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Molecular Biology > Specimen and nucleic acid storage > Flashcards

Specimen and nucleic acid storage Flashcards

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1
Q

DNA
whole blood, buffy coat, bone marrow
22- 25°

A

24 hrs

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2
Q

DNA
whole blood, buffy coat, bone marrow
2-8°

A

72 hrs

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3
Q

DNA
whole blood, buffy coat, bone marrow
-20°

A

1 year

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4
Q

DNA
whole blood, buffy coat, bone marrow
-70°

A

> 1 yr

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5
Q

DNA
tissue
22- 25°

A

not recommended

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6
Q

DNA
tissue
2-8°

A

24 hrs

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7
Q

DNA
tissue
-20°

A

at least 2 weeks

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8
Q

DNA
tissue
-70°

A

at least 2 years

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9
Q

DNA
culture
22-25°

A

24 hrs

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10
Q

DNA
culture
2-8°

A

72 hrs

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11
Q

DNA
culture
-20°

A

2-4 weeks

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12
Q

DNA
culture
-70°

A

> 1 yr

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13
Q

DNA
cell lysate in guanidine isothiocyanate
22-25°

A

1-2 weeks

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14
Q

RNA
whole blood, bufft coat, bone marrow
22-25°

A

not recommended

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15
Q

RNA
whole blood, bufft coat, bone marrow
2-8°

A

24 hrs

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16
Q

RNA
whole blood, bufft coat, bone marrow
-20°

A

2-4 weeks

17
Q

RNA
whole blood, bufft coat, bone marrow
-70°

A

> 1yr

18
Q

RNA
fluids in specialty
22-25°

A

5 days

19
Q

RNA
RNA protection tubes
2-8°

A

7 days

20
Q

RNA
RNA protection tubes
-20°

A

2-4 weeks

21
Q

RNA
RNA protection tubes
-70°

A

7 months

22
Q

RNA
tissue
20-25, 2-8, -20°

A

not recommended

23
Q

RNA
tissue
-70, -140 °

A

2 yrs

24
Q

RNA
cell lysates in guanidine isothicyanate
22-25°

A

1-2 weeks

25
Q

RNA
cell lysates in RNA storage solution
22-25°

A

1 week

26
Q

RNA
cell lysates in RNA storage solution
2-8°

A

1 month

27
Q

RNA
cell lysates in RNA storage solution
-20°

A

1 yr

28
Q

RNA
cell lysates in RNA storage solution
-70°

A

more than 1 yr

29
Q

DNA
freeze dried or dried on collection paper
22- 25°

A

> 15 yrs

30
Q

DNA
TE buffer or DNase free water
22-25°

A

4 months

31
Q

DNA
TE buffer or DNase free water
2-8°

A

1-3 yrs

32
Q

DNA
TE buffer or DNase free water
-20°

A

7 years

33
Q

DNA
TE buffer or DNase free water
-70°

A

more than 7 years

34
Q

RNA
TE buffer or RNase free water (DEPC)
22-25, 2-8°

A

not recommended

35
Q

RNA
TE buffer or RNase free water (DEPC)
-20, -70°

A

up to 1 month

36
Q

RNA
ethanol
-20, -70°

A

6 months

37
Q

RNA
RNA storage solution
-20°

A

more than 1 month

Molecular Biology (12 decks)

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