SPECIAL: CITRIC ACID CYCLE

Question 1

Complete process of Citric Acid Cycle (By step)

Answer 1

Step 1: Transfer of Acetyl-CoA
Step 2: Isomerization
Step 3: Oxidation/Decarboxylation
Step 4: Rejoining with CoA (further oxidation)
Step 5: Formation of GTP
Step 6: Oxidation of FAD
Step 7: Hydration
Step 8: Final Oxidation

Question 2

How is Acetyl-CoA produced?

Answer 2

-Pyruvate enters the mitochondrial matrix
-The Pyruvate bind with Coenzyme A
-Then undergoes decarboxylation and oxidation in the presence of NAD+ (converted to NADH)
-Acetyl-CoA is formed

Question 3

What is done before Citric Acid Cycle?

Answer 3

Formation of Acetyl-CoA

Question 4

Citric Acid Cycle: What is the reactant and product in 1st Step?

Answer 4

Reactant: Acetyl-CoA + Oxaloacetate
Product: Citrate

Question 5

Citric Acid Cycle: What is the reactant and product in 4th Step?

Answer 5

Reactant: a (alpha)-ketoglutarate
Product: Succinyl- CoA

Question 5

Citric Acid Cycle: What is the reactant and product in 2nd Step?

Answer 5

Reactant: Citrate
Product: Isocitrate

Question 6

Citric Acid Cycle: What is the reactant and product in 3rd Step?

Answer 6

Reactant: Isocitrate
Product: a (alpha)- ketoglutarate

Question 7

Citric Acid Cycle: What is the reactant and product in 5th Step?

Answer 7

Reactant: Succinyl-CoA
Product: Succinate

Question 8

Citric Acid Cycle: What is the reactant and product in 6th Step?

Answer 8

Reactant: Succinate
Product: Fumarate

Question 8

Citric Acid Cycle: What is the reactant and product in 7th Step?

Answer 8

Reactant: Fumarate
Product: Malate

Question 9

Citric Acid Cycle: What is the reactant and product in 8th Step?

Answer 9

Reactant: Malate
Product: Oxaloacetate

Question 10

Citric Acid Cycle: What is the enzyme used in 1st Step?

Answer 10

Citrate Synthase

Question 11

Citric Acid Cycle: What is the enzyme used in 2nd Step?

Answer 11

Aconitase

Question 12

Citric Acid Cycle: What is the enzyme used in 3rd Step?

Answer 12

Isocitrate dehydrogenase

Question 13

Citric Acid Cycle: What is the enzyme used in 4th Step?

Answer 13

a(alpha)-ketoglutarate dehydrogenase

Question 13

Citric Acid Cycle: What is the enzyme used in 5th Step?

Answer 13

succinyl-CoA synthetase

Question 14

Citric Acid Cycle: What is the enzyme used in 6th Step?

Answer 14

Succinate dehydrogenases

Question 15

Citric Acid Cycle: What is the enzyme used in 7th Step?

Answer 15

Fumarase

Question 16

Citric Acid Cycle: What is the enzyme used in 8th Step?

Answer 16

Malate dehydrogenase

Question 17

Citric Acid Cycle: Details to note in Step 2

Answer 17

-1 water loss
-1 water gained

Question 18

Citric Acid Cycle: Details to note in Step 3

Answer 18

-Used 1 NAD+
-Produced NADH + CO2

Question 19

Citric Acid Cycle: Details to note in Step 4

Answer 19

-Uses 1 NAD+
-Produced NADH + CO2

Question 20

Citric Acid Cycle: Details to note in Step 5

Answer 20

-Uses 1 Phosphate
-Produces GTP (Guanosine Triphosphate)

Question 21

Citric Acid Cycle: Details to note in Step 6

Answer 21

-Uses 1 FAD
-Produces FADH2

Question 22

Citric Acid Cycle: Details to note in Step 7

Answer 22

1 water molecule gained

Question 23

Citric Acid Cycle: Details to note in Step 8

Answer 23

-Uses 1 NAD+
-Produces NADH

Question 24

Net Product of Citric Acid Cycle

Answer 24

6 NADH + 2 FADH2 + 2 ATP per glucose molecule