Sets 12 Flashcards
what are the three big differences between DNA & RNA
DNA:
double stranded
deoxyribose
having thymine
RNA:
single stranded
ribose
has uracil
is DNA or RNA less stable ?
RNA
what is the 3 differences between Protein & RNA+ DNA?
has an N (amino) terminus and C(Carboxy) terminus
multiple structure
1/20 AA instead of 1/4 Comp Base pairs
what are the 4 structure of proteins and what does it look like
Primary: single straight chain
Secondary: local folding single alpha helix or beta sheet
Tertiary: overall shape of the chain (both alpha & beta sheet folds)
Quaternary: multiple chains together
what the stop codes for DNA?
TAG
TGA
TAA
Whats the stop codes for RNA
UAG
UGA
UAA
what is the start code for DNA
ATG
what is the start code for RNA
AUG
what is the template strand
the strand being used for transcription
what is the coding strand ?
the RNA like strand , is not being used
what is the reading frame
from AUG to one of the stop codons
what is a frame shape mutations and what are the two example ?
single base deletion: removing a base
single base addition: adding a base
it where the frame shifts
how would you correct shift mutation and explain it ?
intragenic supression ; restoration of gene function by one mutation that cancel the other in the gene
how did they figure out which codons make which amino acid
- they added synthetic (fake) mRNA with a known sequence in vitro
- they use the radioactivity of the amino acids attached to the tRNA to depict the exact sequence
is the genetic code universal ?
almost universal there are some ciliates and mitochondria who follow a different rule