Lab Activity 1: Introduction to Molecular Biology Laboratory

Question 1

principle of physically separate work areas

Answer 1

barrier containment

Question 2

4 work areas in the laboratory

Answer 2

reagent preparation
sample preparation
amplification
post amplification

Question 3

air pressure in clean rooms

Answer 3

positive air pressure
to prevent entry of airborne contaminant

Question 4

what are clean rooms

Answer 4

sample preparation
reagent preparation

Question 5

dirty rooms have ______ air pressure

Answer 5

negative
to impede anything from coming out

Question 6

inexpensive way of creating differential pressure in the lab

Answer 6

anteroom

Question 7

True or false: The door to the inside and to the outside of the anteroom can be opened at the same time

Answer 7

False

Question 8

containment principles, technologies and practices that are implemented to prevent unintentional exposure to or accidental release of biological mayerial

Answer 8

biosafety

Question 9

protection, control and accountability for biological agents and toxins to prevent their loss, theft, misuse, diversion, unauthorized access or intentional unauthorized release

Answer 9

biosecurity

Question 10

oncludes all utensils, containers, tissue paper, that has been in contact with hazardous material

Answer 10

biohazard waste (yellow)

Question 11

hypodermic syringe needles, scalpel blase, glass capillary pipettes, microscope slides

Answer 11

sharps (yellow bench top)

Question 12

procedures important to minimize contamination from instruments, personnel and patient

Answer 12

aseptic techniques