Session 8- COVID-19

Question 1

What doe viruses consist of

Answer 1

Delivery system- protects it against degradation in the environment and contains structures used to bind to target cells

Payload- contains the genome and enzymes necsssary to initiate the first step in replication

Question 2

Structure of Coronavirus

Answer 2

Spike glycoproteins

Membrane protein

Envelope glycoprotein

Nuclepcapsid protein

Lipid bilayer

Question 3

Genetic material of corona virus

Answer 3

SsRNA-

Spike S glycoproteins required for entry into cells

Question 4

Droplet transmission

Answer 4

Coughs and sneezes ca spread droplets of saliva and mucus

Question 5

Airborne transmission

Answer 5

Tiny particles possible produced by talking are suspended in the air for longer and travel further

Question 6

How does the virus enter cells

Answer 6

ACE2 a transmembrane protein which is widely expressed in lung heart kidney and GI tract which is a receptor for the virus

Entry then occurs via receptor-mediated endocytosis

Question 7

Replication of coronavirus

Answer 7

+ SsRNA turns to -SsRNA via RNA-dependant RNA polymerase then back to + SsRNA

At the same time viral proteins are made which assembly into nucelocapsisds

Question 8

Symptoms and complications of COVID-19

Answer 8

SOB
Pneumonia 
Septic shock
Renal failure 
Diarrhoea 
Myalgia 
Cough 
Hemoptysis 
Fever/ headache

Question 9

Molecular COVID test

Answer 9

Obtain specimen

Extract RNA from specimen and convert to DNA

Amplify by PCR with SARS-Cov-2 specific primers

Interpret results

Question 10

Salivadirect test for Covid

Answer 10

Obtain specimen

RNA-extraction free process. Convert RNA from specimen to DNA and amplify by PCR with SARS-Cov-2 primers