Staining Flashcards
chemical staining
colouration without the use of pigments
enzyme histochemistry
Preserve the section of the tissue without disturbing enzyme activity
-localizing a cell/section
in situ hybridization
used to localize a sequence of DNA and RNA in a biological sample
immunohistochemistry
anti-bodies as probes
direct–> conjugate the marker to the antibody that recognizes the target of interest
indirect–> incubate specific antibody that bind to the antigen in the section
TEM staining
heavy metal staining
confocal microscopy
virtual sectioning of a thick piece of tissue or specimen
basic stains, stain what components?
nucleus, RER in secretory cells
acid stains, stain what components?
normal cytoplasm
what does hematoxylin stain?
- basophilic structures (cell nucleus;containing DNA and nucleoprotein)
- acidophilic structures (organelles containing RNA; ribosomes and rough ER)
what does eosin stain?
cytoplasm (muscle fibres), cell wall, extracellular fibres (
collagen)
periodic acid-schiff reaction
carbs react with periodic acid to produce aldehydes –> convert colourless schiff reagent
metachromatic staining
mast cells stain purple in a blue background
- wright-giemsa stain, toluidinde blue stain
pararosaniline-toludine blue
stains chromatin shades of purple and cytoplasm and collagen a lighter violet
wright-giemsa stain for blood smears
stains white blood cells blue granules basopophilic or neutrophilic
silver impregnation
used to trace nerves, stain golgi, reticular fibres
why do we use cryosections for viewing tissue?
paraffin processing will extract lipids
microscopic autoradiography
radiolabeled molecule is injected into animal
- reveals location of newl synthesized gylcoproteins containing fucose
laser scanning microscope
Laser light reflected onto the specimen through the objective lens and if you follow the magenta line of the focal plane→ fluorescence detected is a thick section
