Lecture 3 Flashcards
What is abbrevition of DPX
Distyrene plasticizer xylene
What is the abreviation XAN
Xanthomas
What two stains are used to stain the cytoplasm in papanicolau stain
- Eosin Y
- Light green
Mention all the 3 dyes of papanicolou stain
- Orange G
- Eosin Y
- Light green
mention the stains for Eosin Azure
- Light green
- Eosin y
what stain stains the nucleus for nucleus detail
Hematoxylin
What two names are given to a cell when it is stained blue or blue green in a pap smear staining
- Basophilia
- Cyanophilia
what name is given to a cell when it stains blue in a pap smear staining
acidophilia
Eosinophilia
What name is given to a cell when it stains orange in pap smeae staining
Orangeophilia
what conclusion is given when a cell stains grey-blue
indeterminate
what is cytoplasmic cyanophilia associated with
metabolically active cells
staining ribonucleic acid
what is cytoplasmic acidophilia associated with
- abundance of nonribosomal organelles e.g mitochondria
what is Cytoplasmic orangeophilia associated with
keratinization
what parts of a cytoplasm will show a blue-green colour
ribonucleic acid
ribosomes
in the cytoplasm what components will show a pink colour
basic proteins in the cytoplasm
which cells contains keratin
squamous cell
What is the significance of cytoplasmic acidophilia in a cell
Cytoplasmic acidophilia is associated with an abundance of nonribosomal organelles, such as mitochondria.
What colors can cells be stained using the Papanicolaou stain?
Cells can be stained blue or blue-green (basophilia, cyanophilia), pink (acidophilia, eosinophilia), orange (orangeophilia), or indeterminate (gray-blue)
What do Orange G and eosin Y stain?
Orange G and eosin Y are acid dyes that stain basic proteins, such as prekeratin
What is cytoplasmic orangeophilia associated with?
Cytoplasmic orangeophilia is associated with keratinization.
Can cellular degeneration affect staining?
Yes, cellular degeneration can result in intense eosinophilia or orangeophilia, even in nonsquamous-derived cells.
What does light green specifically bind to
Light green has an affinity for ribonucleic acids (RNA) in ribosomes
