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Blood Bank - review > Serology > Flashcards

Serology Flashcards

1
Q

Non treponemal/Nonspecific methods for Syphilis serologic tests

A

VDRL
RPR
Wasserman test

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2
Q

VDRL

A

Venereal Disease Research Laboratory

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3
Q

RPR

A

Rapid Plasma Reagin

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4
Q

nonserologic test for syphilis that uses Charcoal

A

RPR

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5
Q

nonserologic test for syphilis that is read Macroscopically

A

RPR

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6
Q

Reagent for RPR

A

Charcoal - visualization
choline chloride - added for heating, inactivator
Thimerosal - preservative

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7
Q

RPR rotator

A

100 RPM per 8 mins

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8
Q

RPR ring diameter

A

18 mm

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9
Q

Antigen delivery needle gauge

A

20 gauge ( 60 drops/mL)

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10
Q

Principle for RPR

A

Flocculation

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11
Q

Principle for VDRL

A

Flocculation

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12
Q

Wasserman Test developed by

A

August Paul von Wasserman

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13
Q

Wasserman Test ANTIGEN

A

Crude Liver Extract

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14
Q

Crude Liver Extract is from

A

fetus infected with syphilis

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15
Q

principle of Wasserman test

A

Complement fixation test

(+) NO HEMOLYSIS

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16
Q

BEST test to monitor therapeutic response is

A

nontreponemal test because reagin decreases

17
Q

VDRL specimen

A

heated (inactivated) serum

18
Q

VDRL is read thru

A

microscopic exam

19
Q

Specimen for neurosyphilis

A

CSF

20
Q

Reagent for VDRL

A

Cardiolipin, Lecithin, Cholesterol

21
Q

rotator in VDRL

A

180 RPM for 4 mins (serum)

180 RPM for 8 minutes (CSF)

22
Q

Ring diameter in VDRL

A

14 mm (serum)

16 mm (CSF)

depth: 1.75 mm

23
Q

reagent in VDRL that enhances reacting surface

A

Cholesterol

24
Q

gauge for VDRL quantitative

A

gauge 19

25
Q

gauge for VDRL qualitative

A

gauge 18

26
Q

gauge for VDRL CSF

A

gauge 21 or 22

27
Q

VDRL:

No clumps

A

nonreactive

28
Q

VDRL:

medium to large clumps

A

Reactive

29
Q

VDRL:

small clumps

A

Weakly reactive

30
Q

false positive in VDRL results

A

SLE, RA, IM, Malaria, Pregnancy

Blood Bank - review (7 decks)

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