short quiz?

Question 1

____: orderly increase of all chemical constituents of cell

1. entails the replication of all ____, _____ & ______ from the nutrients present in the surrounding environment
2. when nuclear division is not accompanied by cell division
   - aka
3. when microorganism reproduce processes like budding or binary fission

Answer 1

Bacterial Growth

1. cellular structures, organelle, protoplasmic components
2. increase in cell size
   - coenocytic
3. increase in cell number

Question 2

Stages of Bacterial Growth

_____: little to no multiplication

1. ____ are very active
2. period of _____
3. start of _____
4. no cell division since they are still in _____

Answer 2

Lag Phase

1. enzymes
2. Rejuvenescence
3. biosynthesis
4. adjustment phase

Question 3

Stages of Bacterial Growth

_____: organisms grow at maximum rate

1. aka
2. most active in reproduction happens during this phase, hence, performing ____ is best during this phase
3. phase where they are most susceptible to _____

Answer 3

Logarithmic (Log) Phase

1. Exponential Phase
2. biochem tests
3. antibiotics

Question 4

Stages of Bacterial Growth

_____: growth ceases as nutrients are exhausted

1. aka
2. ____ products have accumulated
3. ____ = _____
4. period of ____

Answer 4

Stationary phase

1. Plateau phase
2. toxic metabolic products
3. cell division = cell death
4. period of equilibrium

Question 5

Stages of Bacterial Growth

_____: direct microscopic count may remain but viable count slowly decreases

1. aka
2. ___ > ____
3. there’s marked increased _____

Answer 5

Death Phase

1. Decline phase
2. dead cells > viable cells
3. marked increased death rate

Question 6

Stages of Bacterial Growth
1. Highest metabolic activity but without cell division:
2. Fastest growth:
3. Highest sensitivity to β-lactam:
4. Best to perform biochemical test:

Answer 6

1. Lab phase
2. Log phase
3. Log phase
4. Log phase

Question 7

1. doubling of the cell number through binary fission
2. time required for bacteria to double their population
3. generation time formula
4. final count formula
5. # of generations formula

Answer 7

1. Generation
2. Generation Time
3. t/n (# of generations)
4. Initial count x 2^# of generations
5. (LogN - LogNo) / Log2

Question 8

most common asexual reproductive process in which single cell divides into two daughter cells after developing transverse cell wall

Answer 8

Transverse Binary Fission

Question 9

Growth Measurement
1. through colony count then multiply it by 1000 = CFU or through direct microscopy
2. indirect estimation of bacterial growth; through turbidimetry
3. through biochemical activity of bacteria

Answer 9

1. By cell count
2. By cell mass
3. By cell activity