Spirochetes Flashcards
Genus under Spirochetes
- Treponema
- Borrelia
- Leptospira
Spirochetes are ____ organisms
a. Gram positive
b. Gram negative
c. Acid fast
b. Gram negative
Motility of spirochetes
Corkscrew motility
Microscope used to visualize Treponema and Borrelia
a. Darkfield Microscope
b. Phase contrast microscope
c. Brightfield microscope
d. All of the above
e. None of the above
e. None of the above
Borrelia is the only genus under spirochetes that can be visualized using Bright field microscopy
Leptospira and Treponema are visualized using Dark field microscopy or Phase contrast microscopy
Stain used to identify Spirochetes
Silver stain
Spirochetes that multiplies by binary fission
- Borrelia
- Leptospira
Spirochetes that multiplies by transverse fission
Treponema
Blood Spirochetes
Borrelia
Tightly coiled with one (1) or both ends bent to form a hook
Leptospira
Describe appearance of Treponema spp. coils.
- Consist of 4-14 coils per organism
- Regularly spaced
This spirochetes is arthropod-borne and acquired through bites.
Borrelia
Describe appearance of Borrelia spp. coils.
Loosely coiled
Culture media used for cultivating Borrelia spp.
Kelly medium
Culture media used for cultivating Leptospira spp.
Fletcher’s medium
How is the viability of T. pallidum maintained?
Viability is maintained using testicular chancre or rabbits
T. pallidum is non-culturable in vitro.
a. True
b. False
a. True
Agent of syphilis
T. pallidum
Other terms for syphilis
- Great pox
- French disease
How is syphilis transmitted?
a. Sexually
b. Blood transfusion
c. Vertical transmission from mother to child
d. All of the above
e. None of the above
d. All of the above
How many stages does syphilis have?
Four (4) stages
Syphilis stage where there is absence of lesions or symptoms
Latent stage
Syphilis stage where the patient develops lesion in the genitalia
Primary stage
Syphilis stage where the patient develops extragenital lesions
Secondary stage
Syphilis stage where the patient develops lesion in deep organs of the body
Tertiary stage
This is the primary syphilitic lesion
Hard chancre / Hunterian chancre
This is the secondary syphilitic lesion
Condyloma latum
What happens during the latent stage of syphilis?
Absence of lesions and symptoms but positive in blood test
This is the tertiary syphilitic lesion
Gumma’s
Agent of STD
Treponema cuniculi
T. cuniculi is known for?
Venereal spirochetosis
This Treponema spp. causes Trench mouth
T. vincentii
Other term for Trench mouth
Vincent’s stomatitis
What are the three (3) significant Treponema pallidum subspecies that cause non-venereal syphilis?
- T. pallidum spp. pertenue
- T. pallidum spp. carateum
- T. pallidum spp. endemicum
Match:
- T. pallidum spp. carateum
- T. pallidum spp. pertenue
- T. pallidum spp. endemicum
a. Yaws
b. Pinta
c. Bejel
1-B
2-A
3-C
What is the mode of transmission of the three subspecies of T. pallidum?
Direct contact with lesions
Disease caused by the subspecies of T. pallidum is characterized by?
Presence of lesions in the body
Other term for Bejel disease
Endemicum syphilis
How is Borrelia detected?
- Sero test
- Examination of Giemsa stained smears
Borrelia spp. that causes Lyme Disease
Borrelia burgdorferi
Vector of Borrelia burgdorferri
Ixodes ticks aka Deer ticks or Black Legged ticks
This disease is described as tick-borne relapsing fever
Lyme Disease
How many stages are there in Lyme Disease?
Three stages
What happens during the third stage of Lyme Disease?
Chronic arthritis and development neurologic abnormalities/symptoms
What happens during the second stage of Lyme Disease?
Dissemination of ECM to other parts of the body
What happens during the first stage of Lyme Disease?
Develops bull’s eye rash in the bite site
Bull’s eye rash is also known as
Erythema Chronicum Migrans (ECM)
Causative agent of louse-borne relapsing fever
B. recurrentis
Vector(s) of B. recurrentis
Pediculus spp.
a. P. humanus capitis
b. P. humanus corporis
Pediculus spp. known as head louse
P. humanus capitis
Pediculus spp. known as body louse
P. humanus corporis
Other agents of tick-borne relapsing fever that is transmitted by Ornithodoros ticks (soft ticks)
- Borrelia duttonii
- Borrelia parkeri
- Borrelia hermsii
Media used for Leptospira
Fletcher’s media
Hallmark of immune phase of Leptospirosis
Aseptic meningitis
Serologic gold standard for Leptospirosis detection
Microscopic agglutination test
What are the three tests that can be performed to detect Leptospirosis?
- Serologic Test
- Microscopy
- Culture
Causative agent of Leptospirosis (human and animal)
Leptospira interrogans
What are the two (2) phases of Leptospirosis?
- Anicteric phase
- Icteric phase
This phase of leptospirosis is characterized by septicemia (high fever and headache)
Anicteric phase
This phase of leptospirosis is characterized by involvement of liver and kidney
Icteric phase
Specimen of choice for the 1st week of Leptospirosis infection
- Blood
- CSF
Specimen of choice for the 2nd week of Leptospirosis infection
Urine