Session 1 Flashcards
Define tissue
A collection of cells specialised to perform a particular function.
Aggregations of tissues constitute organs.
Define biopsy
The removal of a small piece of tissue from an organ or part of the body for microscopic examination.
What is a smear biopsy?
Collect cells by spontaneous/mechanical exfoliation. Smear on slide.
When are smear biopsys used?
Cervix, buccal cavity.
What is a Curettage biopsy?
Remove tissue by scooping/scraping.
When is a Curettage biopsy used?
Endometrial lining of uterus
What is a needle biopsy?
Put needle into tissue to gather cells.
When is a needle biopsy used?
Brain, breast, liver, kidney, muscle.
What is a direct incision biopsy?
Cut directly into tissue of interest and remove tissue.
When is a direct incision biopsy used?
Skin, mouth, larynx.
What is an endoscopic biopsy?
Removal of tissue via instruments through an endoscope.
When is an endoscopic biopsy used?
Lung, intestine, bladder.
When is transvascular biopsy used?
Heart, Liver
Define fixed biopsy
Macromolecules cross-linked, cellular structure preserved, no autolysis or putrefaction.
Commonly used fixatives include glutaraldehyde and formaldehyde.
Define Shrinkage artifacts
Abnormalities on the final slide due to preparation process involving dehydrated and then rehydrated.
How does Haematoxylin and Eosin (H&E) work?
Haematoxylin stains acidic components of cells, eg Nucleolus (RNA), Chromatin (DNA) purple/blue.
Eosin stains basic components of cells, eg most cytoplasmic proteins and extracellular fibres pink
How does Periodic Acid-Schiff (PAS) work?
Stains carbohydrates and glycoproteins magenta.
What is phase contrast microscopy?
Uses interference effects of two combining light waves