Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Histology > Pigments (Wk 10) > Flashcards

Pigments (Wk 10) Flashcards

1
Q

___________ forms a dark green chelate in the presence of copper

A

Rubeanic acid

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Appearance of asbestos in lung tissue?

A

Brown, beaded dumbbell appearance

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

What is a nevus

A

Benign lesion

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Von Kossa’s - how are visible deposits formed?

A
  • Treat tissues w/ silver nitrate

- Light source reduces silver to visible deposit

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

2 artifact pigments

A

Formalin

Mercury

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Colour and location of bile

A

Yellow-brown

Liver

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Where is chromaffin pigment normally found?

A

Adrenal medulla

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Demonstrated by Turnbull’s Blue stain

A

Ferrous iron

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Melanin immunohistochemical marker

A

S100

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

2 methods that demonstrate calcium

A 
Von Kossa (indirect)
Alizarin Red (direct, but also stains Mg)
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

3 categories of pigments

A
  • Endogenous
  • Artifact
  • Exogenous + minerals
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Can be removed w/ iodine and then sodium thiosulphate

A

Mercury artifact

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

3 non-hematogenous pigments

A

Melanin
Lipofuscin
Chromaffin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

What coats asbestos

A

Proteins + hemosiderin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Colour and location of hemosiderin

A

Yellow-brown

Intracellular (macrophages), sites of hemorrhage

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Substances which occur in living matter and absorb visible light

A

Pigments

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

This amino acid is acted on by an enzyme to be converted to melanin

A

Tyrosine

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

How is melanosis coli demonstrated

A

Methods that demonstrate lipofuscin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

What reduces ammoniacal silver in Masson-Fontana method?

A

Melanin

20
Q

Typical locations of lipofuscin

A

Liver
Heart
Brain

21
Q

2 possible sources of INTERFERENCE causing weak bonds in Congo Red

A
  • Salt

- Alkalinity

22
Q

How is hemoglobin demonstrated?

A

Diamino benzidine + H2O2
OR
Leuco patent blue

23
Q

Main difference in the components of Perl’s vs Turnbull’s stain?

A

Perl’s - ferrOcyanide

Turnbull’s - ferrIcyanide

24
Q

Brown pigment sometimes in macrophages in colon (misc. pigment)

A

Melanosis coli

25
Q

Endogenous pigments can be further subdivided into these 3 categories

A

Hematogenous
Non-hematogenous
Endogenous minerals

26
Q

_________ forms a red product w/ copper

A

Rhodanine

27
Q

Amyloid tissue must be cut at this thickness

A

6-10 microns

28
Q

3 exogenous pigments/minerals

A

Tattoo pigment
Carbon
Asbestos

29
Q

Why isn’t formalin required in MF stain?

A

argentaffin method = no external reducing agent required

30
Q

Stain that demonstrates hemosiderin

A

Perl’s Prussian Blue

31
Q

Tyrosine -> _______ -> melanmin

A

DOPA

32
Q

2 components of Fouchet’s

A

Trichloroacetic acid

Ferric chloride

33
Q

2 other argentaffin substances (not melanin) that may also stain in the MF method

A

Chromaffin

Lipofuscin

34
Q

Weak amyloid staining may be due to these 3 factors

A
  • Prolonged formalin fixation
  • Old control sections
  • Old reagents
35
Q

What do calcium deposits in tissue represent?

A

Previous inflammation or injury

36
Q

3 endogenous mineral pigments

A

Calcium
Copper
Urates

37
Q

Stain that demonstrates bile and how

A

Fouchet’s

Oxidizes bilirubin to green biliverdin

38
Q

Method for demonstrating melanin that involves ferric ions

A

Schmorl’s

39
Q

How to demonstrate asbestos?

A

Perl’s stain of asbestos - shows hemosiderin coating the asbestos

40
Q

2 components of Turnbull’s blue

A

Acid

Potassium ferricyanide

41
Q

2 methods that demonstrate copper

A 
Rubeanic acid (green)
Rhodanine (red)
42
Q

Which stain is this?

Melanin reduces ferric to ferrous ions and then combines with ferricyanide

A

Schmorl’s

43
Q

AKA “wear and tear” pigment

A

Lipofuscin (results from breakdown of lipids)

44
Q

Masson-Fontana counterstain

A

Neutral red

45
Q

2 components of working iron reagent in Perl’s Prussian blue

A

1:1 ratio HCl + potassium ferrOcyanide

Histology (18 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions