Non-fermenters

Question 1

These four (4) genus are regarded as non-fermenters and nosocomial pathogens

Answer 1

1. Genus Pseudomonas
2. Genus Stenotrophomonas
3. Genus Burkholderia
4. Genus Acinetobacter

Question 2

Non-fermenters are oxidizers.

a. True
b. False

Answer 2

a. True

Both have the same definition but the term non-fermenter is most commonly used than oxidizers.

Question 3

This is the #1 ICU isolate and the agent of pneumonia in those with cystic fibrosis

Answer 3

Pseudomonas aeruginosa

Question 4

A condition caused by P. aeruginosa that can be acquired from wet environment

Answer 4

Jacuzzi or Hot Tub syndrome

Question 5

Odor(s) that P. aeruginosa colonies produce

Answer 5

1. Overripe grape
2. Taco-corn tortilla like odor

Question 6

Characteristics of P. aeruginosa: (3)

Answer 6

1. Strict aerobes
2. Oxidase (+)
3. B-hemolytic on BAP

Question 7

P. aeruginosa can produce which pigment?

a. Pyoverdin
b. Pyocyanin
c. Pyorubin
d. B&C
e. A&B

Answer 7

E. A&B

P. aeruginosa can produce both pyoverdin and pyocyanin

Question 8

Media used for initial isolation of P. aeruginosa

Answer 8

Cetrimide agar

Question 9

Cetrimide agar is selective media because it is toxic to many bacteria but not to P. fluorescens.

a. True
b. False

Answer 9

b. False

Cetrimide agar is toxic to many bacteria and is used for isolating P. aeruginosa, not P. fluorescens.

Question 10

Genus that is non-fermenter, oxidase (-), and non-motile

Answer 10

Acinetobacter

Question 11

Another genus that is non-fermenter with two (2) significant pathogens.

Answer 11

Burkholderia

Question 12

Two (2) significant pathogen of Burkholderia:

Answer 12

1. B. mallei
2. B. pseudomallei

Question 13

Burkholderia spp. that causes Melioidosis

Answer 13

B. pseudomallei

Question 14

Burkholderia spp. that causes Glander’s disease

Answer 14

B. mallei

Question 15

Match:

Burkholderia spp., their other names, and the disease they cause

1. B. mallei
2. B. pseudomallei

a. Glander’s Bacillus
b. Whitmore’s Bacillus

Answer 15

1. B. mallei — a. Glander’s bacillus
2. B. pseudomallei — b. Whitmore’s bacillus

Question 16

These non-fermenters are both capable of producing wrinkled colonies

Answer 16

1. Pseudomonas stutzeri
2. Burkholderia pseudomallei

Question 17

B. pseudomallei emits what kind of odor in which media?

Answer 17

Earthy odor in ashdown media

Question 18

Agents of transfusion associated septicemia

Answer 18

P. fluorescens and P. putida

Question 19

P. fluorescens and P. putida can produce which pigment?

a. Pyoverdin
b. Pyocyanin
c. AOTA
d. NOTA

Answer 19

a. Pyoverdin

Question 20

Differential test for P. fluorescens and P. putida

Answer 20

Gelatin Hydrolysis Test

Question 21

Match:

Gelatin Hydrolysis Test

1. P. fluorescens
2. P. putida

a. Negative (-)
b. Positive (+)

Answer 21

1. P. fluorescence — b. Positive
2. P. putida — a. Negative