Antihuman Globulin Test Flashcards
other term for AHG test
Coomb’s test
how is it discovered
inject human serum into rabbits
developed by
Coombs and coworkers
color of AHG reagent
Green
types of AHG reagents
Polyspecific
Monospecific
type of AHG reagent that contains both anti-IgG and anti-C3d
Polyspecific
type of AHG reagent that contains either anti-IgG oranti-C3d
Monospecific
two types of AHG tests
Direct Antiglobulin Test (DAT)
Indirect antiglobulin test (IAT)
Detects in vivo sensitization of RBCs
DAT
detects in vitro sensitization of RBCs
IAT
Positive result for DAT
Visible agglutination
Positive result for IAT
Visible agglutination
difference of IAT from DAT
IAT requires incubation
percent of RCS used
2 - 5%
DAT Specimen
EDTA whole blood