Microtomy (Wk 2)

Question 1

Cryostat temperature

Answer 1

-20 degC

Question 2

Specialized media required for cryostat which freezes tissue to the right consistency

Answer 2

OCT

Question 3

Muscle biopsies require special procedures known as..?

Answer 3

Quenching

Question 4

Routine freezing with muscle tissue will lead to this artifact

Answer 4

Ice crystal artifact

Question 5

How is tissue quenching achieved

Answer 5

Place muscle in partially frozen isopentane in liquid nitrogen

Question 6

Ultramicrotomes require tissue to be embedded in..?

Answer 6

Plastic

Question 7

Outcome if knife angle <5deg?

Answer 7

Sections thick and thin

Question 8

Outcome if knife angle >5deg?

Answer 8

Sections are scraped rather than cut

Question 9

Most common type of mounting media and its 2 advantages?

Answer 9

Resinous - better resolution and hardens

Question 10

Terminology for 3+ sections WITH trimming between each section

Answer 10

Deepers

Question 11

Terminology for 3+ sections WITHOUT trimming between each section

Answer 11

serial sections