Small animal breeding Flashcards

1
Q

Where do we deposit semen in dog AI

A

In the fornix

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2
Q

What route do we take into the vagina into the dog

A

In a small bit then directly up then continue forwards
NB: be aware of the clitoral fossa; can get pipette stuck there and mistakenly inseminate there

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3
Q

Bitch repro tract basics

A

2 ovaries; 2 uterine horns; 1 uterine body; cervix, vagina, vestibule

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4
Q

Why is it normal to see cavitation on some CLs

A

Because multiple follicles ovulating over course of a few hours; will see CLs at various stages of the luteal phase
- Have fluid in them early one

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5
Q

Two important features in dog sexual anatomy

A

Os penis = bone in penis; keeps rigid even when not erect; must be careful not to damage

Bulbus glandis = vascularised structure that wraps around base of penis; creates ‘copulatory lock’ to stop semen getting out

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6
Q

What can we use to tell if a cat has been castrated/ still has active androgen production

A

Look for spikes on penis; need activate maintenance do disappear within weeks without

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7
Q

What is different about male cat anatomy vs dog

A

Penis faces backwards
Spikes on penis for stimulation to induce ovulation

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8
Q

What age do female dogs reach puberty

A

Small dogs: 6-8 months
Large dogs: 12 months
Giant breeds (+ greyhounds, wolf hounds): 18-20 months

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9
Q

When do spermatozoa first appear in dog ejaculate

A

7-9 months

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10
Q

What age do male dogs reach puberty

A

Small: 6 months
Large: 12 months

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11
Q

Process of testes descending in male dogs

A

Born with them intra-abdominally
Move through inguinal canal by 3-4 days after birth
Descend by 2 weeks after birth
Final position in scrotum at 35 days

BUT can take longer

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12
Q

Puberty in queens

A

VERY weight dependent
See at 6-9 months

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13
Q

How is puberty onset in cats altered by being long day breeders

A

By photoperiod; may reach right AGE for puberty in winter BUT won’t achieve it until SPRING

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14
Q

Specific breed exceptions to normal puberty times in cats

A

Oriental breeds: reach puberty very early
Long haired and manx breeds reach it later (11-21 months)

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15
Q

Which breed of dog has retained seasonality in breeding

A

Bassen G

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16
Q

What is the oestrus cycle in the bitch like

A

Non-seasonally mono-oestrus
> Inter-oestrus interval variable; around 6-7 months

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17
Q

Exceptions in inter-oestrus interval in dog breeds

A

German shepherds and rottweilers: inter-oestrus interval just 4-5 months

Tibetin mastiff: cycles once per year

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18
Q

Reproductive cycle in the bitch; brief summary

A

Anoestrus: get small peaks of FSH but not enough to stimulate growth
- THEN: start to get LH in circulation which allows follicles to mature

Enter proestrus: follicles are producing oestrogens; get blood discharge from vagina and vulva enlargement (NOT RECEPTIVE)
- Then discharge gets paler and vulva less oedematous

Estrus: standing heat; will put tail to side to expose genitalia

After ovulation: enter luteal phase (dioestrus) = sexual quiescence

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19
Q

Which breeds have lots of vulval swelling; and what is normal

A

Normal is 2-3 x dioestrus size

Boxers show lots of swelling

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20
Q

What is significant about progesterone production in the bitch

A

Granulosa cells lining follicle start to produce progesterone before ovulation; so can use this to predict the timing of ovulation

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21
Q

WHy does fertilisation in the bitch not happen until 48-72 hours after ovulation

A

Because the oocyte that ovulates is IMMATURE; still in meiosis 2 i.e a primary oocyte

Takes time to complete this to be competent for fertilisation

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22
Q

How long is the luteal phase is a non-pregnant bitch

A

Almost identical to in a pregnant bitch i.e 45-70 days (~2 months)

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23
Q

How long is the follicular phase in dogs

A

18 days;
Proestrus ~ 9 days
Oestrus ~ 9 days

24
Q

When does a bitch ovulate in relation to oestrus

A

On day 2 of oestrus; i.e 11 days after start of proestrus (blood)

