Specimen and nucleic acid storage

Question 1

DNA
whole blood, buffy coat, bone marrow
22- 25°

Answer 1

24 hrs

Question 2

DNA
whole blood, buffy coat, bone marrow
2-8°

Answer 2

72 hrs

Question 3

DNA
whole blood, buffy coat, bone marrow
-20°

Answer 3

1 year

Question 4

DNA
whole blood, buffy coat, bone marrow
-70°

Answer 4

> 1 yr

Question 5

DNA
tissue
22- 25°

Answer 5

not recommended

Question 6

DNA
tissue
2-8°

Answer 6

24 hrs

Question 7

DNA
tissue
-20°

Answer 7

at least 2 weeks

Question 8

DNA
tissue
-70°

Answer 8

at least 2 years

Question 9

DNA
culture
22-25°

Answer 9

24 hrs

Question 10

DNA
culture
2-8°

Answer 10

72 hrs

Question 11

DNA
culture
-20°

Answer 11

2-4 weeks

Question 12

DNA
culture
-70°

Answer 12

> 1 yr

Question 13

DNA
cell lysate in guanidine isothiocyanate
22-25°

Answer 13

1-2 weeks

Question 14

RNA
whole blood, bufft coat, bone marrow
22-25°

Answer 14

not recommended

Question 15

RNA
whole blood, bufft coat, bone marrow
2-8°

Answer 15

24 hrs

Question 16

RNA
whole blood, bufft coat, bone marrow
-20°

Answer 16

2-4 weeks

Question 17

RNA
whole blood, bufft coat, bone marrow
-70°

Answer 17

> 1yr

Question 18

RNA
fluids in specialty
22-25°

Answer 18

5 days

Question 19

RNA
RNA protection tubes
2-8°

Answer 19

7 days

Question 20

RNA
RNA protection tubes
-20°

Answer 20

2-4 weeks

Question 21

RNA
RNA protection tubes
-70°

Answer 21

7 months

Question 22

RNA
tissue
20-25, 2-8, -20°

Answer 22

not recommended

Question 23

RNA
tissue
-70, -140 °

Answer 23

2 yrs

Question 24

RNA
cell lysates in guanidine isothicyanate
22-25°

Answer 24

1-2 weeks

Question 25

RNA cell lysates in RNA storage solution 22-25°

Answer 25

1 week

Question 26

RNA cell lysates in RNA storage solution 2-8°

Answer 26

1 month

Question 27

RNA cell lysates in RNA storage solution -20°

Answer 27

1 yr

Question 28

RNA cell lysates in RNA storage solution -70°

Answer 28

more than 1 yr

Question 29

DNA freeze dried or dried on collection paper 22- 25°

Answer 29

>15 yrs

Question 30

DNA TE buffer or DNase free water 22-25°

Answer 30

4 months

Question 31

DNA TE buffer or DNase free water 2-8°

Answer 31

1-3 yrs

Question 32

DNA TE buffer or DNase free water -20°

Answer 32

7 years

Question 33

DNA TE buffer or DNase free water -70°

Answer 33

more than 7 years

Question 34

RNA TE buffer or RNase free water (DEPC) 22-25, 2-8°

Answer 34

not recommended

Question 35

RNA TE buffer or RNase free water (DEPC) -20, -70°

Answer 35

up to 1 month

Question 36

RNA ethanol -20, -70°

Answer 36

6 months

Question 37

RNA RNA storage solution -20°

Answer 37

more than 1 month