Antihuman Globulin Test Flashcards

1
Q

other term for AHG test

A

Coomb’s test

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2
Q

how is it discovered

A

inject human serum into rabbits

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3
Q

developed by

A

Coombs and coworkers

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4
Q

color of AHG reagent

A

Green

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5
Q

types of AHG reagents

A

Polyspecific

Monospecific

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6
Q

type of AHG reagent that contains both anti-IgG and anti-C3d

A

Polyspecific

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7
Q

type of AHG reagent that contains either anti-IgG oranti-C3d

A

Monospecific

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8
Q

two types of AHG tests

A

Direct Antiglobulin Test (DAT)

Indirect antiglobulin test (IAT)

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9
Q

Detects in vivo sensitization of RBCs

A

DAT

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10
Q

detects in vitro sensitization of RBCs

A

IAT

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11
Q

Positive result for DAT

A

Visible agglutination

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12
Q

Positive result for IAT

A

Visible agglutination

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13
Q

difference of IAT from DAT

A

IAT requires incubation

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14
Q

percent of RCS used

A

2 - 5%

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15
Q

DAT Specimen

A

EDTA whole blood

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16
Q

DAT is for detection of

A

HDN
HTN
AIHA

17
Q

IAT is for detection of

A

Antibody screening and identification
Antibody titration
RBC phenotyping
Weak D testing

18
Q

Sources of error in AHG Tests (False-Positive)

A

improper specimen (ref or clotted)
overcentrifugation
bacterial contam
dirty glassware

19
Q

Sources of error in AHG Tests (False-Negative)

A

Inadequate or improper washong of cells
undercentrifugation
serum:cell ratio not ideal
AHG reagent not added

20
Q

what to do to prevent NEUTRALIZATION

A

RBCs must be saline-washed at least 3 times before adding AHG

21
Q

Coombs control cells are

A

O positive RBCs coated with anti-D

22
Q

TRUE/FALSE

Antiglobulin test reports NEGATIVE instantly

A

FALSE

*requires checking by use of Coombs control cells