25
What is a practical approach to when to breed a bitch in relation to start of proestrus (first blood)
Day 9, 11 and 13
26
What vaginal exfoliate cytology do we see in proestrus
RBCs Non-cornified epithelial cells; large with lots of cytoplasm and visible nucleus
27
What vaginal exfoliate cytology do we see in oestrus
Epithelial cells starting to die and be shed = cornified epithelial cells which look like fish scales > Bitch in 'oestrus' when 50% of cells are cornified
28
What is the indicator cell for metostrus on vaginal cytology
neutrophils = too late for breeding
29
How does vaginal mucosa look (via vaginoscopy) through different phases of the cycle
Early proestrus: pale pink, no oedema Late protestus: very pale; LOTS OF SWELLING Oestrus: very pale and wrinkled, no swelling = like funnel with completely flat mucosa and can see to cervix Dioestrus: vortex; pink, no swelling
30
Using hormonal tests to work out when ovulation is
Measure progesterone levels daily from 1 week after blood discharge starts Take ovulation to be when there is a sudden rise of 3-5ng/ml in P Or measure LH; from 5 days after bleeding; until you measure the surge
31
When should we breed bitch in relation to ovulation timing
2 days after ovulation
32
What kind of oestrus cycle does the domestic queen have
Seasonally poly-oestrus Starts cycling around jan/feb (may not be seasonal if indoors the whole time)
33
What is different about ovulation in domestric queen c/f bitch
INDUCED OVULATOR; ovulates after mating And usually needs >1 mating to stimulate ovulation
34
What is meant by term 'pseudopregnancy' in the domestic queen
When queens that ovulate don't conceive enter shorter luteal phase than if pregnancy Luteal phase is ~40 days in non-pregnant
35
Gestation period in a dog
57-72 days post breeding - Variation because we don't know at what point ovulation and fertilisation occurred Counting from LH surge; 65 days
36
Gestation period in a cat
57-72 days - Variation due to multiple matings being involved in inducing ovulation
37
How does multiple affect LH surge
MUCH greater c/f single mating Only 50% would ovulate from single mating
38
What are some influences on the duration of pregnancy in dogs
Number of fetuses; fewer means longer duration Body weight; heavier bitches pregnancy for longer
39
What is single pup syndrome
Getting an oversized single pup because gestation longer with just a single pup
40
What type of placenta do dogs and cats have
Endotheliochorial Zonary placenta
41
What proportion of antibody transfer happens in utero vs from colostrum
Just 5% in utero Rest from colostrum
42
Why can't we use progesterone levels to detect pregnancy in cats and dogs
BEcause very similar whether pregnant on not - In cats; we only get a significant difference later on in pregnancy at which point you can palpate the fetuses
43
When are we able to palpate a pregnancy in dogs vs cats
Dogs: 4 weeks post mating Cats: 3 weeks post mating
44
When can we detect pregnancy via X ray
After skeletal mineralisation In dogs: 45 days In cats: 40 days
45
When can we use ultrasound to detect a pregnancy
From ~18-20 days in dogs ~16 days in cats
46
What is a test we can use for pregnancy in dogs but not cats
Relaxin test from day 20
47
What is the max interval between pups being born
1 hour
48
What colour are placentas in dogs vs cats
Dogs: green/black Cats: brown
49
Stages of labour and timings in the bitch
Stage 1 = cervical dilatation + uterine contractions; 4-36 hours Stage 2 = expulsion of foetuses; pups normally born every 30-60 mins Stage 3 = expulsion of placentas; may be after each pup or at end
50
Stages of labour and timings in the queen
Stage 1 takes 24 hours Stage 2; HUGE VARIATION IN TIME > Kittens born every 5-60 mins Stage 3: brown placentas Labour can take 36 hours
51
When do we worry during parturition
If no labour by 68-70 days post-mating If strong abdominal straining but don't get first pup/kitten within 30-40 mins If more than 2 hours between first and second pup/kitten Green discharge and no pup within 30 mins of this; placental separation so stress Lots of bloody discharge 1
52
What drug can we give to neonates to stimulate and breathing and what must we be aware of
1-2 drops doxapram BUT be careful of overdose as risk of hyperventilation and cerebral hypoxia
53
What level of daily weight gain do we want in newborns
10% MINIMUM
54
Eyelid and ear canals opening
Takes 2 weeks in dogs 9 days in cats
55
How long does uterine involution take in bitches
4-5 weeks
56
Uterine involution in the queen
INfluenced by lactation Can be cycling again by 4 weeks post-partum if not lactating
57
Post birth discharge in bitches vs queens
Bitches: green/red --> brown Queens: not much