Stain Theory (Wk 6)

Question 1

This is the property of a molecule when double bonds can not be represented by a single diagram.

Answer 1

RESONANCE

Question 2

When a tissue element takes on a different colour from the dye that stains it.

Answer 2

METACHROMASIA

Question 3

An atomic arrangement that allows a molecule to be coloured

Answer 3

CHROMOPHORE

Question 4

This method binds and reduces silver to a visible deposit.

Answer 4

ARGENTAFFIN

Question 5

This is a staining method which involves overstaining and then selectively removing dye from certain areas.

Answer 5

REGRESSIVE

Question 6

This kind of dye has a positive charge.

Answer 6

CATIONIC

Question 7

An ionizable group which allows a dye to be attracted to a tissue component

Answer 7

AUXOCHROME

Question 8

This method requires an external reducing agent to produce a visible deposit of silver.

Answer 8

ARGYROPHIL

Question 9

This type of dye has a negative charge.

Answer 9

ANIONIC

Question 10

This process involves selectively removing stain from unwanted areas until the correct elements are stained.

Answer 10

DIFFERENTIATION

Question 11

An anionic tissue component which when stained with a planar dye and in the presence of water will stain metachromatically

Answer 11

CHROMOTROPE

Question 12

This staining method involves staining until only the correct elements are demonstrated and then stopping.

Answer 12

PROGRESSIVE

Question 13

This dye is colourless until it reacts with the appropriate tissue component.

Answer 13

LEUCO

Question 14

A molecule bearing an chromophoric group

Answer 14

CHROMOGEN

Question 15

This is a metal which is used to link a negatively charged dye to a negatively charged tissue element.

Answer 15

MORDANT

Question 16

This is the term that is applied to colour when key wavelengths are removed from white light.

Answer 16

COMPLIMENTARY COLOUR

Question 17

This dye is the combination of an anionic and a cationic dye.

Answer 17

NEUTRAL

Question 18

A coloured molecule which is used to demonstrate lipids.

Answer 18

LYSOCHROME

Question 19

This type of dye molecule can be either positively or negatively charged.

Answer 19

AMPHOTERIC

Question 20

When a tissue element takes on the same colour as the dye used to colour it.

Answer 20

ORTHOCHROMASIA

Question 21

Substances which have the ability to rotate the plane of polarized light are known as _______

Answer 21

birefringent

Question 22

Unique number used to identify dyes which are listed in Conn’s Biological Stains is known as?

Answer 22

Colour index number

Question 23

Where would you find the colour index number?

Answer 23

Conn’s Biological Stains

Question 24

3 types of weak forces

Answer 24

van der Waals
Hydrophobic
Hydrogen

Question 25

Most common force that attaches dyes to tissue?

Answer 25

Covalent

Question 26

Example of a neutral dye and its components

Answer 26

Romanowsky-Giemsa

Eosin (anionic) + Azure B (cationic)

Question 27

3 chromophores

Answer 27

• Nitro group (NO2)
• Diazo group (N=N)
• Quinoid group

Question 28

In a coordinate covalent bond, ____ atom(s) contributes electrons

Answer 28

only ONE

Question 29

Stain that relies on hydrogen bonding

Answer 29

Congo Red

Question 30

Stain that relies on van der Waals forces

Answer 30

Verhoeff’s

Question 31

Stain that relies on hydrophobic bonding

Answer 31

Oil Red O

Question 32

Stain that relies on covalent bonds

Answer 32

PAS

Question 33

Stain that relies on coordinate covalent bonds

Answer 33

Hematoxylin

Question 34

Staining with lysochromes requires the lipid to be _________ at room temperature

Answer 34

liquid