Serology Flashcards
Non treponemal/Nonspecific methods for Syphilis serologic tests
VDRL
RPR
Wasserman test
VDRL
Venereal Disease Research Laboratory
RPR
Rapid Plasma Reagin
nonserologic test for syphilis that uses Charcoal
RPR
nonserologic test for syphilis that is read Macroscopically
RPR
Reagent for RPR
Charcoal - visualization
choline chloride - added for heating, inactivator
Thimerosal - preservative
RPR rotator
100 RPM per 8 mins
RPR ring diameter
18 mm
Antigen delivery needle gauge
20 gauge ( 60 drops/mL)
Principle for RPR
Flocculation
Principle for VDRL
Flocculation
Wasserman Test developed by
August Paul von Wasserman
Wasserman Test ANTIGEN
Crude Liver Extract
Crude Liver Extract is from
fetus infected with syphilis
principle of Wasserman test
Complement fixation test
(+) NO HEMOLYSIS
BEST test to monitor therapeutic response is
nontreponemal test because reagin decreases
VDRL specimen
heated (inactivated) serum
VDRL is read thru
microscopic exam
Specimen for neurosyphilis
CSF
Reagent for VDRL
Cardiolipin, Lecithin, Cholesterol
rotator in VDRL
180 RPM for 4 mins (serum)
180 RPM for 8 minutes (CSF)
Ring diameter in VDRL
14 mm (serum)
16 mm (CSF)
depth: 1.75 mm
reagent in VDRL that enhances reacting surface
Cholesterol
gauge for VDRL quantitative
gauge 19
gauge for VDRL qualitative
gauge 18
gauge for VDRL CSF
gauge 21 or 22
VDRL:
No clumps
nonreactive
VDRL:
medium to large clumps
Reactive
VDRL:
small clumps
Weakly reactive
false positive in VDRL results
SLE, RA, IM, Malaria, Pregnancy
