Other methods of DNA extraction

Question 1

Used for crud cell lysates where DNA molecules separated based on net molecular charge or molecular size

Answer 1

Chromatography based extraction method

Question 2

EtBr-CsCl centrifugation method

Answer 2

DNA sample is mixed with Cesium Chloride, ultracentrifuged at 10,000 to 12,000 rpm for > 10 hrs

Question 3

DNA molecules separate based on density

Answer 3

EtBr-CsCl centirfugation method

Question 4

acts as intercalating agent which allows supercoiled DNA to accumulate at lower densities

Answer 4

ethidium bromide

Question 5

allows interaction with negatively charged DNA

Answer 5

Cesium chloride

Question 6

used for extracting plasmid DNA

Answer 6

alkaline extraction

Question 7

alkaline extraction

Answer 7

sample suspended in NaOH and SDS (denaturation of chromosomal DNA)
potassium acetate (neutralize, renature, precipitate)
plasmid DNA in the supernatant

Question 8

cellulose based paper

Answer 8

impregnated with buffer, detergent, chelatinga agents, DNA washed from the material

Question 9

styrenedivinylbenzene material which chelates metal ions and act as cofactor for DNases

Answer 9

Chelex resin

Question 10

can be used for tissue samples

Answer 10

Chelex 100- Extraction

Question 11

result of Chelex 100 extraction

Answer 11

ssDNA in the supernatant

Question 12

prevents DNA from veing digested by DNases during boiling

Answer 12

Chelex

Question 13

appropriate for plant cell samplea

Answer 13

Filter paper based DNA extraction method

Question 14

Filter paper based DNA extraction method

Answer 14

96 well spin plate
each well contains disc of Whatman Filter paper
fikter using centrifuge

Question 15

CTAB is useful for

Answer 15

organisms with large polysaccharides
plants
gram negative bacteria

Question 16

complicated, expensive, time consuming and requires large scale bacterial cultures

Answer 16

CsCl-EtBr method