Sets 12 Flashcards

1
Q

what are the three big differences between DNA & RNA

A

DNA:
double stranded
deoxyribose
having thymine

RNA:
single stranded
ribose
has uracil

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2
Q

is DNA or RNA less stable ?

A

RNA

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3
Q

what is the 3 differences between Protein & RNA+ DNA?

A

has an N (amino) terminus and C(Carboxy) terminus
multiple structure
1/20 AA instead of 1/4 Comp Base pairs

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4
Q

what are the 4 structure of proteins and what does it look like

A

Primary: single straight chain
Secondary: local folding single alpha helix or beta sheet
Tertiary: overall shape of the chain (both alpha & beta sheet folds)
Quaternary: multiple chains together

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5
Q

what the stop codes for DNA?

A

TAG
TGA
TAA

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6
Q

Whats the stop codes for RNA

A

UAG
UGA
UAA

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7
Q

what is the start code for DNA

A

ATG

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8
Q

what is the start code for RNA

A

AUG

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9
Q

what is the template strand

A

the strand being used for transcription

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10
Q

what is the coding strand ?

A

the RNA like strand , is not being used

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11
Q

what is the reading frame

A

from AUG to one of the stop codons

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12
Q

what is a frame shape mutations and what are the two example ?

A

single base deletion: removing a base
single base addition: adding a base
it where the frame shifts

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13
Q

how would you correct shift mutation and explain it ?

A

intragenic supression ; restoration of gene function by one mutation that cancel the other in the gene

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14
Q

how did they figure out which codons make which amino acid

A
  1. they added synthetic (fake) mRNA with a known sequence in vitro
  2. they use the radioactivity of the amino acids attached to the tRNA to depict the exact sequence
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15
Q

is the genetic code universal ?

A

almost universal there are some ciliates and mitochondria who follow a different rule

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