Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Histology > Connective Tissues - Trichrome, Reticulin, Elastin (Wk 8) > Flashcards

Connective Tissues - Trichrome, Reticulin, Elastin (Wk 8) Flashcards

1
Q

What colour does COLLAGEN stain in Van Gieson’s

A

Red

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Removes red staining from collagen

A

Phosphomolybdic acid

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

What colour does MUSCLE stain in Masson’s Trichrome

A

Red

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Main coloured staining component in Verhoeff’s

A

hematoxylin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Melanin staining is an ___________ reaction (type of silver rxn)

A

argentaffin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

3 highly cellular tissues that reticulin would be found in

A

Liver
Lymph nodes
Spleen

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

In trichrome, aniline blue would most likely stain what?

A

Collagen

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Type of hematoxylin required in the trichrome method

A

Iron

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Typical stain for unsaturated lipids?

A

Oil Red O

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Reticulin staining is an _________ reaction (type of silver rxn)

A

argyrophil

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Stroma consists of ______ and connective tissue fibers

A

ECM

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Why are only unsaturated lipids stainable w/ Oil Red O

A

Stain requires lipids to be liquid at room temp - only UNsaturated lipids can be liquid at RT (saturated lipids are solid)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Reagent used as a final differentiating wash in trichrome methods

A

Acetic acid

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Chromophore in Oil Red O?

A

Diazo bonds

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

What 2 reagents do you combine to make ammoniacal silver?

A

Silver nitrate + ammonium hydroxide

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

This component has the larger pore size in the conventional theory of trichrome staining?

A

Collagen

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

This is the smaller dye used in Van Gieson’s.

A

Picric acid

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

This general type of hematoxylin is used when doing Van Gieson’s or Masson’s trichromes due to their acidic nature.

A

Iron mordanted

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

Final differentiating solution in many trichrome methods

A

acetic acid

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

Reticulin method - what reagent is used to detect the aldehydes created in the reticulin?

A

Ammoniacal silver

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

Stain that demonstrates elastin

A

Verhoeff’s

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

3 main components of Verhoeff’s

A

Hematoxylin
Ferric chloride
Iodine

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

This elastin stain is derived from lichens

A

Orcein

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
Q

What colour does COLLAGEN stain in Masson’s trichrome?

A

Green or Blue

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
25
Q

Why are the coloured molecules used in lipid stains technically not dyes?

A

No auxochromes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
26
Q

Cause of gray background in Verhoeff’s?

A

Due to iodine which is later removed with alcohol

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
27
Q

Which component in Van Gieson’s is considered to be the cytoplasmic, muscle stain?

A

Picric acid

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
28
Q

Routine stain that collagen stains with?

A

Eosin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
29
Q

Which component in Van Gieson’s will demonstrate collagen?

A

Acid Fuschin

30
Q

What component of Gomori’s Reticulin stain removes unreduced silver and gold?

A

Sodium thiosulphate

31
Q

4 components of Movat’s Pentachrome and what they demonstrate

A

Alcian blue - acid mucoproteins (light blue)
Hematoxylin - nuclei, elastin (dark blue)
Saffron - collagen (yellow)
Acid fuchsin - muscle, fibrin (red)

32
Q

This chemical group is blocked by prolonged fixation in formalin and may result in poor trichrome staining.

A

Amino groups

33
Q

Charge on all tissue components in trichrome method is ___________

A

cationic

34
Q

Supportive structures of organs are called?

A

Stroma

35
Q

Why is IRON hematoxylin required to stain nuclei in trichrome stains?

A

Due to strong acidity of staining solutions (can’t use reg hematoxylin)

36
Q

What is used to differentiate in Verhoeff’s? What is the term for it?

A

Ferric chloride

Mordant differentiation

37
Q

What colour does MUSCLE stain in Van Gieson’s

A

Yellow

38
Q

Elastin is produced by __________ and ______________

A

fibroblasts, smooth muscle cells

39
Q

2 functions of ferric chloride in Verhoeff’s

A

Oxidizing agent

Mordant

40
Q

Oxidizes vicinal diols to produce aldehydes in the reticulin stain

A

Potassium permanganate (could also use periodic acid)

41
Q

This is typically used as a secondary fixative when doing Masson’s Trichrome

A

Bouin’s

42
Q

Stain that involves “mordant differentiation”

A

Verhoeff’s

43
Q

Why is Bouin’s routinely used as a secondary fixative before trichrome staining?

A

Restores the lost positive charges from formalin fixation

44
Q

Intermediate reagent used in some trichrome methods (mordant/colourless intermediate dye)

A

phosphomolybdic acid

45
Q

Stain for saturated lipids?

A

Sudan Black

46
Q

What component in the reticulin stain reduces ammoniacal silver to metallic silver?

A

Formalin

47
Q

What is the charge of the dyes used in most trichrome methods?

A

Anionic

48
Q

Ratio of picric/acid fuchsin in Van Gieson’s

A

50:1

49
Q

3 types of connective tissue fibers

A
  • Reticulin
  • Elastin
  • Collagen
50
Q

Usual counterstain in Verhoeff’s?

A

Van Gieson’s

51
Q

2 different words that refer to the coloured molecules in lipid stains

A

Lysochromes

Lipochromes

52
Q

What is the general name for the stains used to differentiate between collagen and muscle.

A

Trichrome

53
Q

This stain is a simpler and older trichrome method

A

Van Gieson’s

54
Q

In the Gomori’s Reticulin silver method, what is used to detect aldehydes in the reticulin fibers?

A

ammoniacal silver

55
Q

What is the process called in Gomori’s Reticulin method which uses gold chloride?

A

Toning

56
Q

What stains collagen yellow in Movat’s pentachrome?

A

Saffron (not picric acid!)

57
Q

2 most common special stains to demonstrate collagen

A

Masson’s trichrome stain

Van Gieson’s stain

58
Q

2 examples of stroma?

A

Collagen, reticulin

59
Q

Precaution when performing Verhoeff’s

A

Possible to over-differentiate

60
Q

How to properly dispose of silver reagents?

A

Neutralize with salt (otherwise can form explosive compounds)

61
Q

This reagent removes the connective tissue dye in Masson’s Trichrome.

A

Acetic Acid

62
Q

Why must you avoid prolonged washing in water or dilute alcohol when doing Van Gieson’s?

A

Picric acid is water-soluble

63
Q

What is the sensitizing reagent in reticulin stain?

A

Iron alum (follows oxidizing agent to enhance contrast)

64
Q

What does it mean when the stain is an argentaffin reaction?

A

External reducing agent is NOT required to produce a visible product

65
Q

This type of connective tissue fiber would be found in highly cellular tissues such as liver, lymph nodes, spleen

A

Reticulin

66
Q

Main functional cells of an organ are called?

A

Parenchyma

e.g. hepatocytes

67
Q

This term applies to items which are capable of reducing and binding silver, but require an external reducing agent in order to be visible.

A

Argyrophil

68
Q

This is another name for Reticulin.

A

Type III Collagen

69
Q

Purpose of potassium metabisulfite in reticulin stain?

A

Removes brown discolouration from potassium permanganate (oxidizing agent)

70
Q

Why is using charged slides suggested for silver staining?

A

Ammoniacal silver is very alkaline which may cause the tissue to lift off the slide

Histology (18 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions