SPECIAL STAINS Flashcards

Part 1

1
Q

What is the most used satin in the lab

A

H & E

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2
Q

mention what haematoxylin and eosin stains

A

Haematoxylin stain cell nuclei to blue while eosin stains stains other structures to pink or red

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3
Q

Why do we need an extra help of special stains in the lab or research

A

In order to reach a diagnosis or evaluate a tissue to differentiate components that have already been seen in H & E

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3
Q

Why is H & E mostly used

A

It highlight the details in tissues and cells

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3
Q

What questions does H & E raises

A
  1. what type of tissue is present
  2. which tissue component is that
  3. Is that an organism
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3
Q

Why is the positive control needed during special staining

A
  1. positive control helps to determine whether the special stain is working
  2. Served as a reference of what the substance should look like in test tissue section
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4
Q

Why is H & E still being used

A

Is because this stain is important for providing detail of tissue and cell structure as well as differentiation

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5
Q

Why are special stains used

A

Is because H & E can not provide all the information needed by the pathologist

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6
Q

why are special stains called so

A

Its because they are not routinely used

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