Other methods of DNA extraction Flashcards

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1
Q

Used for crud cell lysates where DNA molecules separated based on net molecular charge or molecular size

A

Chromatography based extraction method

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2
Q

EtBr-CsCl centrifugation method

A

DNA sample is mixed with Cesium Chloride, ultracentrifuged at 10,000 to 12,000 rpm for > 10 hrs

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3
Q

DNA molecules separate based on density

A

EtBr-CsCl centirfugation method

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4
Q

acts as intercalating agent which allows supercoiled DNA to accumulate at lower densities

A

ethidium bromide

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5
Q

allows interaction with negatively charged DNA

A

Cesium chloride

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6
Q

used for extracting plasmid DNA

A

alkaline extraction

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7
Q

alkaline extraction

A

sample suspended in NaOH and SDS (denaturation of chromosomal DNA)
potassium acetate (neutralize, renature, precipitate)
plasmid DNA in the supernatant

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8
Q

cellulose based paper

A

impregnated with buffer, detergent, chelatinga agents, DNA washed from the material

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9
Q

styrenedivinylbenzene material which chelates metal ions and act as cofactor for DNases

A

Chelex resin

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10
Q

can be used for tissue samples

A

Chelex 100- Extraction

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11
Q

result of Chelex 100 extraction

A

ssDNA in the supernatant

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12
Q

prevents DNA from veing digested by DNases during boiling

A

Chelex

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13
Q

appropriate for plant cell samplea

A

Filter paper based DNA extraction method

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14
Q

Filter paper based DNA extraction method

A

96 well spin plate
each well contains disc of Whatman Filter paper
fikter using centrifuge

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15
Q

CTAB is useful for

A

organisms with large polysaccharides
plants
gram negative bacteria

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16
Q

complicated, expensive, time consuming and requires large scale bacterial cultures

A

CsCl-EtBr method