Staining Flashcards
chemical staining
colouration without the use of pigments
enzyme histochemistry
Preserve the section of the tissue without disturbing enzyme activity
-localizing a cell/section
in situ hybridization
used to localize a sequence of DNA and RNA in a biological sample
immunohistochemistry
anti-bodies as probes
direct–> conjugate the marker to the antibody that recognizes the target of interest
indirect–> incubate specific antibody that bind to the antigen in the section
TEM staining
heavy metal staining
confocal microscopy
virtual sectioning of a thick piece of tissue or specimen
basic stains, stain what components?
nucleus, RER in secretory cells
acid stains, stain what components?
normal cytoplasm
what does hematoxylin stain?
- basophilic structures (cell nucleus;containing DNA and nucleoprotein)
- acidophilic structures (organelles containing RNA; ribosomes and rough ER)
what does eosin stain?
cytoplasm (muscle fibres), cell wall, extracellular fibres (
collagen)
periodic acid-schiff reaction
carbs react with periodic acid to produce aldehydes –> convert colourless schiff reagent
metachromatic staining
mast cells stain purple in a blue background
- wright-giemsa stain, toluidinde blue stain
pararosaniline-toludine blue
stains chromatin shades of purple and cytoplasm and collagen a lighter violet
wright-giemsa stain for blood smears
stains white blood cells blue granules basopophilic or neutrophilic
silver impregnation
used to trace nerves, stain golgi, reticular fibres
