Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Bacte ulit kinangina > Anaerobes > Flashcards

Anaerobes Flashcards

1
Q

Organisms that does not require oxygen for growth

A

Anaerobes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

What are the four (4) macroscopic exam done to identify anaerobes?

A
  1. Presence of foul odor
  2. Presence of blackening/black color of specimen
  3. Fluorescence
  4. Presence of sulfur granules
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Three (3) anaerobes that emit foul odor

A
  1. Clostridium difficile
  2. Fusobacterium
  3. Porphyromonas
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Equipment used to provide an anaerobic environment

A

GASPAK Jar

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Indicators of GASPAK Jar

A

Methylene Blue or Resazurin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Component of GASPAK jar that ensures oxygen free environment

A

Palladium Catalyst

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

What happens when there’s a presence of oxygen in GASPAK jar?

A

The jar turns red or blue depending on the indicator that is present.

Methyl Blue (Blue)
Resazurin (Pink)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Absence of color in the GASPAK jar indicates what?

A

Absence of air

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Common failure of GASPAK jar

A

Inactivation of catalyst due to repeated use

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Gram negative anaerobic bacilli that produce black pigment and brick red fluorescence under UV

A

Porphyromonas and Prevotella

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Anaerobes that produces red fluorescence that rapidly fades

A

Veilonella

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

The color of fluorescence Fusobacterium nucleatum emits

A

Chartreuse fluorescence (Yellow)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Three (3) anaerobes with sulfur granules

A
  1. Actinomyces,
  2. Propionibacterium/Cutibacterium acnes
  3. Eubacterium nodatum
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Anaerobic organism that is microscopically with tapered ends appearing like toothpick

A

Fusobacterium nucleatum

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Anaerobic organism with “bifid” ends/appearing like bone-shaped dog biscuit

A

Bifidobacterium

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Anaerobic bacilli that assumes safety pin appearance

A

Bacteroides fragilis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

Bacteroides fragilis shows resistance to which antibiotics in Potency Disk Test?

A
  1. Kanamycin
  2. Vancomycin
  3. Colistin
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

Bacteroides fragilis is bile tolerant.

a. True
b. False

A

a. True

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

Anaerobic bacilli that causes aspiration pneumonia

A

Bacteroides fragilis

20
Q

Anaerobic diphtheroid

A

Propionibacterium spp.

21
Q

This test is done for rapid detection of Cutibacterium acnes

A

Spot Indole Test

22
Q

What is the positive result for spot indole test?

A

Development of blue/green color

23
Q

What is the negative result for spot indole test?

A

Pink/orange color

24
Q

The reagent used in Spot Indole Test

A

P-dimethylaminocinnamaldehyde

25
Q

Match: Spot Indole Test

A. Cutibacterium acnes
B. Propionibacterium spp.

  1. Negative (-)
  2. Positive (+)

a. Pink/orange
b. Blue/green

A

Cutibacterium acnes - Positive - Blue/Green

Propionibacterium spp. - Negative - Pink/Red

26
Q

The purpose of this test is to verify gram staining result of anaerobic bacteria

A

Potency Disk Test

27
Q

Antibiotics used in Potency Disk Test and their concentration

A
  1. Kanamycin (1000 µg)
  2. Vancomycin (5 µg)
  3. Colistin (10 µg)
28
Q

Identify the bacteria:

Result in Potency Disk Test: RRR

A

Bacteroides fragilis

29
Q

Identify the bacteria:

Result in Potency Disk Test: SRS

A
  1. Fusobacterium
  2. Bacteroides ureolyticus
  3. Veilonella
30
Q

Identify the bacteria:

Result in Potency Disk Test: SSR

A

Clostridium

31
Q

Identify the bacteria:

Result in Potency Disk Test: RSR

A
  1. Porphyromonas
  2. P. anaerobius
32
Q

Identify the bacteria:

Result in Potency Disk Test: RRS

A

Prevotella

33
Q

Anticoagulant used in bacterial culture and its concentration

A

Sodium Polyanethol Sulfonate (0.25%)

34
Q

Disadvantage of SPS

A

Peptostreptococcus anaerobius is sensitive.

35
Q

How do you counteract the inhibitory property of SPS?

A

Add 1% gelatin

36
Q

Spore-forming anaerobes that exhibit swarming on BAP

A
  1. Clostridium tetani
  2. Clostridium septicum
37
Q

Anaerobic organism that develops colonies appearing like molar tooth

A

Actinomyces israelii

38
Q

Actinomyces israelii affects soft tissues and can cause?

A

Actinomycosis of Jaw (Lumpy Jaw)

39
Q

Anaerobic staphylococcus

A

Peptococcus niger

40
Q

Anaerobic streptococcus

A

Peptostreptococcus

41
Q

Anaerobic organism that is an important component of dental biofilm

A

Prevotella

42
Q

Sign of growth in Thioglycolate media

A

Turbidity

43
Q

A versatile media an often used in the lab because it can allow
growth of anaerobe, facultative anaerobes and aerobes

A

Thioglycolate media

44
Q

Sign of growth of anaerobes on Thioglycolate media

A

Turbidity at the bottom of the media

45
Q

Sign of growth of facultative anaerobes on Thioglycolate media

A

Diffused growth all throughout the media

46
Q

Sign of growth of strict aerobes on Thioglycolate media

A

Turbidity towards the surface

Bacte ulit kinangina (9 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions