson Fontana technique

63
Q

Give examples of exogenous pigments

A 
Tattoo pigments 
Asbestos
Carbon 
Silica
Iron
Silver
64
Q

Enumerate the 3 artifacts pigments

A

Formaldehyde deposit
Mercuric chloride deposit
Osmium tetroxide deposits

65
Q

Is particularly recommended for staining skeletal system in embryos and fetuses

A

Calcium dye lake reaction

66
Q

2 stain used for bacteria

A

Acid fast stain

Gram stain

67
Q

2stain use in spirochete

A

Rapid giemsa

Levaditi method

68
Q

Fungi stain

A 
Hematoxylin 
Gram staining 
Giemsa stain
Ziehl Neelsen's Method
Metachromasia of capsule
69
Q

4 Special stains use for microorganism

A

Hitchkiss - McManus PAS technique
Bauer feulgen technique
Alicante blue method
Gridley stain for fungi

70
Q

Give the 4 special stain use in Connective tissue

A

Collagen
Elastic fibers
Reticulum connective tissue fiber stains

Histopathology (8 decks)

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