serological test for syphilis

Question 1

also known as the italian disease or french disease

Answer 1

syphilis

Question 2

syphilis is a sexually transmitted disease caused by the spirochete __

Answer 2

treponema pallidum subsp. pallidum

Question 3

syphilis marked by the developmen of a hard, painless, and solitary genital lesion called chancre or hunterian chancre

Answer 3

primary syphilis

Question 4

primary syphilis appears between __ days after the initial infection

Answer 4

10-90 days

Question 5

secondary syphilis begins __ weeks after the disappearance of the chancre

Answer 5

2-12 weeks

Question 6

syphilis wherein if left untreated, lymphadenopathy, fever, pharyngitis, malaise, and rashes on the skin and mucous membrane may develop

Answer 6

secondary syphilis

Question 7

syphilis wherein wart-like lesions called condyloma lata may be seen on the genital areas

Answer 7

secondary syphilis

Question 8

syphilis characterized by the disappearance of the signs and symptoms of syphilis; patients generally non-infectious

Answer 8

latent syphilis

Question 9

tertiary syphilis occurs approximately __ years after secondary syphilis

Answer 9

10-30 years

Question 10

syphilis where patients experience cardiovascular disease, neurosyphilis, and localized subcutaneous granulomas called gummas

Answer 10

tertiary syphilis

Question 11

dianosis of syphilis is done through the demonstration of the microorganism using __ microscopy and serology

Answer 11

dark-field

Question 12

screening tests with a high sensitivity for the diagnosis of syphilis

Answer 12

non-treponemal test

Question 13

involves detection of reagin using known suspensions of cardiolipin, which is prepared from the alcoholic extract of beef cardiac muscle

Answer 13

non-treponemal test

Question 14

highly specific and sensitive tests that detect treponemal antibodies

Answer 14

treponemal test

Question 15

serve as verification procedures for validating results obtained in a non-treponemal test

Answer 15

treponemal test

Answer 16

non-treponemal

Answer 17

non-treponemal

Answer 18

non-treponemal

Answer 19

treponemal

Answer 20

treponemal

Answer 21

treponemal

Question 22

serum vdrl slides (__ inches) with 12 paraffin or ceramic-coated rings approximately __mm in diameter

Answer 22

2x3 inches, 14mm

Question 23

VDRL syringe used

Answer 23

leuer type with a gauge hypodermic needle (18g, no bevel)

Question 24

t/f: serum is inactivated in VDRL before the procedure

Answer 24

t

Question 25

gauge of needle used in VDRL (qualitative test)

Answer 25

18g

Question 26

gauge of needle used in VDRL (quantitative test)

Answer 26

19g

Question 27

gauge of needle used in VDRL (CSF)

Answer 27

21 or 22g

Question 28

gauge of needle used in RPR card

Answer 28

20g

Question 29

concavity slide for VDRL (CSF)

Answer 29

boerner

Question 30

in VDRL, slide is rotated for __ mins on a mechanical rotator set at __ rpm

Answer 30

4 mins, 180rpm

Question 31

in VDRL test results are read under __

Answer 31

LPO

Question 32

t/f: VDRL test should be performed at RT

Answer 32

t

Question 33

[manner of reporting - VDRL]: no clumping

Answer 33

non-reactive; -

Question 34

[manner of reporting - VDRL]: no clearly defined clumps

Answer 34

borderline; +/-

Question 35

[manner of reporting - VDRL]: small but clearly detectable clumps

Answer 35

weakly reactive; 1+

Question 36

[manner of reporting - VDRL]: medium and large clumps

Answer 36

reactive; 2+, 3+, 4+

Question 37

disposable plastic-coated test cared of RPR has 10 test circles of __ diameter

Answer 37

18mm

Question 38

dispensing device used in RPR

Answer 38

dispenstir

Question 39

[manner of reporting - RPR]: smooth, even grey background with no floccules visible

Answer 39

non-reactive

Question 40

[manner of reporting - RPR]: presence of small black floccules around the edge of the test circle, white background

Answer 40

weakly reactive

Question 41

[manner of reporting - RPR]: large and medium black floccules against a white background

Answer 41

reactive

Question 42

t/f: control sera are always included in the RPR testing period to ensure proper reactivitiy of the antigen emulsion at the time the tests are conducted

Answer 42

t

Question 43

t/f: in non-treponemal tests, a negative result indicates that an individual is uninfected

Answer 43

f

Question 44

t/f: there is no correlation between the amount of reagin in a patient's serum and the severity of the disease

Answer 44

t

Question 45

reagin antibody is not usually detected by the VDRL and RPR test until about __ weeks after infection `4-5 weeks

Answer 45

4-5 weeks

Question 46

reagin levels peak during the __ of the disease and decline to a stable level during late syphilis

Answer 46

secondary stage

Question 47

when paired sera are tested, a __ or greater rise in reagin titer is suggestive of __

Answer 47

four-fold, syphilis

Question 48

a reagin titer of __ suggests latent infection, previous treatment, or late syphilis

Answer 48

<1:8

Question 49

t/f: serum must be inactivated before undergoing TPPHA test

Answer 49

f

Question 50

[manner of reporting]: definite button of non-agglutinated cells with or without a very small hole in the center of the test well

Answer 50

negative/non-reactive

Question 51

[manner of reporting]: button of cells with a small hole in the center, giving the appearance of a well-defined, thick, and dense ring with a clear background

Answer 51

indeterminate (retest)

Question 52

[manner of reporting]: a smooth may of agglutinated cells or a smooth mat surrounded by a circle of aggregated cells

Answer 52

reactive